A Rapid and Sensitive SERS-Based Au/MIL-125(Ti) Biosensor for Hyperuricemia Diagnosis via Saliva Analysis

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL Analytical Chemistry Pub Date : 2025-03-19 DOI:10.1021/acs.analchem.4c05499

Yifan Liu, Miao Li, Zhenxia Zhao, Zhongxing Zhao, Min Li

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Abstract

An Au/MIL-125(Ti) nanocomposite was developed as a novel Raman-based biosensor platform for the noninvasive evaluation of uric acid (UA) levels in saliva. The intensity ratio (I₄₉₇/I₁₆₄₈) for the Raman spectrum of uric acid was found to exhibit a quantitative correlation with uric acid concentration upon its adsorption onto MIL-125(Ti). This innovative method achieves an impressive limit of detection (LOD) of 0.01 μM with consistent performance in physiological media. This platform shows a wide linear detection range from 50 μM to 5000 μM, showcasing its sensitivity and versatility. Furthermore, this biosensor facilitates rapid UA detection in saliva samples and can distinguish clearly hyperuricemia sufferers from healthy controls within a remarkable time frame of only 90 s without complicated pretreatments, highlighting its great potential for real-time diagnostics. This research underscores the transformative potential of composite biosensors based on metal–organic frameworks (MOFs) for biomarker sensing applications, paving the way for future innovations in noninvasive diagnostic technologies.

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基于sers的Au/MIL-125（Ti）生物传感器用于唾液分析诊断高尿酸血症

研究了一种Au/MIL-125（Ti）纳米复合材料，作为一种新型的基于拉曼的生物传感器平台，用于无创评估唾液中尿酸（UA）水平。尿酸在MIL-125（Ti）上吸附后，其拉曼光谱强度比（I497/I1648）与尿酸浓度呈定量相关。这种创新方法的检测限（LOD）达到了令人印象深刻的0.01 μM，在生理介质中表现一致。该平台具有50 μM到5000 μM的宽线性检测范围，显示了其灵敏度和通用性。此外，这种生物传感器有助于在唾液样本中快速检测UA，并且可以在仅90秒的时间框架内明确区分高尿酸血症患者和健康对照组，而无需复杂的预处理，突出了其实时诊断的巨大潜力。这项研究强调了基于金属有机框架（mof）的复合生物传感器在生物标志物传感应用中的变革潜力，为未来无创诊断技术的创新铺平了道路。

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.