Optical sensing devices are utilized for noncontact operation in harsh environments due to their advantage of local separation between the measurement and detector systems. Cholesteric liquid crystals (CLCs) with vivid structural colors are integrated into optical sensors, attracting significant interest for naked-eye detection. To enable vision-based, real-time remote monitoring, we propose a cost-effective method to convert the stimuli-responsive reflection change of CLCs into recordable diffraction patterns using polarization volume gratings (PVGs). This enables real-time remote readout of environmental parameters. PVGs are constructed through holographic polarization interference and photoalignment technologies. The diffraction efficiency of PVGs varies with relative humidity (RH). To improve sensing accuracy and extend the monitoring range of RH, we employ two methods: an orthogonal grating structure and dual-wavelength detection optical path. In addition, we demonstrate the simultaneous measurement of humidity and temperature by cascading independently responding PVGs. We believe that this work will provide a broader perspective and expand the application of light dimensions in LC-based optical sensing.
{"title":"Simultaneous monitoring of humidity and temperature by polarization volume gratings utilizing responsive cholesteric liquid crystals","authors":"Zhao-Yi Chen, Hao Yang, Shi-Long Li, Sen-Sen Li, Lu-Jian Chen","doi":"10.1016/j.snb.2024.136879","DOIUrl":"https://doi.org/10.1016/j.snb.2024.136879","url":null,"abstract":"Optical sensing devices are utilized for noncontact operation in harsh environments due to their advantage of local separation between the measurement and detector systems. Cholesteric liquid crystals (CLCs) with vivid structural colors are integrated into optical sensors, attracting significant interest for naked-eye detection. To enable vision-based, real-time remote monitoring, we propose a cost-effective method to convert the stimuli-responsive reflection change of CLCs into recordable diffraction patterns using polarization volume gratings (PVGs). This enables real-time remote readout of environmental parameters. PVGs are constructed through holographic polarization interference and photoalignment technologies. The diffraction efficiency of PVGs varies with relative humidity (RH). To improve sensing accuracy and extend the monitoring range of RH, we employ two methods: an orthogonal grating structure and dual-wavelength detection optical path. In addition, we demonstrate the simultaneous measurement of humidity and temperature by cascading independently responding PVGs. We believe that this work will provide a broader perspective and expand the application of light dimensions in LC-based optical sensing.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142563286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A sensitive and efficient photoelectrochemical (PEC) sensor formed with CdIn2S4 as photoanode and Cu2O/Au as photocathode was constructed for the quantitative monitoring of trenbolone (TB). CuSe with excellent performance was chosen as TB antigen (Ag) label. The correlation between photocurrent and TB concentration will be reflected through the competitive combining of TB antibodies (Ab) with TB or Ag-CuSe. The lower the TB concentration, the more Ag-CuSe was connected to the Ab, which eventually resulted in an augmentation in photocurrent. Under optimal conditions, the sensitive detection of TB was realized within the range of 100 fg/mL to 100 ng/mL with the limit of detection (LOD) of 10.3 fg/mL. The constructed PEC sensor possesses excellent selectivity and stability. This method provides a feasible and valuable way for detection of TB, showing great potential in environmental analysis.
{"title":"A competitive cathodic photoelectrochemical sensor based on a signal amplification of CdIn2S4 photoanode for trenbolone detection","authors":"Jinkun Yuan, Xin Chen, Hongmin Ma, Zhongfeng Gao, Xiang Ren, Rui Xu, Dan Wu, Qin Wei","doi":"10.1016/j.snb.2024.136883","DOIUrl":"https://doi.org/10.1016/j.snb.2024.136883","url":null,"abstract":"A sensitive and efficient photoelectrochemical (PEC) sensor formed with CdIn<sub>2</sub>S<sub>4</sub> as photoanode and Cu<sub>2</sub>O/Au as photocathode was constructed for the quantitative monitoring of trenbolone (TB). CuSe with excellent performance was chosen as TB antigen (Ag) label. The correlation between photocurrent and TB concentration will be reflected through the competitive combining of TB antibodies (Ab) with TB or Ag-CuSe. The lower the TB concentration, the more Ag-CuSe was connected to the Ab, which eventually resulted in an augmentation in photocurrent. Under optimal conditions, the sensitive detection of TB was realized within the range of 100 fg/mL to 100<!-- --> <!-- -->ng/mL with the limit of detection (LOD) of 10.3 fg/mL. The constructed PEC sensor possesses excellent selectivity and stability. This method provides a feasible and valuable way for detection of TB, showing great potential in environmental analysis.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142562306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Circulating tumor DNA (ctDNA) levels in the peripheral blood are correlated with tumor burden and malignant progression. Sensitive and accurate detection of ctDNA in human blood remains to be explored. This study introduces a novel approach that employs toehold-assisted ctDNA capture and hairpin assembly-mediated tripedal DNA walker, facilitating the sensitive detection of a broad range of ctDNAs. Specifically, the design includes a capture probe featuring an overhanging toehold domain for the identification of ctDNA, alongside three hairpin structures that incorporate blocked DNAzymes for the DNA walking mechanism. The capture probe identifies ctDNA through a toehold-mediated strand displacement reaction, which subsequently initiates the assembly of the three hairpin structures, resulting in the formation of a tripedal DNA walker. This tripedal walker is capable of initiating a walking process that generates detectable fluorescent signals for the quantification of ctDNA. The toehold-assisted ctDNA capture method demonstrates excellent accuracy and specificity for identical strands with a single-base mismatch, while the tripedal walker exhibits enhanced efficiency compared to bipedal and unipedal walkers. Furthermore, two stages of signal amplification were achieved, allowing for the sensitive detection of ctDNA with a detection limit of 0.3 fM in buffer solutions and 0.8 fM in serum samples. The quantitative analysis of ctDNA in human blood was effectively performed, demonstrating the method’s ability to distinguish between blood samples from healthy individuals and those from patients with tumor. Therefore, this method represents a significant advancement in ctDNA detection for clinical noninvasive liquid biopsies and holds considerable promise for tumor diagnosis.
{"title":"Toehold and Hairpin Assembly-Mediated Tripedal DNA Walker for Circulating Tumor DNA Detection in Human Blood","authors":"Wei Li, Jiayue Li, Shuaijing Wang, Manman Duan, Shang Sun, Wanling Cui, Zhenguang Wang","doi":"10.1016/j.snb.2024.136887","DOIUrl":"https://doi.org/10.1016/j.snb.2024.136887","url":null,"abstract":"Circulating tumor DNA (ctDNA) levels in the peripheral blood are correlated with tumor burden and malignant progression. Sensitive and accurate detection of ctDNA in human blood remains to be explored. This study introduces a novel approach that employs toehold-assisted ctDNA capture and hairpin assembly-mediated tripedal DNA walker, facilitating the sensitive detection of a broad range of ctDNAs. Specifically, the design includes a capture probe featuring an overhanging toehold domain for the identification of ctDNA, alongside three hairpin structures that incorporate blocked DNAzymes for the DNA walking mechanism. The capture probe identifies ctDNA through a toehold-mediated strand displacement reaction, which subsequently initiates the assembly of the three hairpin structures, resulting in the formation of a tripedal DNA walker. This tripedal walker is capable of initiating a walking process that generates detectable fluorescent signals for the quantification of ctDNA. The toehold-assisted ctDNA capture method demonstrates excellent accuracy and specificity for identical strands with a single-base mismatch, while the tripedal walker exhibits enhanced efficiency compared to bipedal and unipedal walkers. Furthermore, two stages of signal amplification were achieved, allowing for the sensitive detection of ctDNA with a detection limit of 0.3 fM in buffer solutions and 0.8 fM in serum samples. The quantitative analysis of ctDNA in human blood was effectively performed, demonstrating the method’s ability to distinguish between blood samples from healthy individuals and those from patients with tumor. Therefore, this method represents a significant advancement in ctDNA detection for clinical noninvasive liquid biopsies and holds considerable promise for tumor diagnosis.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142562308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fluorescence-based imaging has emerged as a promising technique for high-resolution visualization of latent fingerprints (LFPs), but detecting LFPs by fluorescent probes remains challenging at present. Herein, a water-soluble fluorescent dye (DEPN) with red emission and twisted intramolecular charge transfer (TICT) properties was synthesized, which function as a dual-mode in-situ imaging probe for LFPs. The desired solid composite fluorescent fingerprint powder DEPN@MMT can be obtained by milling with montmorillonite (MMT) to realize the imaging of LFPs with 1-3 levels of details, which has the advantages of simple preparation, low doping ratio, good photostability, and is not limited by the substrate. Comparison with the actual fingerprint details extracted by fingerprint reader revealed a good match between the both, indicating that the fingerprint powder has high application value. The permanent preservation of LFPs with different curvatures was also realized by making rubbings. In addition, DEPN was able to image LFPs in aqueous solution with a concentration of 50 μM over a wide pH range, and it took only 20 s to obtain high-quality images that met the NFIQ2 detection standard. This dual-mode imaging probe of LFPs is highly practical, offering adaptable detection patterns for a range of complicated situations. It provides a valuable method for rapid and convenient visualization of LFPs, as well as their subsequent preservation and analysis.
{"title":"Dual-mode visual imaging of latent fingerprints based on organic fluorescent probe with enhanced TICT emission","authors":"Zheng-Hong Pu, Jiao He, Xiang Liu, Jian Wang, Qing-Hong Bai, Cheng-Hui Wang, Xin Xiao","doi":"10.1016/j.snb.2024.136874","DOIUrl":"https://doi.org/10.1016/j.snb.2024.136874","url":null,"abstract":"Fluorescence-based imaging has emerged as a promising technique for high-resolution visualization of latent fingerprints (LFPs), but detecting LFPs by fluorescent probes remains challenging at present. Herein, a water-soluble fluorescent dye (DEPN) with red emission and twisted intramolecular charge transfer (TICT) properties was synthesized, which function as a dual-mode in-situ imaging probe for LFPs. The desired solid composite fluorescent fingerprint powder DEPN@MMT can be obtained by milling with montmorillonite (MMT) to realize the imaging of LFPs with 1-3 levels of details, which has the advantages of simple preparation, low doping ratio, good photostability, and is not limited by the substrate. Comparison with the actual fingerprint details extracted by fingerprint reader revealed a good match between the both, indicating that the fingerprint powder has high application value. The permanent preservation of LFPs with different curvatures was also realized by making rubbings. In addition, DEPN was able to image LFPs in aqueous solution with a concentration of 50<!-- --> <!-- -->μM over a wide pH range, and it took only 20<!-- --> <!-- -->s to obtain high-quality images that met the NFIQ2 detection standard. This dual-mode imaging probe of LFPs is highly practical, offering adaptable detection patterns for a range of complicated situations. It provides a valuable method for rapid and convenient visualization of LFPs, as well as their subsequent preservation and analysis.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-31DOI: 10.1016/j.snb.2024.136862
Carlos Benitez-Martin, Francisco Najera, Ezequiel Perez-Inestrosa
Fluorescence is a valuable tool for understanding mitochondria-lysosome interactions, which is central for unveiling their roles in health and disease. Herein, we describe novel indolium-based molecules for labelling mitochondria and lysosomes simultaneously with organelle-specific fluorescence properties. These probes readily enable clear distinction of mitochondria and lysosomes by combining confocal and two-photon fluorescence microscopy. Our results show that this unique behaviour relies on aggregation. The functioning of these probes is not compromised by mitophagy, allowing the visualisation of these organelles in separate emission channels by using a single fluorescent marker under all scenarios. Their enhanced experimental simplicity compared to using multiple dyes makes these novel compounds well-suited for automated screening applications within living cells.
{"title":"Simultaneous dual-colour labelling of mitochondria and lysosomes: an indolium-based approach","authors":"Carlos Benitez-Martin, Francisco Najera, Ezequiel Perez-Inestrosa","doi":"10.1016/j.snb.2024.136862","DOIUrl":"https://doi.org/10.1016/j.snb.2024.136862","url":null,"abstract":"Fluorescence is a valuable tool for understanding mitochondria-lysosome interactions, which is central for unveiling their roles in health and disease. Herein, we describe novel indolium-based molecules for labelling mitochondria and lysosomes simultaneously with organelle-specific fluorescence properties. These probes readily enable clear distinction of mitochondria and lysosomes by combining confocal and two-photon fluorescence microscopy. Our results show that this unique behaviour relies on aggregation. The functioning of these probes is not compromised by mitophagy, allowing the visualisation of these organelles in separate emission channels by using a single fluorescent marker under all scenarios. Their enhanced experimental simplicity compared to using multiple dyes makes these novel compounds well-suited for automated screening applications within living cells.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-31DOI: 10.1016/j.snb.2024.136871
Xiaoying Gao, Jiarui Zhu, Jiahui Zhao, Lei Zhao, Ying Sun, Jiazhen Lin, Mengli Hu, Yunjia Liu, Shenghong Yang, Jian Liu
A cascade colorimetric detection of salivary D-amino acids (DAAs) holds promise for the preliminary screening diagnosis of gastric cancer. Pursuing metal-organic complexes with high peroxidase-mimic (POD-mimic) activity is crucial to enhance diagnosis efficiency. In this work, we developed a straightforward strategy in a “one-pot” process to modulate the POD-mimic activity of CuX-trithiocyanuric acid (CuX-TTCA) complexes. By adjusting the molar ratios of CuX (Cl, Br, and I) and TTCA during synthesis to modify the coordination configuration of Cu(I) in CuX-TTCA, we easily tuned their POD-mimic activities. Among them, CuCl-TTCA-2 with a feeding molar ratio of 2:1 for CuCl:TTCA exhibited remarkable POD-mimic activity attributed to its exposed catalytic active sites and efficient mass transfer ability during catalysis. Long-lived 1O2 was identified as the primary reactive oxygen intermediate. A highly sensitive and selective cascade colorimetric detection platform was developed for two typical DAAs associated with gastric cancer, namely D-proline and D-alanine, achieving limit of detection values of 1.1 and 0.8 μM, respectively. As a proof-of-concept application, our cascade detection platform demonstrated excellent specificity in distinguishing saliva samples between gastric cancer patients and healthy individuals. The outstanding selectivity and reliable outcomes from DAAs assay make our detection platform highly promising for preliminary screening diagnosis of gastric cancer and patient self-detection applications. Our straightforward strategy for tuning POD-mimic activity provides an in-depth understanding on structure-activity relationship in nanozymes, offering valuable opportunities to advance enzyme-mimic optimization for other potential applications.
{"title":"Tuning the Peroxidase-Mimic Activity of CuX-Trithiocyanuric Acid Complexes for Colorimetric Detection of Gastric Cancer-Associated D-Amino Acids","authors":"Xiaoying Gao, Jiarui Zhu, Jiahui Zhao, Lei Zhao, Ying Sun, Jiazhen Lin, Mengli Hu, Yunjia Liu, Shenghong Yang, Jian Liu","doi":"10.1016/j.snb.2024.136871","DOIUrl":"https://doi.org/10.1016/j.snb.2024.136871","url":null,"abstract":"A cascade colorimetric detection of salivary D-amino acids (DAAs) holds promise for the preliminary screening diagnosis of gastric cancer. Pursuing metal-organic complexes with high peroxidase-mimic (POD-mimic) activity is crucial to enhance diagnosis efficiency. In this work, we developed a straightforward strategy in a “one-pot” process to modulate the POD-mimic activity of CuX-trithiocyanuric acid (CuX-TTCA) complexes. By adjusting the molar ratios of CuX (Cl, Br, and I) and TTCA during synthesis to modify the coordination configuration of Cu(I) in CuX-TTCA, we easily tuned their POD-mimic activities. Among them, CuCl-TTCA-2 with a feeding molar ratio of 2:1 for CuCl:TTCA exhibited remarkable POD-mimic activity attributed to its exposed catalytic active sites and efficient mass transfer ability during catalysis. Long-lived <sup>1</sup>O<sub>2</sub> was identified as the primary reactive oxygen intermediate. A highly sensitive and selective cascade colorimetric detection platform was developed for two typical DAAs associated with gastric cancer, namely D-proline and D-alanine, achieving limit of detection values of 1.1 and 0.8<!-- --> <!-- -->μM, respectively. As a proof-of-concept application, our cascade detection platform demonstrated excellent specificity in distinguishing saliva samples between gastric cancer patients and healthy individuals. The outstanding selectivity and reliable outcomes from DAAs assay make our detection platform highly promising for preliminary screening diagnosis of gastric cancer and patient self-detection applications. Our straightforward strategy for tuning POD-mimic activity provides an in-depth understanding on structure-activity relationship in nanozymes, offering valuable opportunities to advance enzyme-mimic optimization for other potential applications.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Real-time detecting of triethylamine gas is necessary and challenging in environmental protection and industrial production. However, conventional sensing materials still suffer from low response, long response times and high detection limit, mainly due to the insufficient charge transfer capability of the sensing materials. Herein, ZnWO4/WO3 was successfully prepared for TEA detection by a two-step hydrothermal method. Benefiting from the synergy between the heterojunctions, including electron sensitization and chemical sensitization, the sensor based on 0.1-ZnWO4/WO3 composite demonstrates remarkable performance in terms of faster response/recovery time (3.3-fold/2.02-fold), higher response (2.21-fold), lower detection limit (0.5 ppm) and lower power consumption (30℃-decrement) as compared with the pristine WO3 sensor. In addition, the long-term stability, repeatability, 10 s fast response, and satisfactory anti-interference ability of the composite sensor indicate its potential application in TEA detection. This work provides a feasible scheme to design advanced gas sensors through the synergistic effect of electron sensitization and chemical sensitization.
{"title":"Electron sensitization and chemical sensitization of ZnWO4/WO3 Nanorod heterojunctions for high performance triethylamine sensor","authors":"Zhong-Yuan Wu, Yu-Feng Liu, Cheng Zhang, Xiao-Hong Zheng","doi":"10.1016/j.snb.2024.136870","DOIUrl":"https://doi.org/10.1016/j.snb.2024.136870","url":null,"abstract":"Real-time detecting of triethylamine gas is necessary and challenging in environmental protection and industrial production. However, conventional sensing materials still suffer from low response, long response times and high detection limit, mainly due to the insufficient charge transfer capability of the sensing materials. Herein, ZnWO<sub>4</sub>/WO<sub>3</sub> was successfully prepared for TEA detection by a two-step hydrothermal method. Benefiting from the synergy between the heterojunctions, including electron sensitization and chemical sensitization, the sensor based on 0.1-ZnWO<sub>4</sub>/WO<sub>3</sub> composite demonstrates remarkable performance in terms of faster response/recovery time (3.3-fold/2.02-fold), higher response (2.21-fold), lower detection limit (0.5 ppm) and lower power consumption (30℃-decrement) as compared with the pristine WO<sub>3</sub> sensor. In addition, the long-term stability, repeatability, 10<!-- --> <!-- -->s fast response, and satisfactory anti-interference ability of the composite sensor indicate its potential application in TEA detection. This work provides a feasible scheme to design advanced gas sensors through the synergistic effect of electron sensitization and chemical sensitization.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-31DOI: 10.1016/j.snb.2024.136873
Pan Yue, Li Ruiyi, Wang Xie, Shen Yirui, Li Zaijun
Existing electrochemical sensors are inadequate for detecting pesticides at extremely low level. This study presents an electrochemical biosensor based on gold-iridium alloy (Au5Ir) and DNA walker for detection of atrazine. Au5Ir@RFBP-GQD nanocomposite was synthesized via the reduction of chloroauric acid and iridium trichloride using arginine and folic acid-functionalized boron and phosphorus-doped graphene quantum dot (RFBP-GQD). The synergy between Au and Ir, along with the formation of Schottky heterojunction, enhances the catalytic activity. The Au5Ir was covalently conjugated with hairpin DNA and thionine forming a redox probe that was used to construct the electrochemical biosensor for atrazine detection, coupled with a DNA walker mechanism. Atrazine triggers the DNA walker, leading to the introduction of multiple redox probes onto the gold electrode surface. The oxidation and reduction of thionine molecules within these redox probes elicit highly sensitive electrochemical response. The integration of Au5Ir@RFBP-GQD catalysis with the DNA walker results in significant signal amplification. The biosensor exhibits superior sensitivity and selectivity compared to other atrazine sensors, with a linear detection range from 1×10-18 to 1×10-12 M and a low detection limit of 3.4×10-19 M (S/N=3). The proposed analytical method was successfully used for detection of atrazine in environmental water.
现有的电化学传感器不足以检测极低浓度的农药。本研究提出了一种基于金铱合金（Au5Ir）和 DNA walker 的电化学生物传感器，用于检测阿特拉津。Au5Ir@RFBP-GQD 纳米复合材料是通过精氨酸和叶酸官能化硼磷掺杂石墨烯量子点（RFBP-GQD）还原氯金酸和三氯化铱合成的。金和铱之间的协同作用以及肖特基异质结的形成增强了催化活性。Au5Ir 与发夹 DNA 和亚硫酸共价共轭，形成氧化还原探针，用于构建检测阿特拉津的电化学生物传感器，并与 DNA walker 机制相结合。阿特拉津触发了 DNA 步行器，从而将多个氧化还原探针引入金电极表面。这些氧化还原探针中的硫氨酸分子的氧化和还原作用会引起高灵敏度的电化学反应。Au5Ir@RFBP-GQD 催化与 DNA 步行器的整合可显著放大信号。与其他阿特拉津传感器相比，该生物传感器具有更高的灵敏度和选择性，线性检测范围为 1×10-18 至 1×10-12 M，检测限低至 3.4×10-19 M（S/N=3）。所提出的分析方法被成功用于环境水体中莠去津的检测。
{"title":"Electrochemical biosensor based on Au5Ir@graphene quantum dot nanocomposite and DNA walker for detection of atrazine in environmental water with extremely high sensitivity and selectivity","authors":"Pan Yue, Li Ruiyi, Wang Xie, Shen Yirui, Li Zaijun","doi":"10.1016/j.snb.2024.136873","DOIUrl":"https://doi.org/10.1016/j.snb.2024.136873","url":null,"abstract":"Existing electrochemical sensors are inadequate for detecting pesticides at extremely low level. This study presents an electrochemical biosensor based on gold-iridium alloy (Au<sub>5</sub>Ir) and DNA walker for detection of atrazine. Au<sub>5</sub>Ir@RFBP-GQD nanocomposite was synthesized <em>via</em> the reduction of chloroauric acid and iridium trichloride using arginine and folic acid-functionalized boron and phosphorus-doped graphene quantum dot (RFBP-GQD). The synergy between Au and Ir, along with the formation of Schottky heterojunction, enhances the catalytic activity. The Au<sub>5</sub>Ir was covalently conjugated with hairpin DNA and thionine forming a redox probe that was used to construct the electrochemical biosensor for atrazine detection, coupled with a DNA walker mechanism. Atrazine triggers the DNA walker, leading to the introduction of multiple redox probes onto the gold electrode surface. The oxidation and reduction of thionine molecules within these redox probes elicit highly sensitive electrochemical response. The integration of Au<sub>5</sub>Ir@RFBP-GQD catalysis with the DNA walker results in significant signal amplification. The biosensor exhibits superior sensitivity and selectivity compared to other atrazine sensors, with a linear detection range from 1×10<sup>-18</sup> to 1×10<sup>-12<!-- --> </sup>M and a low detection limit of 3.4×10<sup>-19<!-- --> </sup>M (S/N=3). The proposed analytical method was successfully used for detection of atrazine in environmental water.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142562153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Electrochemiluminescence (ECL) initiated precisely by potential can effectively control the timing and increase the intensity of chemiluminescence, making it a vital light source for optical biosensors. Chemiluminescence resonance energy transfer (CRET) involves non-radiative energy transfer from a chemiluminescent donor to an appropriate acceptor molecule, eliminating the need for a laser light and offering a low background. Magnetic carbon nanotubes (MCNTs) possess magnetic responsiveness, high conductivity, and ease of modification. We designed and synthesized multifunctional magnetic carriers to capture miRNA targets (miRNA-183 or miRNA-21). In the presence of luminol, peroxidase catalyzes the ECL reaction at an oxidative potential of 250 mV, transferring 425 nm energy to excite two organic fluorescent dyes (Pacific Blue and Alexa fluor) on the detection probes. These dyes emit distinct fluorescence, allowing for the simultaneous detection of two miRNAs. This simple and controllable ECL design, combined with functionalized MCNTs and a high-efficiency CRET mechanism, enables simultaneous dual-miRNA detection within 30 minutes. The detection limits for miRNA-21 and miRNA-183 in human serum are 0.38 fM and 0.49 fM, respectively. The developed ECL detection system demonstrates high energy transfer efficiency, reasonable specificity, and selectivity, which provides a promising approach for rapid multi-target detection in on-site diagnostics.
{"title":"Sensitive dual-target electrochemiluminescence biosensor for simultaneous miRNA detection using magnetic carbon nanotubes and resonance energy transfer","authors":"Chi-Hsien Liu, Pravanjan Malla, Wei-Chi Wu, Selvaganapathy Ganesan","doi":"10.1016/j.snb.2024.136876","DOIUrl":"https://doi.org/10.1016/j.snb.2024.136876","url":null,"abstract":"Electrochemiluminescence (ECL) initiated precisely by potential can effectively control the timing and increase the intensity of chemiluminescence, making it a vital light source for optical biosensors. Chemiluminescence resonance energy transfer (CRET) involves non-radiative energy transfer from a chemiluminescent donor to an appropriate acceptor molecule, eliminating the need for a laser light and offering a low background. Magnetic carbon nanotubes (MCNTs) possess magnetic responsiveness, high conductivity, and ease of modification. We designed and synthesized multifunctional magnetic carriers to capture miRNA targets (miRNA-183 or miRNA-21). In the presence of luminol, peroxidase catalyzes the ECL reaction at an oxidative potential of 250<!-- --> <!-- -->mV, transferring 425<!-- --> <!-- -->nm energy to excite two organic fluorescent dyes (Pacific Blue and Alexa fluor) on the detection probes. These dyes emit distinct fluorescence, allowing for the simultaneous detection of two miRNAs. This simple and controllable ECL design, combined with functionalized MCNTs and a high-efficiency CRET mechanism, enables simultaneous dual-miRNA detection within 30<!-- --> <!-- -->minutes. The detection limits for miRNA-21 and miRNA-183 in human serum are 0.38 fM and 0.49 fM, respectively. The developed ECL detection system demonstrates high energy transfer efficiency, reasonable specificity, and selectivity, which provides a promising approach for rapid multi-target detection in on-site diagnostics.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":null,"pages":null},"PeriodicalIF":8.4,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-30DOI: 10.1016/j.snb.2024.136869
The gas signaling molecules CO and Al3+ play important physiological roles in maintaining homeostatic processes in cells. Rapid monitoring of these two information molecules in the sub-organelle dimension has rarely been reported. To fill this blank, we proposed a new “molecular weight regulated FRET method” to construct new logic gate probes to monitor CO and Al3+ synergetically. We selected naphthalimide and rhodamine as energy transfer groups. Then we designed two silane probes, P-Si-CO and Si-CO, which display different chain lengths to investigate the probes' response to CO and Al3+. Si-CO with low molecular weight is more advantageous for generating significant FRET processes. To show the three-channel and dual detection function of the probe more intuitively, we constructed a novel logic gate model that can detect Al3+ and CO simultaneously. Si-CO also exhibited rewarding cell imaging effects for visualizing the anoxia and starvation process in lysosomes. This work provided a new method for constructing logic-gate probes for detecting Al3+ and CO based on regulating FRET theory.
气体信号分子 CO 和 Al3+ 在维持细胞平衡过程中发挥着重要的生理作用。在亚细胞器维度快速监测这两种信息分子的研究还鲜有报道。为了填补这一空白，我们提出了一种新的 "分子量调控 FRET 法"，以构建新的逻辑门探针来协同监测 CO 和 Al3+。我们选择了萘二甲酰亚胺和罗丹明作为能量转移基团。然后，我们设计了两种不同链长的硅烷探针--P-Si-CO 和 Si-CO，以研究探针对 CO 和 Al3+ 的响应。低分子量的 Si-CO 更有利于产生显著的 FRET 过程。为了更直观地展示探针的三通道和双检测功能，我们构建了一个新颖的逻辑门模型，可以同时检测 Al3+ 和 CO。此外，Si-CO 还在可视化溶酶体缺氧和饥饿过程方面表现出令人满意的细胞成像效果。这项工作为基于调控 FRET 理论构建检测 Al3+ 和 CO 的逻辑门探针提供了一种新方法。
{"title":"Molecular weight modulated FRET based logic gate probes for lysosome Al3+ and CO detection","authors":"","doi":"10.1016/j.snb.2024.136869","DOIUrl":"10.1016/j.snb.2024.136869","url":null,"abstract":"<div><div>The gas signaling molecules CO and Al<sup>3+</sup> play important physiological roles in maintaining homeostatic processes in cells. Rapid monitoring of these two information molecules in the sub-organelle dimension has rarely been reported. To fill this blank, we proposed a new “molecular weight regulated FRET method” to construct new logic gate probes to monitor CO and Al<sup>3+</sup> synergetically. We selected naphthalimide and rhodamine as energy transfer groups. Then we designed two silane probes, <strong>P-Si-CO</strong> and <strong>Si-CO</strong>, which display different chain lengths to investigate the probes' response to CO and Al<sup>3+</sup>. <strong>Si-CO</strong> with low molecular weight is more advantageous for generating significant FRET processes. To show the three-channel and dual detection function of the probe more intuitively, we constructed a novel logic gate model that can detect Al<sup>3+</sup> and CO simultaneously. <strong>Si-CO</strong> also exhibited rewarding cell imaging effects for visualizing the anoxia and starvation process in lysosomes. This work provided a new method for constructing logic-gate probes for detecting Al<sup>3+</sup> and CO based on regulating FRET theory.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":null,"pages":null},"PeriodicalIF":8.0,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142541861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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