Pub Date : 2025-02-13DOI: 10.1016/j.snb.2025.137442
Xiaojing Chen, Quan Tao, Siwei Yang, Hang Wang, Xuelian Wang, Guqiao Ding, Xiumin Gao, Hui Dong, Liangliang Rong
Aging allows the occurrence and progression of cerebrovascular diseases, highlighting the importance of detecting senescent cells and tissues in the nervous system. Detecting senescent cells/tissues using nanoparticles as probes in the nervous system is desired with high sensitivity, high accuracy, and penetration of the blood-brain barrier. In response, we designed crown ether-based graphene quantum dots with fast proton transport channels to sense potassium ions, a significant differential indicator between healthy and senescent cells. Using the probe, we have achieved MRI-fluorescence dual-functional monitoring of potassium ion concentration in cells via fluorescent intensity and magnetic relaxation time. Importantly, we have further demonstrated the advantages of the crown ether-based graphene quantum dots in penetrating the blood-brain barrier, revealing their potential for diagnosing aging in the nervous system.
{"title":"K+ concentration-based NMR-fluorescence dual-functional senescence sensing using graphene quantum dots with crown ether structure","authors":"Xiaojing Chen, Quan Tao, Siwei Yang, Hang Wang, Xuelian Wang, Guqiao Ding, Xiumin Gao, Hui Dong, Liangliang Rong","doi":"10.1016/j.snb.2025.137442","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137442","url":null,"abstract":"Aging allows the occurrence and progression of cerebrovascular diseases, highlighting the importance of detecting senescent cells and tissues in the nervous system. Detecting senescent cells/tissues using nanoparticles as probes in the nervous system is desired with high sensitivity, high accuracy, and penetration of the blood-brain barrier. In response, we designed crown ether-based graphene quantum dots with fast proton transport channels to sense potassium ions, a significant differential indicator between healthy and senescent cells. Using the probe, we have achieved MRI-fluorescence dual-functional monitoring of potassium ion concentration in cells <em>via</em> fluorescent intensity and magnetic relaxation time. Importantly, we have further demonstrated the advantages of the crown ether-based graphene quantum dots in penetrating the blood-brain barrier, revealing their potential for diagnosing aging in the nervous system.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"32 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143401975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-13DOI: 10.1016/j.snb.2025.137431
Li-Ping Fan, Xing-Miao Wang, Juan Li, Tong Shao, Ya-Xin Wang, De-Ming Kong
Fat mass and obesity-associated protein (FTO) plays a crucial role in various physiological processes by dynamically regulating mRNA expression through the control of N6-methyladenosine (m6A) levels. Dysfunctions in FTO lead to numerous human diseases, attracting significant attention in biology and medicine. Herein, we have developed a ratiometric fluorescence biosensor based on demethylase-activated deoxyribozyme (DNAzyme) triggered three-dimensional DNA nanoamplifiers (TDNs) assisted by magnetic beads (MBs). Specifically, we engineered an m6A-silenced DNAzyme as the biosensor switch that can be specifically activated by the demethylation activity of FTO. The activated DNAzyme catalyzes the cleavage of substrates on MBs, generating multiple primers that can induce a DNA tetrahedron-mediated hyperbranched hybridization chain reaction (TD-HCR) after magnetic separation, resulting in the formation of hyperbranched products with greatly enhanced fluorescence resonance energy transfer (FRET) signals. Our approach allows for sensitive and quantitative detection of FTO with an impressive limit of detection (LOD) as low as 2.38 fM, surpassing the sensitivity of conventional ELISA kits and mostly reported methods. Furthermore, the proposed strategy enables effectively distinguishing the expression levels of FTO in tissues derived from both healthy individuals and breast cancer patients, thus offering an innovative foundation for drug discovery endeavors, investigations into m6A modifications, and advancements in clinical diagnostic capabilities.
{"title":"Three-dimensional DNA nanoamplifiers actuated by demethylase-activated deoxyribozyme for the ultrasensitive detection of FTO in human breast tissues","authors":"Li-Ping Fan, Xing-Miao Wang, Juan Li, Tong Shao, Ya-Xin Wang, De-Ming Kong","doi":"10.1016/j.snb.2025.137431","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137431","url":null,"abstract":"Fat mass and obesity-associated protein (FTO) plays a crucial role in various physiological processes by dynamically regulating mRNA expression through the control of N6-methyladenosine (m<sup>6</sup>A) levels. Dysfunctions in FTO lead to numerous human diseases, attracting significant attention in biology and medicine. Herein, we have developed a ratiometric fluorescence biosensor based on demethylase-activated deoxyribozyme (DNAzyme) triggered three-dimensional DNA nanoamplifiers (TDNs) assisted by magnetic beads (MBs). Specifically, we engineered an m<sup>6</sup>A-silenced DNAzyme as the biosensor switch that can be specifically activated by the demethylation activity of FTO. The activated DNAzyme catalyzes the cleavage of substrates on MBs, generating multiple primers that can induce a DNA tetrahedron-mediated hyperbranched hybridization chain reaction (TD-HCR) after magnetic separation, resulting in the formation of hyperbranched products with greatly enhanced fluorescence resonance energy transfer (FRET) signals. Our approach allows for sensitive and quantitative detection of FTO with an impressive limit of detection (LOD) as low as 2.38 fM, surpassing the sensitivity of conventional ELISA kits and mostly reported methods. Furthermore, the proposed strategy enables effectively distinguishing the expression levels of FTO in tissues derived from both healthy individuals and breast cancer patients, thus offering an innovative foundation for drug discovery endeavors, investigations into m<sup>6</sup>A modifications, and advancements in clinical diagnostic capabilities.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"10 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143401973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-12DOI: 10.1016/j.snb.2025.137441
Dario Angelone, Liang Li, Ciaran Devoy, Andrew B. Matheson, Ahmet T. Erdogan, Robert K. Henderson, Mark Tangney, Dmitri B. Papkovsky, Stefan Andersson-Engels, Sanathana Konugolu Venkata Sekar
Extracellular pH (pHe) is an important indicator of the homeostatic condition of mammalian tissue and its pathophysiological state such as tumor development and tissue acidosis. Optochemical sensors with internal referencing capabilities can perform dynamic quantitative imaging of pHe in live tissue samples. Here we have used planar solid-state pH sensors based on a fluorescent octaethylporphin-ketone (OEPK) dye in plasticized PVC matrix, which produce robust fluorescence lifetime response in the physiological pH range and stable lifetime calibration. A custom-built fluorescence lifetime imager, with a 405 nm picosecond diode laser excitation and a photon counting SPAD array detector (120×128 pixels) with wide-field Fluorescence Lifetime Imaging (FLIM) capability, was used to image the planar pH sensor brought in contact with freshly excised normal and tumor tissue. The FLIM based sensor system has demonstrated good analytical performance and suitability for in vivo use. Producing detailed maps of tissue pHe, such sensors can facilitate a deeper understanding of tissue metabolism and physiology, and can find use in fluorescence guided surgery of solid tumors.
{"title":"Metabolic imaging of tumor tissue samples using planar fluorescence lifetime-based pH sensors and a SPAD array imager","authors":"Dario Angelone, Liang Li, Ciaran Devoy, Andrew B. Matheson, Ahmet T. Erdogan, Robert K. Henderson, Mark Tangney, Dmitri B. Papkovsky, Stefan Andersson-Engels, Sanathana Konugolu Venkata Sekar","doi":"10.1016/j.snb.2025.137441","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137441","url":null,"abstract":"Extracellular pH (pHe) is an important indicator of the homeostatic condition of mammalian tissue and its pathophysiological state such as tumor development and tissue acidosis. Optochemical sensors with internal referencing capabilities can perform dynamic quantitative imaging of pHe in live tissue samples. Here we have used planar solid-state pH sensors based on a fluorescent octaethylporphin-ketone (OEPK) dye in plasticized PVC matrix, which produce robust fluorescence lifetime response in the physiological pH range and stable lifetime calibration. A custom-built fluorescence lifetime imager, with a 405<!-- --> <!-- -->nm picosecond diode laser excitation and a photon counting SPAD array detector (120×128 pixels) with wide-field Fluorescence Lifetime Imaging (FLIM) capability, was used to image the planar pH sensor brought in contact with freshly excised normal and tumor tissue. The FLIM based sensor system has demonstrated good analytical performance and suitability for <em>in vivo</em> use. Producing detailed maps of tissue pHe, such sensors can facilitate a deeper understanding of tissue metabolism and physiology, and can find use in fluorescence guided surgery of solid tumors.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"15 1 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143401958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Finding a simple way to improve the performance of Ti3C2Tx humidity sensors is a huge challenge. Unlike the complex preparation process in the past, we reported on different levels of humidity sensors that can be obtained during the oxidation process of Few-layer Ti3C2Tx without artificial intervention. The Ti3C2Tx content m in the oxidation products of anatase TiO2/mTi3C2Tx could be readily optimized by monitoring the storage time. A small portion of the surface of Ti3C2Tx was transformed into rutile TiO2 by ultraviolet ozone (UV) irradiation, and a rutile-anatase TiO2/mTi3C2Tx triple junction material is obtained. The corresponding sensor exhibits different levels of sensing performance. In addition, a new sensing mechanism that gradually degrades from inductance characteristics to capacitive characteristics has been revealed. The closed-loop recycling mode without material loss designed in this work also is expected to solve the reuse problem of Mxene waste after oxidation.
{"title":"Closed loop acquisition of multi-levels humidity sensors by spontaneous optimization TiO2/Ti3C2Tx heterojunction without artificial intervention","authors":"Zhuoyu Yang, Weiqing Liu, Ligang Yuan, Wenhao Chen, Gaigai Ma, Jiayi Xu, Qiang Wu","doi":"10.1016/j.snb.2025.137426","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137426","url":null,"abstract":"Finding a simple way to improve the performance of Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> humidity sensors is a huge challenge. Unlike the complex preparation process in the past, we reported on different levels of humidity sensors that can be obtained during the oxidation process of Few-layer Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> without artificial intervention. The Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> content <em>m</em> in the oxidation products of anatase TiO<sub>2</sub>/<em>m</em>Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> could be readily optimized by monitoring the storage time. A small portion of the surface of Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> was transformed into rutile TiO<sub>2</sub> by ultraviolet ozone (UV) irradiation, and a rutile-anatase TiO<sub>2</sub>/<em>m</em>Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> triple junction material is obtained. The corresponding sensor exhibits different levels of sensing performance. In addition, a new sensing mechanism that gradually degrades from inductance characteristics to capacitive characteristics has been revealed. The closed-loop recycling mode without material loss designed in this work also is expected to solve the reuse problem of Mxene waste after oxidation.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"40 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143393881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-12DOI: 10.1016/j.snb.2025.137423
Ying Qian, Jie Zou, Xiaoqing Jiang, Jie Wang, Juan Zhou, Chu Cheng, Xin Zhang, Wenming He, Qinghui Jin, Jiawen Jian
Yttria-stabilized zirconia (YSZ)-based amperometric humidity sensors are highly promising for in situ detection of high-temperature flue ducts due to their excellent stability and durability under extreme conditions. However, selectivity remains challenging due to the co-electrolysis of H2O and CO2, as these compounds have similar electrolysis voltages. In this study, LaFeO3 is proposed as a sensing electrode to reduce CO2 electrolysis and improve selectivity in amperometric humidity sensors. Analysis indicates that the improved selectivity is likely due to LaFeO3’s lower electrochemical activity. Furthermore, the proposed sensor exhibits good repeatability and acceptable long-term stability for high-temperature humidity detection.
{"title":"Improving CO2 resistance in high-temperature humidity sensors using LaFeO3 sensing electrodes","authors":"Ying Qian, Jie Zou, Xiaoqing Jiang, Jie Wang, Juan Zhou, Chu Cheng, Xin Zhang, Wenming He, Qinghui Jin, Jiawen Jian","doi":"10.1016/j.snb.2025.137423","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137423","url":null,"abstract":"Yttria-stabilized zirconia (YSZ)-based amperometric humidity sensors are highly promising for in situ detection of high-temperature flue ducts due to their excellent stability and durability under extreme conditions. However, selectivity remains challenging due to the co-electrolysis of H<sub>2</sub>O and CO<sub>2</sub>, as these compounds have similar electrolysis voltages. In this study, LaFeO<sub>3</sub> is proposed as a sensing electrode to reduce CO<sub>2</sub> electrolysis and improve selectivity in amperometric humidity sensors. Analysis indicates that the improved selectivity is likely due to LaFeO<sub>3</sub>’s lower electrochemical activity. Furthermore, the proposed sensor exhibits good repeatability and acceptable long-term stability for high-temperature humidity detection.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"3 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143393882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acetone is a crucial biomarker of diabetes diagnosis by breath analysis. However, the fabrication of metal oxide semiconductor-based sensors with the desired gas-sensing abilities for sub-ppm acetone detection remains a significant challenge. In this study, heterostructured NixFe3-xO4 composites with varying Ni concentrations were effectively developed using a simple one-step solvothermal process followed by post-calcination treatment. The sensor based on NixFe3-xO4 composites exhibited excellent gas sensing performance toward 50 ppm acetone due to the formation of unique microstructures and numerous active sites. Notably, the optimum Ni0.8Fe2.2O4 sensor demonstrated remarkable sensitivity even at sub-ppm levels, showing good monotonically increasing response (R2 = 0.994) within the concentration range of 0.1-5.0 ppm acetone. Moreover, distinct signal responses between exhaled breath samples from a healthy individual and a simulated diabetic patient were observed in the Ni0.8Fe2.2O4 sensor, implying that the NixFe3-xO4 composites provided an alternative novel strategy for the effective detection of acetone in actual breath analysis of diabetes diagnosis.
{"title":"Heterostructured NixFe3-xO4 composites for sub-ppm acetone detection","authors":"Xiangjian Liao, Xingyu Liu, Yilu Wu, Shufen Li, Guojiao Ren, Yuxiang Ou, Shiquan Chen, Zixuan Wu, Aiping Huang, Xianzhe Liu, Jianyi Luo","doi":"10.1016/j.snb.2025.137425","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137425","url":null,"abstract":"Acetone is a crucial biomarker of diabetes diagnosis by breath analysis. However, the fabrication of metal oxide semiconductor-based sensors with the desired gas-sensing abilities for sub-ppm acetone detection remains a significant challenge. In this study, heterostructured Ni<sub>x</sub>Fe<sub>3-x</sub>O<sub>4</sub> composites with varying Ni concentrations were effectively developed using a simple one-step solvothermal process followed by post-calcination treatment. The sensor based on Ni<sub>x</sub>Fe<sub>3-x</sub>O<sub>4</sub> composites exhibited excellent gas sensing performance toward 50 ppm acetone due to the formation of unique microstructures and numerous active sites. Notably, the optimum Ni<sub>0.8</sub>Fe<sub>2.2</sub>O<sub>4</sub> sensor demonstrated remarkable sensitivity even at sub-ppm levels, showing good monotonically increasing response (R<sup>2</sup> = 0.994) within the concentration range of 0.1-5.0 ppm acetone. Moreover, distinct signal responses between exhaled breath samples from a healthy individual and a simulated diabetic patient were observed in the Ni<sub>0.8</sub>Fe<sub>2.2</sub>O<sub>4</sub> sensor, implying that the Ni<sub>x</sub>Fe<sub>3-x</sub>O<sub>4</sub> composites provided an alternative novel strategy for the effective detection of acetone in actual breath analysis of diabetes diagnosis.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"7 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143393923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-12DOI: 10.1016/j.snb.2025.137428
G. Oliva, L. Manin, S. Valić, S.K. Islam, A.S. Fiorillo, S.A. Pullano
Tomato seed oil (TSO) is an edible product characterized by a wide range of molecules, with beneficial effects on human health. Volatile organic compounds (VOC) formed from the degradation of fatty acids, are promising candidates for the characterization of vegetable oils. Hereafter, a sensor based on photoionization detector for palmitic acid is presented. The sensor exploits thermal emission profile analysis from a thin layer of zeolite 5 A. Emissive profiles were acquired through a Photoionization Detector (PID) at 100 °C. Specifically, the combination of use of zeolite with pore size of 5.1 Å and an ultraviolet lamp of 10.9 eV allows selective adsorption and detection of palmitic acid in a solution of pentane and TSO. The PID-zeolite sensor was investigated using oils at different dilution and at different storage conditions (−20 °C, 4 °C and 25 °C). Results evidenced that pentane dilution plays a significant role in palmitic acid adsorption, with a maximum emissive profile at ~885 µmol/L. Low temperature storage (−20 °C) of samples before analysis results in 1.5 times higher emission peak due to the formation of triple chain molecular arrangement of palmitic acid. Calibration evidenced a linear range from 0.45 mmol/L up to 1.8 mmol/L with a sensitivity of 34.65 ppm∙mmol−1∙L and an R2 = 0.92. Real scenario analysis was performed a mixture of TSO with soybean oil (SO) at different storage stability. A significant emissive reduction in palmitic acid was observed in mixed oil, depending on its stability which allows for the evaluation of adulterated samples.
{"title":"Zeolite 5 A Mediated Palmitic Acid Detection in Tomato Seed Oil by Photoionization Detector","authors":"G. Oliva, L. Manin, S. Valić, S.K. Islam, A.S. Fiorillo, S.A. Pullano","doi":"10.1016/j.snb.2025.137428","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137428","url":null,"abstract":"Tomato seed oil (TSO) is an edible product characterized by a wide range of molecules, with beneficial effects on human health. Volatile organic compounds (VOC) formed from the degradation of fatty acids, are promising candidates for the characterization of vegetable oils. Hereafter, a sensor based on photoionization detector for palmitic acid is presented. The sensor exploits thermal emission profile analysis from a thin layer of zeolite 5<!-- --> <!-- -->A. Emissive profiles were acquired through a Photoionization Detector (PID) at 100 °C. Specifically, the combination of use of zeolite with pore size of 5.1<!-- --> <!-- -->Å and an ultraviolet lamp of 10.9<!-- --> <!-- -->eV allows selective adsorption and detection of palmitic acid in a solution of pentane and TSO. The PID-zeolite sensor was investigated using oils at different dilution and at different storage conditions (−20 °C, 4 °C and 25 °C). Results evidenced that pentane dilution plays a significant role in palmitic acid adsorption, with a maximum emissive profile at ~885 µmol/L. Low temperature storage (−20 °C) of samples before analysis results in 1.5 times higher emission peak due to the formation of triple chain molecular arrangement of palmitic acid. Calibration evidenced a linear range from 0.45<!-- --> <!-- -->mmol/L up to 1.8<!-- --> <!-- -->mmol/L with a sensitivity of 34.65 ppm∙mmol<sup>−1</sup>∙L and an R<sup>2</sup> = 0.92. Real scenario analysis was performed a mixture of TSO with soybean oil (SO) at different storage stability. A significant emissive reduction in palmitic acid was observed in mixed oil, depending on its stability which allows for the evaluation of adulterated samples.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"59 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143401976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Notum, an important member of the serine hydrolase superfamily, acts as a key negative feedback regulator of the Wnt signaling. Inhibiting Notum activity has emerged as a feasible therapeutic strategy for treating osteoporosis (OP) via activating Wnt/β-catenin signaling. This work aimed to develop a high-performance near-infrared (NIR) fluorogenic sensor for high-throughput screening and characterization of anti-Notum agents. First, a dual-driven strategy combining computer-aided molecular design with enzymatic phenotyping assays was utilized to identify an NIR fluorogenic scaffold (HTCF) for engineering Notum substrates. A suite of HTCF esters were then synthesized, while HX-HTCF (the n-hexanoate ester of HTCF) was selected as an optimum substrate for Notum owing to its desirable NIR emission, rapid response, superior signal-to-noise ratio, ultrahigh sensitivity, and high affinity. HX-HTCF was subsequently used to construct a fluorescence-based biochemical assay for the highly efficient discovery of Notum inhibitors. H5 was identified as a novel potent Notum inhibitor, which strongly inhibited Notum-catalyzed HX-HTCF hydrolysis in a competitive manner. Further investigations showed that H5 significantly activated Wnt/β-catenin signaling and promoted osteoblast differentiation in dexamethasone-induced MC3T3-E1 osteoblasts. Collectively, this study devises a practical Notum-activatable NIR fluorogenic sensor, which immensely facilitates the discovery of Notum inhibitors as novel anti-osteoporosis agents.
{"title":"Rational engineering of an optimized near-infrared fluorogenic sensor for efficient discovery of potent Notum inhibitors as anti-osteoporosis agents","authors":"Lele Liu, Yufan Fan, Hong Lin, Jingxuan Lei, Jia Guo, Lin Chen, Zhangping Xiao, Yunqing Song, Xiaodi Yang, Guangbo Ge, Hui Tang","doi":"10.1016/j.snb.2025.137427","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137427","url":null,"abstract":"Notum, an important member of the serine hydrolase superfamily, acts as a key negative feedback regulator of the Wnt signaling. Inhibiting Notum activity has emerged as a feasible therapeutic strategy for treating osteoporosis (OP) <em>via</em> activating Wnt/β-catenin signaling. This work aimed to develop a high-performance near-infrared (NIR) fluorogenic sensor for high-throughput screening and characterization of anti-Notum agents. First, a dual-driven strategy combining computer-aided molecular design with enzymatic phenotyping assays was utilized to identify an NIR fluorogenic scaffold (<strong>HTCF</strong>) for engineering Notum substrates. A suite of <strong>HTCF</strong> esters were then synthesized, while <strong>HX-HTCF</strong> (the <em>n</em>-hexanoate ester of <strong>HTCF</strong>) was selected as an optimum substrate for Notum owing to its desirable NIR emission, rapid response, superior signal-to-noise ratio, ultrahigh sensitivity, and high affinity. <strong>HX-HTCF</strong> was subsequently used to construct a fluorescence-based biochemical assay for the highly efficient discovery of Notum inhibitors. <strong>H5</strong> was identified as a novel potent Notum inhibitor, which strongly inhibited Notum-catalyzed <strong>HX-HTCF</strong> hydrolysis in a competitive manner. Further investigations showed that <strong>H5</strong> significantly activated Wnt/β-catenin signaling and promoted osteoblast differentiation in dexamethasone-induced MC3T3-E1 osteoblasts. Collectively, this study devises a practical Notum-activatable NIR fluorogenic sensor, which immensely facilitates the discovery of Notum inhibitors as novel anti-osteoporosis agents.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"15 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143393880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, we constructed a dual-mode electrochemical-electrochemiluminescent (EC-ECL) sensor based on a hydrogen-bonded organic framework (RuHOFs) with Ru(dcbpy)32+) as the primary emitter and [2,2'-bipyridine]-5,5'-diamine (DAP) as the secondary ligand. This sensor enables efficient and sensitive detection of heavy metal ions (HMIs), mercury ions (Hg²⁺) and zinc ions (Zn²⁺). RuHOFs possess a high specific surface area and a stable hydrogen-bond network, offering ideal N,N'-bipyridine chelating sites for effective recognition of metal ions, significantly enhancing the stability of both EC and ECL signals. The results showed that the sensor produced only a rising ECL signal with a limit of detection (LOD) of 13 pM (1 μM~10 pM) after interacting with Zn²⁺, and upon binding with Hg²⁺, a declining ECL signal and an amplification of the DPV signal were detected, the LOD of its ECL and EC reached 0.55 pM (0.1 μM~1 pM) and 0.26 nM (1 μM~5 nM), respectively. Moreover, by growing RuHOFs on the surface of carbon fibers, a RuHOFs-modified carbon fiber microelectrode (CFM) electrochemical sensor was prepared, achieving a significant enhancement in sensitivity for Hg²⁺ detection, with an LOD of 0.09 nM (0.05 μM ~ 0.1 nM), and meanwhile, the sensor was effectively utilized for the electrochemical detection of Hg²⁺ in rat blood. The synthesis of RuHOFs presents new potential for the construction of EC-ECL sensing platforms, and new research ideas and prospective applications in the areas of environmental monitoring and biosensing.
{"title":"A Dual-Mode Electrochemical and Electrochemiluminescent Sensor Based on Hydrogen-Bonded Organic Frameworks for Highly Sensitive Detection of Mercury and Zinc Ions","authors":"Xinying Yan, Zhaojiang Yin, Yanqing Xu, Haotian Xie, Huiting Hu, Hao Fan, Jing Zhang","doi":"10.1016/j.snb.2025.137433","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137433","url":null,"abstract":"In this study, we constructed a dual-mode electrochemical-electrochemiluminescent (EC-ECL) sensor based on a hydrogen-bonded organic framework (RuHOFs) with Ru(dcbpy)<sub>3</sub><sup>2+</sup>) as the primary emitter and [2,2'-bipyridine]-5,5'-diamine (DAP) as the secondary ligand. This sensor enables efficient and sensitive detection of heavy metal ions (HMIs), mercury ions (Hg²⁺) and zinc ions (Zn²⁺). RuHOFs possess a high specific surface area and a stable hydrogen-bond network, offering ideal N,N'-bipyridine chelating sites for effective recognition of metal ions, significantly enhancing the stability of both EC and ECL signals. The results showed that the sensor produced only a rising ECL signal with a limit of detection (LOD) of 13 pM (1<!-- --> <!-- -->μM~10 pM) after interacting with Zn²⁺, and upon binding with Hg²⁺, a declining ECL signal and an amplification of the DPV signal were detected, the LOD of its ECL and EC reached 0.55 pM (0.1<!-- --> <!-- -->μM~1 pM) and 0.26<!-- --> <!-- -->nM (1<!-- --> <!-- -->μM~5<!-- --> <!-- -->nM), respectively. Moreover, by growing RuHOFs on the surface of carbon fibers, a RuHOFs-modified carbon fiber microelectrode (CFM) electrochemical sensor was prepared, achieving a significant enhancement in sensitivity for Hg²⁺ detection, with an LOD of 0.09<!-- --> <!-- -->nM (0.05<!-- --> <!-- -->μM ~ 0.1<!-- --> <!-- -->nM), and meanwhile, the sensor was effectively utilized for the electrochemical detection of Hg²⁺ in rat blood. The synthesis of RuHOFs presents new potential for the construction of EC-ECL sensing platforms, and new research ideas and prospective applications in the areas of environmental monitoring and biosensing.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"87 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143401977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-11DOI: 10.1016/j.snb.2025.137424
Zhongyu Chen, Shaohai Fu, Yu Guan
In this study, we develop an optical liquid crystal (LC) sensor based on dodecylamine doped 4-cyano-4’-pentylbiphenyl (5CB) to accomplish real-time detection of formaldehyde (FA) vapor. When exposed to FA at ppm level, the LC sensor doped with 0.1 wt% dodecylamine shows a rapid dark-to-bright optical response within 10 s. The optical response of the LC sensor increases with the increasing concentration of FA vapor from 16.5 ppm to 824.1 ppm. This response is caused by the intermolecular force between dodecylamine and FA, which is verified by the Fourier transform infrared (FT-IR) spectrum. Meanwhile, the sensor demonstrates wide operational temperature range from 15℃ to 35℃, wide operational humidity range from 30% to 80% relative humidity (RH), high selectivity for FA vapor, excellent reversibility after 10 continuous tests, and reliable stability after storing at room temperature for 1 week. Overall, this LC sensor shows fast response, favorable specificity, excellent reversibility, and reliable stability, leading to a wider range of applications for FA detection.
{"title":"High-sensitive liquid crystal-based optical sensor for real-time detection of formaldehyde vapor","authors":"Zhongyu Chen, Shaohai Fu, Yu Guan","doi":"10.1016/j.snb.2025.137424","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137424","url":null,"abstract":"In this study, we develop an optical liquid crystal (LC) sensor based on dodecylamine doped 4-cyano-4’-pentylbiphenyl (5CB) to accomplish real-time detection of formaldehyde (FA) vapor. When exposed to FA at ppm level, the LC sensor doped with 0.1<!-- --> <!-- -->wt% dodecylamine shows a rapid dark-to-bright optical response within 10<!-- --> <!-- -->s. The optical response of the LC sensor increases with the increasing concentration of FA vapor from 16.5 ppm to 824.1 ppm. This response is caused by the intermolecular force between dodecylamine and FA, which is verified by the Fourier transform infrared (FT-IR) spectrum. Meanwhile, the sensor demonstrates wide operational temperature range from 15℃ to 35℃, wide operational humidity range from 30% to 80% relative humidity (RH), high selectivity for FA vapor, excellent reversibility after 10 continuous tests, and reliable stability after storing at room temperature for 1 week. Overall, this LC sensor shows fast response, favorable specificity, excellent reversibility, and reliable stability, leading to a wider range of applications for FA detection.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"86 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143385752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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