Pub Date : 2025-04-03DOI: 10.1016/j.snb.2025.137736
Xiaohong Su, Haotian Shan, Yanting Tian, Wei Guo, Peng Zhao, Lin Xue, Yongjia Zhang
This study investigates the enhancement of low-concentration CO2 sensing performance in Sr- and Co-doped LaFeO3 (LFO) thick film sensors through a combined treatment involving vacuum annealing and oxygen-rich atmosphere exposure. La0.8Sr0.2FeO3 (LSFO) and LaFe0.8Co0.2O3 (LFCO) porous microspheres were synthesized via hydrothermal method and characterized using XRD, SEM, XPS, and Raman spectroscopy. The optimized LFCO sensor demonstrated a high response value of 1.158 at 110°C, with rapid response/recovery times and excellent moisture resistance. The improved sensitivity was attributed to increased surface oxygen vacancies from vacuum annealing, promoting oxygen adsorption and electron transfer with CO2. First-principles calculations confirmed the role of O2 adsorption in enhancing the number of transferred electrons for CO2 molecules, thus improving gas sensing performance. This study presents a practical approach for developing cost-effective, high-performance CO2 sensors with enhanced sensitivity and stability.
{"title":"Enhanced low-concentration CO2 sensing performance for Sr- and Co-doped LaFeO3 thick film sensors by oxygen-enriched atmosphere after vacuum annealing","authors":"Xiaohong Su, Haotian Shan, Yanting Tian, Wei Guo, Peng Zhao, Lin Xue, Yongjia Zhang","doi":"10.1016/j.snb.2025.137736","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137736","url":null,"abstract":"This study investigates the enhancement of low-concentration CO<sub>2</sub> sensing performance in Sr- and Co-doped LaFeO<sub>3</sub> (LFO) thick film sensors through a combined treatment involving vacuum annealing and oxygen-rich atmosphere exposure. La<sub>0.8</sub>Sr<sub>0.2</sub>FeO<sub>3</sub> (LSFO) and LaFe<sub>0.8</sub>Co<sub>0.2</sub>O<sub>3</sub> (LFCO) porous microspheres were synthesized via hydrothermal method and characterized using XRD, SEM, XPS, and Raman spectroscopy. The optimized LFCO sensor demonstrated a high response value of 1.158 at 110°C, with rapid response/recovery times and excellent moisture resistance. The improved sensitivity was attributed to increased surface oxygen vacancies from vacuum annealing, promoting oxygen adsorption and electron transfer with CO<sub>2</sub>. First-principles calculations confirmed the role of O<sub>2</sub> adsorption in enhancing the number of transferred electrons for CO<sub>2</sub> molecules, thus improving gas sensing performance. This study presents a practical approach for developing cost-effective, high-performance CO<sub>2</sub> sensors with enhanced sensitivity and stability.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"73 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-03DOI: 10.1016/j.snb.2025.137732
Shumei Huang, Xiaomeng Du, Zhenxin Lin, Gang Chen, Yin Jiang, Wenhui You, Shen Zeng, Huatang Zhang
This study presents the development of a novel RNA-targeted fluorescent probe, O-698, for real-time imaging of RNA in living cells and tumor models. O-698 exhibits significant RNA sensitivity with low cytotoxicity and strong near-infrared (NIR) fluorescence. Subcellular localization studies demonstrated that O-698 selectively binds to RNA in nucleoli and cytoplasm, with minimal overlap with other organelle markers, and outperforms existing commercial RNA probes in terms of specificity and intensity. Furthermore, O-698 demonstrated exceptional stability in cells, maintaining fluorescence for over 8 hours, and was effective in monitoring RNA dynamics in response to RNA polymerase inhibition. Upon binding with RNA, O-698 offers a high signal-to-noise ratio for precise visualization without the need for wash steps. In 3D multicellular tumor spheroids, O-698 demonstrated robust penetration and uniform distribution throughout the tumor-like mass, reflecting its excellent capability to diffuse in dense biological tissues. In vivo imaging in tumor-bearing mice revealed strong fluorescence signals at the tumor injection site, consistent with the deep imaging capability of O-698. These findings underscore the potential of O-698 as a powerful tool for studying RNA dynamics in both cellular and in vivo environments. Overall, the rapid, stable, and wash-free imaging capabilities of O-698 make it a promising probe for real-time monitoring of RNA behavior, providing valuable insights into drug mechanisms and therapeutic efficacy.
{"title":"A Novel NIR Absorption and Emission Probe for Imaging of RNA Dynamics in Live Cells and Tumor Models","authors":"Shumei Huang, Xiaomeng Du, Zhenxin Lin, Gang Chen, Yin Jiang, Wenhui You, Shen Zeng, Huatang Zhang","doi":"10.1016/j.snb.2025.137732","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137732","url":null,"abstract":"This study presents the development of a novel RNA-targeted fluorescent probe, <strong>O-698</strong>, for real-time imaging of RNA in living cells and tumor models. <strong>O-698</strong> exhibits significant RNA sensitivity with low cytotoxicity and strong near-infrared (NIR) fluorescence. Subcellular localization studies demonstrated that <strong>O-698</strong> selectively binds to RNA in nucleoli and cytoplasm, with minimal overlap with other organelle markers, and outperforms existing commercial RNA probes in terms of specificity and intensity. Furthermore, <strong>O-698</strong> demonstrated exceptional stability in cells, maintaining fluorescence for over 8<!-- --> <!-- -->hours, and was effective in monitoring RNA dynamics in response to RNA polymerase inhibition. Upon binding with RNA, <strong>O-698</strong> offers a high signal-to-noise ratio for precise visualization without the need for wash steps. In 3D multicellular tumor spheroids, <strong>O-698</strong> demonstrated robust penetration and uniform distribution throughout the tumor-like mass, reflecting its excellent capability to diffuse in dense biological tissues. In vivo imaging in tumor-bearing mice revealed strong fluorescence signals at the tumor injection site, consistent with the deep imaging capability of <strong>O-698</strong>. These findings underscore the potential of <strong>O-698</strong> as a powerful tool for studying RNA dynamics in both cellular and in vivo environments. Overall, the rapid, stable, and wash-free imaging capabilities of <strong>O-698</strong> make it a promising probe for real-time monitoring of RNA behavior, providing valuable insights into drug mechanisms and therapeutic efficacy.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"107 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-02DOI: 10.1016/j.snb.2025.137709
Yani Liu, Haoyu Chen, Lin Chai, Jing Liu, Shu Huang, Feng Liu, Xiaohua Zhu, Youyu Zhang, Meiling Liu, Shouzhuo Yao
The emergence of various nanozymes has revolutionized approaches to detecting total antioxidant capacity (TAC) in food and biological samples; however, the precise quantification of specific antioxidant species such as ascorbic acid (AA) and glutathione (GSH) continue to present significant challenges. Inspired by the functional characteristics of oxidoreductases, we have developed Cu-CeO₂ nanoparticles adorned PCN-224, which exhibit multiple mimetic behaviors, including oxidase (OXD), ascorbate oxidase (AAO), and glutathione peroxidase (GPx)-like activities. This innovative PCN-224@Cu-CeO2 is designed for the quantitative evaluation of TAC in food, pharmaceuticals, and biological samples. Simultaneously, it enables the accurate determination of AA and GSH levels with favorable selectivity. The integration of nanozymes onto metal-organic frameworks significantly enhances both stability and catalytic efficiency. Furthermore, utilizing the multi-mimetics activity of the same materials simplifies the biosensing processes for TAC and individual antioxidants in tablets, beverages, fruits, and cells, thereby considerably diminishing experimental intricacy. Our findings not only advance the development and applications of novel nanozymes with exceptional multi-mimetic properties but also tackle selectivity challenges associated with colorimetric detection platforms that rely on oxidase and peroxidase activities.
{"title":"Total antioxidant capacity assessment and accurate quantification of ascorbic acid and glutathione utilizing the enhanced multi-mimetic of Cu-CeO₂ NPs decorated PCN-224","authors":"Yani Liu, Haoyu Chen, Lin Chai, Jing Liu, Shu Huang, Feng Liu, Xiaohua Zhu, Youyu Zhang, Meiling Liu, Shouzhuo Yao","doi":"10.1016/j.snb.2025.137709","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137709","url":null,"abstract":"The emergence of various nanozymes has revolutionized approaches to detecting total antioxidant capacity (TAC) in food and biological samples; however, the precise quantification of specific antioxidant species such as ascorbic acid (AA) and glutathione (GSH) continue to present significant challenges. Inspired by the functional characteristics of oxidoreductases, we have developed Cu-CeO₂ nanoparticles adorned PCN-224, which exhibit multiple mimetic behaviors, including oxidase (OXD), ascorbate oxidase (AAO), and glutathione peroxidase (GPx)-like activities. This innovative PCN-224@Cu-CeO<sub>2</sub> is designed for the quantitative evaluation of TAC in food, pharmaceuticals, and biological samples. Simultaneously, it enables the accurate determination of AA and GSH levels with favorable selectivity. The integration of nanozymes onto metal-organic frameworks significantly enhances both stability and catalytic efficiency. Furthermore, utilizing the multi-mimetics activity of the same materials simplifies the biosensing processes for TAC and individual antioxidants in tablets, beverages, fruits, and cells, thereby considerably diminishing experimental intricacy. Our findings not only advance the development and applications of novel nanozymes with exceptional multi-mimetic properties but also tackle selectivity challenges associated with colorimetric detection platforms that rely on oxidase and peroxidase activities.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"32 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-02DOI: 10.1016/j.snb.2025.137742
Ping Li, Ying Yang, Feng Li, Wenyuan Pei, Dan Li, Hui Yu, Xiangting Dong, Tianqi Wang
With the significant increase in global awareness of environmental protection, green non-toxic gas sensors have become an important tool to promote sustainable development. In this work, a gas sensor based on Bi2S3 green non-toxic semiconductor material was successfully prepared. By introducing polyoxometalate (POMs) electron acceptor into Bi2S3 sensing material, its detection ability for NO2 gas was significantly improved. At room temperature (25°C), the Bi2S3/1%PW12 sensor has a response value of 41.88 to 100 ppm NO2, which is 4.36 times the response value of the Bi2S3 sensor (9.61), highlighting its excellent detection performance. In addition, we thoroughly evaluated the selectivity, repeatability and other key gas sensing parameters of the sensor, further confirming its high-potential in practical applications. Through analysis of the mechanism underlying the performance enhancement, it is found that the appropriate addition of PW12 as an electron acceptor can effectively inhibit the electron-hole recombination, thus significantly improving the performance of the sensor. This important discovery opens up a new way and method for the performance optimization of gas sensors, and is expected to inject new vitality into the future development of gas sensor technology.
{"title":"Rapid room-temperature NO2 detection based on Bi2S3/polyoxometalate heterostructures: the synergy of increased electron transportation and heterojunction effect","authors":"Ping Li, Ying Yang, Feng Li, Wenyuan Pei, Dan Li, Hui Yu, Xiangting Dong, Tianqi Wang","doi":"10.1016/j.snb.2025.137742","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137742","url":null,"abstract":"With the significant increase in global awareness of environmental protection, green non-toxic gas sensors have become an important tool to promote sustainable development. In this work, a gas sensor based on Bi<sub>2</sub>S<sub>3</sub> green non-toxic semiconductor material was successfully prepared. By introducing polyoxometalate (POMs) electron acceptor into Bi<sub>2</sub>S<sub>3</sub> sensing material, its detection ability for NO<sub>2</sub> gas was significantly improved. At room temperature (25°C), the Bi<sub>2</sub>S<sub>3</sub>/1%PW<sub>12</sub> sensor has a response value of 41.88 to 100 ppm NO<sub>2</sub>, which is 4.36 times the response value of the Bi<sub>2</sub>S<sub>3</sub> sensor (9.61), highlighting its excellent detection performance. In addition, we thoroughly evaluated the selectivity, repeatability and other key gas sensing parameters of the sensor, further confirming its high-potential in practical applications. Through analysis of the mechanism underlying the performance enhancement, it is found that the appropriate addition of PW<sub>12</sub> as an electron acceptor can effectively inhibit the electron-hole recombination, thus significantly improving the performance of the sensor. This important discovery opens up a new way and method for the performance optimization of gas sensors, and is expected to inject new vitality into the future development of gas sensor technology.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"20 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-01DOI: 10.1016/j.snb.2025.137713
Hanxu Ma, Xiaorong Sun, Shunda Qiao, Ying He, Chu Zhang, Yufei Ma
This paper reports for the first time a high-performance light-induced thermoelastic spectroscopy (LITES) sensor based on a high-quality factor (Q) quartz tuning fork (QTF) as the load. The high-Q QTF, sealed in a vacuum and featuring a Q factor of about 50000, is connected in series with a commercially available standard QTF to enable piezoelectric signal filtering, thereby increasing the overall Q-factor. Methane (CH4), a highly flammable and explosive gas, is chosen as the target for this study. Compared to the bare QTF mode, the series QTF mode increased the Q value of the QTF detector by 2.11 times and the signal-to-noise ratio (SNR) of the CH4-LITES sensor by 1.73 times. Under varying CH4 concentrations, the series QTF mode-based CH4-LITES sensor demonstrated an excellent linear response, with a calculated minimum detection limit (MDL) of 0.96 ppm. According to Allan deviation analysis, the MDL of the CH4-LITES sensor was improved to 0.16 ppm when the average time of the system was 100 s. Additionally, to assess the frequency selectivity of the series QTF mode, external acoustic waves with a center frequency of 32.768 kHz were used for interference testing. The SNR of the bare QTF mode decreased by a factor of 108.92, while the SNR of the series QTF mode only decreased by a factor of 26.88. These results show that the series QTF mode provides 4 times greater anti-noise capacity, significantly enhancing the frequency selectivity of the LITES sensor.
{"title":"A high-performance light-induced thermoelastic spectroscopy sensor based on a high-Q value quartz tuning fork load","authors":"Hanxu Ma, Xiaorong Sun, Shunda Qiao, Ying He, Chu Zhang, Yufei Ma","doi":"10.1016/j.snb.2025.137713","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137713","url":null,"abstract":"This paper reports for the first time a high-performance light-induced thermoelastic spectroscopy (LITES) sensor based on a high-quality factor (<em>Q</em>) quartz tuning fork (QTF) as the load. The high-Q QTF, sealed in a vacuum and featuring a <em>Q</em> factor of about 50000, is connected in series with a commercially available standard QTF to enable piezoelectric signal filtering, thereby increasing the overall Q-factor. Methane (CH<sub>4</sub>), a highly flammable and explosive gas, is chosen as the target for this study. Compared to the bare QTF mode, the series QTF mode increased the <em>Q</em> value of the QTF detector by 2.11 times and the signal-to-noise ratio (SNR) of the CH<sub>4</sub>-LITES sensor by 1.73 times. Under varying CH<sub>4</sub> concentrations, the series QTF mode-based CH<sub>4</sub>-LITES sensor demonstrated an excellent linear response, with a calculated minimum detection limit (MDL) of 0.96 ppm. According to Allan deviation analysis, the MDL of the CH<sub>4</sub>-LITES sensor was improved to 0.16 ppm when the average time of the system was 100<!-- --> <!-- -->s. Additionally, to assess the frequency selectivity of the series QTF mode, external acoustic waves with a center frequency of 32.768<!-- --> <!-- -->kHz were used for interference testing. The SNR of the bare QTF mode decreased by a factor of 108.92, while the SNR of the series QTF mode only decreased by a factor of 26.88. These results show that the series QTF mode provides 4 times greater anti-noise capacity, significantly enhancing the frequency selectivity of the LITES sensor.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"75 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-01DOI: 10.1016/j.snb.2025.137738
Chong Tan , Yu Cao , Nan Xie , Mingxiang Zhang , Lili Liu , Haichao Yu , Changhong Wang , Yaowen Jiang , Yuanming Wu , Zhen Yuan , Zaihua Duan , Yadong Jiang , Huiling Tai
The traditional respiratory rate detection techniques, such as clinic monitor and ventilator, are often limited to specific application scenarios due to their complexity and high cost. In this work, we propose a disposable and cost-effective paper-based (PB) LiCl humidity sensor. The results show that the PB LiCl humidity sensor can detect a relative humidity (RH) range of 28.8–91.5 %, with alternating current impedance of 562.9 and direct current resistance response of 10,928.8 at 91.5 % RH (25°C). Notably, the incorporation of LiCl reduces the resistance of the paper material, which makes it easier for the circuit to obtain the resistance value. To achieve automatic recognition of respiratory rate, we propose a precise peak-seeking algorithm based on extreme value detection, addressing the baseline drift issue encountered in actual respiratory detection by humidity sensor. The corresponding circuit system is designed, which can accurately capture and analyze humidity changes caused by respiration, generate real-time respiratory response curves, and calculate respiratory rates, achieving intelligent respiratory rate detection. This work provides a useful reference for developing portable and low-cost respiratory detection equipment based on the disposable PB LiCl humidity sensor.
{"title":"Intelligent respiratory rate detection using disposable paper-based humidity sensor and precise peak-seeking algorithm","authors":"Chong Tan , Yu Cao , Nan Xie , Mingxiang Zhang , Lili Liu , Haichao Yu , Changhong Wang , Yaowen Jiang , Yuanming Wu , Zhen Yuan , Zaihua Duan , Yadong Jiang , Huiling Tai","doi":"10.1016/j.snb.2025.137738","DOIUrl":"10.1016/j.snb.2025.137738","url":null,"abstract":"<div><div>The traditional respiratory rate detection techniques, such as clinic monitor and ventilator, are often limited to specific application scenarios due to their complexity and high cost. In this work, we propose a disposable and cost-effective paper-based (PB) LiCl humidity sensor. The results show that the PB LiCl humidity sensor can detect a relative humidity (RH) range of 28.8–91.5 %, with alternating current impedance of 562.9 and direct current resistance response of 10,928.8 at 91.5 % RH (25°C). Notably, the incorporation of LiCl reduces the resistance of the paper material, which makes it easier for the circuit to obtain the resistance value. To achieve automatic recognition of respiratory rate, we propose a precise peak-seeking algorithm based on extreme value detection, addressing the baseline drift issue encountered in actual respiratory detection by humidity sensor. The corresponding circuit system is designed, which can accurately capture and analyze humidity changes caused by respiration, generate real-time respiratory response curves, and calculate respiratory rates, achieving intelligent respiratory rate detection. This work provides a useful reference for developing portable and low-cost respiratory detection equipment based on the disposable PB LiCl humidity sensor.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"436 ","pages":"Article 137738"},"PeriodicalIF":8.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Antibiotic resistance genes (ARGs), especially extracellular ARGs (eARGs), are recognized as emerging pollutants that pose significant threats to public health and the environment, characterized by their increased transmission risk across bacterial genera and the difficulty in monitoring them. Herein, a comprehensive method for the efficient detection of eARGs was developed, integrating magnetic solid phase extraction (MSPE), light-driven (LD) recombinase polymerase amplification (RPA), and lateral flow assay (LFA). Initially, Fe3O4@polydopamine (Fe3O4@PDA) was utilized as the adsorbent in MSPE to extract eARGs from samples. The extracellular sulfonamide resistance gene sul1 was chosen as a model analyte for this study. Subsequently, the efficiency of nucleic acid amplification was significantly enhanced by 2.2 times compared to traditional water-bathed RPA, through the implementation of LD-RPA. This technique employed a portable laser flashlight as the light source and Fe3O4@PDA as the photosensitive medium, facilitating rapid and efficient amplification of the target gene. Following amplification, the specifically designed LFA was utilized for the detection of the amplicons. The resulting assay demonstrated a wide linear range of 10 copies/µL to 1.0 × 105 copies/µL in the eluent of MSPE (R2 = 0.9939), with a remarkable detection limit of 10 copies/µL eluent, corresponding to 3.3 × 10-2 copies/µL in water samples. The entire analytical procedure costs only 35 min. To validate the method, environmental water samples were tested, and the results obtained were found to be in good agreement with those from real-time quantitative polymerase chain reaction, further substantiating the accuracy and efficacy of the integrated MSPE–LD-RPA–LFA approach.
{"title":"On-Site Detection of Extracellular Antibiotic Resistance Genes by Magnetic Solid Phase Extraction−Light Driven Amplification−Lateral Flow Assay","authors":"Yi-Nan Yang, Zhao-Hui Wang, Yue Huang, Li-xia Yan, Xiao-Dong Huang, Wen-Long Wang, Wen-Feng Zhao, Yong-Wei Feng, Yi Zhang","doi":"10.1016/j.snb.2025.137707","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137707","url":null,"abstract":"Antibiotic resistance genes (ARGs), especially extracellular ARGs (eARGs), are recognized as emerging pollutants that pose significant threats to public health and the environment, characterized by their increased transmission risk across bacterial genera and the difficulty in monitoring them. Herein, a comprehensive method for the efficient detection of eARGs was developed, integrating magnetic solid phase extraction (MSPE), light-driven (LD) recombinase polymerase amplification (RPA), and lateral flow assay (LFA). Initially, Fe<sub>3</sub>O<sub>4</sub>@polydopamine (Fe<sub>3</sub>O<sub>4</sub>@PDA) was utilized as the adsorbent in MSPE to extract eARGs from samples. The extracellular sulfonamide resistance gene <em>sul1</em> was chosen as a model analyte for this study. Subsequently, the efficiency of nucleic acid amplification was significantly enhanced by 2.2 times compared to traditional water-bathed RPA, through the implementation of LD-RPA. This technique employed a portable laser flashlight as the light source and Fe<sub>3</sub>O<sub>4</sub>@PDA as the photosensitive medium, facilitating rapid and efficient amplification of the target gene. Following amplification, the specifically designed LFA was utilized for the detection of the amplicons. The resulting assay demonstrated a wide linear range of 10 copies/µL to 1.0 × 10<sup>5</sup> copies/µL in the eluent of MSPE (R<sup>2</sup> = 0.9939), with a remarkable detection limit of 10 copies/µL eluent, corresponding to 3.3 × 10<sup>-2</sup> copies/µL in water samples. The entire analytical procedure costs only 35<!-- --> <!-- -->min. To validate the method, environmental water samples were tested, and the results obtained were found to be in good agreement with those from real-time quantitative polymerase chain reaction, further substantiating the accuracy and efficacy of the integrated MSPE–LD-RPA–LFA approach.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"14 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-01DOI: 10.1016/j.snb.2025.137712
Sarah Hernandez, Dhruv Miglani, Jayden Kimbro, Alessandra Luna, Heather Jones, Benjamin A. Pinsky, Jesse J. Waggoner, David R. Myers
Molecular tests offer a powerful approach to diagnosing numerous diseases and, due to advances in lab-on-a-chip technologies, have the potential to be used in the field and at the bedside. However, there is still a need for portable technologies that help isolate nucleic acids from their associated sample for testing. Previously, our groups developed an RNA extraction and stabilization (RNAES) protocol that utilized novel shelf stable reagents to extract, isolate, and store nucleic acids on membranes at ambient temperature. Building on these results, we have created RNAES kits with reductionist, 3D-printed, lab-on-a-chip style parts that enable field isolation of RNA without external equipment or power. Our kits feature three components, a lysis sphere, a capillary-wicking based cartridge for RNA isolation, as well as a drying & storage tube that stabilizes RNA for ambient temperature shipping. Using our kit, a user can process a 25 µL sample of serum or plasma within 15 minutes, including a 10-minute incubation time. Noting that hepatitis C virus (HCV) can be cured when detected, we use our RNAES kit in combination with a new real-time RT-PCR to detect HCV. Our data on 98 clinical samples and four contrived whole blood samples show comparable RNA detection and quantified viral loads following extraction with our RNAES kit or a commercial system. Our approach also provided the additional benefit of stabilizing viral RNA for storage at ambient temperatures as lysate, for up to 7 days, or on dried membranes in storage tubes, for at least 30 days.
{"title":"Portable hepatitis C virus RNA extraction and stabilization using low-cost lab-on-a-chip style components with shelf stable reagents","authors":"Sarah Hernandez, Dhruv Miglani, Jayden Kimbro, Alessandra Luna, Heather Jones, Benjamin A. Pinsky, Jesse J. Waggoner, David R. Myers","doi":"10.1016/j.snb.2025.137712","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137712","url":null,"abstract":"Molecular tests offer a powerful approach to diagnosing numerous diseases and, due to advances in lab-on-a-chip technologies, have the potential to be used in the field and at the bedside. However, there is still a need for portable technologies that help isolate nucleic acids from their associated sample for testing. Previously, our groups developed an <u>RNA e</u>xtraction and <u>s</u>tabilization (RNAES) protocol that utilized novel shelf stable reagents to extract, isolate, and store nucleic acids on membranes at ambient temperature. Building on these results, we have created RNAES kits with reductionist, 3D-printed, lab-on-a-chip style parts that enable field isolation of RNA without external equipment or power. Our kits feature three components, a lysis sphere, a capillary-wicking based cartridge for RNA isolation, as well as a drying & storage tube that stabilizes RNA for ambient temperature shipping. Using our kit, a user can process a 25<!-- --> <!-- -->µL sample of serum or plasma within 15<!-- --> <!-- -->minutes, including a 10-minute incubation time. Noting that hepatitis C virus (HCV) can be cured when detected, we use our RNAES kit in combination with a new real-time RT-PCR to detect HCV. Our data on 98 clinical samples and four contrived whole blood samples show comparable RNA detection and quantified viral loads following extraction with our RNAES kit or a commercial system. Our approach also provided the additional benefit of stabilizing viral RNA for storage at ambient temperatures as lysate, for up to 7 days, or on dried membranes in storage tubes, for at least 30 days.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"183 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-01DOI: 10.1016/j.snb.2025.137737
Joowon Seo, Junsuk Kim, Beomjoon Kim, Ali Mani, Sungjae Ha, Sung Jae Kim
Accurate in operando measurement of electric potential in electrokinetic systems is critical yet challenging due to complications arising from electrochemical reactions and electrical double layer capacitance at the metal electrode-electrolyte interface. These challenges are further compounded under in operando conditions requiring simultaneous voltage application and measurement, where conventional probes often induce leakage currents and distort measurements. To overcome these limitations, we developed an ion-selective membrane (ISM) probe with high input impedance, capable of transferring charge as a form of ion, thereby suppressing redox reactions and eliminating leakage currents. Integrated into a microfluidic platform, the ISM probe demonstrated robust performance across a wide resistance range, with reliable operation even in giga-ohm environments, a suitable cutoff frequency for tracking dynamic potentials, and significantly reduced noise and measurement offset compared to traditional metal-based probes. Leveraging these advantages, we employed the ISM probe to perform in operando dynamic analysis of ion concentration, particularly focusing on electrokinetic phenomena such as ion concentration polarization. The probe enabled precise spatial and temporal measurement of concentration gradients formed by ion concentration polarization, offering new insights into ion distribution dynamics; (1) issues in visualizing ion concentrations using fluorescence and (2) the second plateau of ion concentration near nanoporous membranes. This work establishes the ISM probe as a versatile tool for advancing the understanding of ion profiles in complex electrokinetic systems, addressing key challenges in both fundamental research and practical applications.
{"title":"in operando Spatiotemporal Analysis of Ion Concentration Profile Using Ion-Selective Membrane Probes in Electrokinetic Systems","authors":"Joowon Seo, Junsuk Kim, Beomjoon Kim, Ali Mani, Sungjae Ha, Sung Jae Kim","doi":"10.1016/j.snb.2025.137737","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137737","url":null,"abstract":"Accurate <em>in operando</em> measurement of electric potential in electrokinetic systems is critical yet challenging due to complications arising from electrochemical reactions and electrical double layer capacitance at the metal electrode-electrolyte interface. These challenges are further compounded under <em>in operando</em> conditions requiring simultaneous voltage application and measurement, where conventional probes often induce leakage currents and distort measurements. To overcome these limitations, we developed an ion-selective membrane (ISM) probe with high input impedance, capable of transferring charge as a form of ion, thereby suppressing redox reactions and eliminating leakage currents. Integrated into a microfluidic platform, the ISM probe demonstrated robust performance across a wide resistance range, with reliable operation even in giga-ohm environments, a suitable cutoff frequency for tracking dynamic potentials, and significantly reduced noise and measurement offset compared to traditional metal-based probes. Leveraging these advantages, we employed the ISM probe to perform <em>in operando</em> dynamic analysis of ion concentration, particularly focusing on electrokinetic phenomena such as ion concentration polarization. The probe enabled precise spatial and temporal measurement of concentration gradients formed by ion concentration polarization, offering new insights into ion distribution dynamics; (1) issues in visualizing ion concentrations using fluorescence and (2) the second plateau of ion concentration near nanoporous membranes. This work establishes the ISM probe as a versatile tool for advancing the understanding of ion profiles in complex electrokinetic systems, addressing key challenges in both fundamental research and practical applications.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"38 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-01DOI: 10.1016/j.snb.2025.137722
Sachin Ganpat Chavan, Pooja Ramrao Rathod, Aneesh Koyappayil, Gopi Karuppaiah, Anna Go, Eunjian Jin, Jihoon Park, Min-Ho Lee
Individualized tracking of female hormones progesterone level raises a significant impact on women's health, including fertility and carcinogenic risks. Nevertheless, current methods are difficult to use indoors because they typically call for intrusive sampling or large analytical laboratory equipment. Here, we report a straightforward electrochemical biosensor based on the aptamer-target-induced displacement of strands for the automated and minimally invasive diagnostic progesterone via in situ serum and sweat monitoring. This detection screen-printed carbon electrode (SPCE) combines a reagent-less signal enhancement and 'signal-on' recognition strategy with a gold nanoparticle (AuNPs) decorated via (3-aminopropyl) triethoxysilane (APTES) functionalized Nb2C (niobium carbide) MXene nanosheets achieves high specificity and exceptional sensitivity at picomolar concentrations. The device could detect progesterone levels linearly within the 50 to 160 pM range, with a sensitivity of 0.159 µA pM−1 and a limit of detection (LOD) of 14 pM in PBS buffer (pH 7.4) and 17 pM in the spiked female sweat sample. Our integrated aptamer-based SPCE system was successfully demonstrated in real-time clinical samples at point-of-care testing. Aptamer modification on AuNPs-APTES-Nb2C-MXene nanosheets opens up new possibilities for biomedical and electrochemical sensing applications in progesterone detection at picomolar concentration exhibits exceptional stability, repeatability, specificity, sensitivity, and selectivity.
{"title":"Self-assembled AuNPs on Niobium Carbide (Nb2C) MXene-based Apta-sensor for Progesterone Recognition in Female Sweat and Serum Sample","authors":"Sachin Ganpat Chavan, Pooja Ramrao Rathod, Aneesh Koyappayil, Gopi Karuppaiah, Anna Go, Eunjian Jin, Jihoon Park, Min-Ho Lee","doi":"10.1016/j.snb.2025.137722","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137722","url":null,"abstract":"Individualized tracking of female hormones progesterone level raises a significant impact on women's health, including fertility and carcinogenic risks. Nevertheless, current methods are difficult to use indoors because they typically call for intrusive sampling or large analytical laboratory equipment. Here, we report a straightforward electrochemical biosensor based on the aptamer-target-induced displacement of strands for the automated and minimally invasive diagnostic progesterone via in situ serum and sweat monitoring. This detection screen-printed carbon electrode (SPCE) combines a reagent-less signal enhancement and 'signal-on' recognition strategy with a gold nanoparticle (AuNPs) decorated via (3-aminopropyl) triethoxysilane (APTES) functionalized Nb<sub>2</sub>C (niobium carbide) MXene nanosheets achieves high specificity and exceptional sensitivity at picomolar concentrations. The device could detect progesterone levels linearly within the 50 to 160 pM range, with a sensitivity of 0.159<!-- --> <!-- -->µA pM<sup>−1</sup> and a limit of detection (LOD) of 14 pM in PBS buffer (pH 7.4) and 17 pM in the spiked female sweat sample. Our integrated aptamer-based SPCE system was successfully demonstrated in real-time clinical samples at point-of-care testing. Aptamer modification on AuNPs-APTES-Nb<sub>2</sub>C-MXene nanosheets opens up new possibilities for biomedical and electrochemical sensing applications in progesterone detection at picomolar concentration exhibits exceptional stability, repeatability, specificity, sensitivity, and selectivity.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"107 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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