{"title":"DNAzyme@MOF breaking pH limitation for the detection of dopamine in the interstitial fluid","authors":"Jiuhong Zhao, Jinnan Zhao, Xiaoyu Zhang, Guixia Ling, Peng Zhang","doi":"10.1016/j.bios.2025.117367","DOIUrl":null,"url":null,"abstract":"<div><div>The level of dopamine (DA) in the human body has a certain correlation with neurological diseases. However, most detection methods of DA are complex and expensive. In this study, laccase-like DNAzyme@MOF with improved pH stability was successfully prepared. DNAzyme@MOF could catalyze the chromogenic substrate to change the color of the solution for the detection of DA in ISF. The addition of DNAzyme made DNAzyme@MOF possess higher stability and enzyme-like activity. The operation process was simple, rapid, and intuitive. In addition, the <em>in vivo</em> DA content in skin interstitial fluid (ISF) was analyzed by an off-line method. The swelling hydrogel microneedles (MNs) were prepared to extract skin ISF. DA in skin ISF was recovered and detected by laccase-like DNAzyme@MOF. This study realized the minimally invasive detection of DA. The proposed detection method of biomarkers in ISF based on DNAzyme@MOF would provide a new dimension towards the future development for the detection of other biomarkers in ISF.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"279 ","pages":"Article 117367"},"PeriodicalIF":10.5000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biosensors and Bioelectronics","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0956566325002416","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/3/17 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"BIOPHYSICS","Score":null,"Total":0}
引用次数: 0
Abstract
The level of dopamine (DA) in the human body has a certain correlation with neurological diseases. However, most detection methods of DA are complex and expensive. In this study, laccase-like DNAzyme@MOF with improved pH stability was successfully prepared. DNAzyme@MOF could catalyze the chromogenic substrate to change the color of the solution for the detection of DA in ISF. The addition of DNAzyme made DNAzyme@MOF possess higher stability and enzyme-like activity. The operation process was simple, rapid, and intuitive. In addition, the in vivo DA content in skin interstitial fluid (ISF) was analyzed by an off-line method. The swelling hydrogel microneedles (MNs) were prepared to extract skin ISF. DA in skin ISF was recovered and detected by laccase-like DNAzyme@MOF. This study realized the minimally invasive detection of DA. The proposed detection method of biomarkers in ISF based on DNAzyme@MOF would provide a new dimension towards the future development for the detection of other biomarkers in ISF.
期刊介绍：
Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.
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