Coumarin-based fluorescent probe for hypochlorite detection and imaging of acute kidney injury

Abstract

Acute kidney injury (AKI) has attracted attention as a frequent clinical disease, and the occurrence of AKI can lead to the decline of kidney function and even endanger life. Realizing early diagnosis of AKI can effectively control and treat the condition. Current clinical diagnostic methods cannot meet the accurate diagnosis of early acute kidney injury. Oxidative stress has been proved to be a causative factor of AKI. Therefore, we constructed a fluorescent probe based on coumarin fluorophore for the detection of hypochlorite (HClO) produced during oxidative stress to realize early diagnosis of AKI. The structure of the probe is simple and easy to synthesize, and it has the ability of colorimetric and fluorescence recognition for HClO. The probe responds to HClO as fast as 3 seconds and shows good selectivity and sensitivity to HClO with a detection limit of 15.6 nM. The probe is also well used for imaging exogenous and endogenous HClO in cells. It also can be successfully used for imaging and diagnosis of cisplatin-induced acute kidney injury.