With the growing global emphasis on environmental protection, new energy vehicles have become essential for reducing carbon emissions in the transportation sector. However, safety issues related to lithium-ion batteries, particularly thermal runaway, remain a critical concern. Different stages of thermal runaway produce distinct gas compositions, necessitating sensors with high selectivity for targeted detection of specific gases or gas categories. Dynamic measurement technology using temperature modulation can enhance the selectivity of semiconductor gas sensors. However, most dynamic measurements yield limited data features for gas categories, complicating subsequent classification algorithms and making them less suitable for deployment in embedded devices. To address these challenges, this study proposes an electronic nose system based on hybrid waveform modulation technology. By employing multi-waveform superposition heating, this approach enriches data features corresponding to gas responses and optimizes sensor technology and data processing algorithms using ARM+FPGA architectures, significantly improving system accuracy. The system collects gas sensor data via a sensor array and achieves a recognition rate of 95.82% using the MLP algorithm, successfully deployed on Xilinx’s System-on-Chip (SoC) platform.
{"title":"Gas Classification System Based on Hybrid Waveform Modulation Technology on FPGA","authors":"Jiade Zhang, Mingzhi Jiao, Liangsong Duan, Lina Zheng, VanDuy Nguyen, Chu Manh Hung, DucHoa Nguyen","doi":"10.1016/j.snb.2025.137637","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137637","url":null,"abstract":"With the growing global emphasis on environmental protection, new energy vehicles have become essential for reducing carbon emissions in the transportation sector. However, safety issues related to lithium-ion batteries, particularly thermal runaway, remain a critical concern. Different stages of thermal runaway produce distinct gas compositions, necessitating sensors with high selectivity for targeted detection of specific gases or gas categories. Dynamic measurement technology using temperature modulation can enhance the selectivity of semiconductor gas sensors. However, most dynamic measurements yield limited data features for gas categories, complicating subsequent classification algorithms and making them less suitable for deployment in embedded devices. To address these challenges, this study proposes an electronic nose system based on hybrid waveform modulation technology. By employing multi-waveform superposition heating, this approach enriches data features corresponding to gas responses and optimizes sensor technology and data processing algorithms using ARM+FPGA architectures, significantly improving system accuracy. The system collects gas sensor data via a sensor array and achieves a recognition rate of 95.82% using the MLP algorithm, successfully deployed on Xilinx’s System-on-Chip (SoC) platform.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"90 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-20DOI: 10.1016/j.snb.2025.137640
Filippo Vurro, Elena Dembech, Riccardo Manfredi, Gabriele Debbi, Manuele Bettelli, Alice Marinangeli, Alessandra Maria Bossi, Nadia Palermo, Vittoria Martini, Michela Janni, Nicola Coppedè
Organic electrochemical transistors (OECTs) have been used as flexible biosensors, in organic bioelectronics, with high sensitivity and high transconductance but limited selectivity. OECTs can measure metabolic biomarkers, also continuously with real-time monitoring applications, in different biofluids of interest, with applications in sports, healthcare, biology and agriculture.In this study we developed an OECTs biosensor based on the functionalization of the active channel of the OECT with a biomimetic recognition element, namely molecularly imprinted nanoparticles (nanoMIPs), to selectively bind the target analyte D-glucose. Two configurations based on textile absorbent materials (nanoMIP fiber wire) or on polymer microfibers (nanoMIP microwire) were prepared and tested for D-glucose sensing and for D-fructose interference. The results show that the nanoMIPs improved the sensitivity and selectivity towards D-glucose. The nanoMIP fiber D-glucose wire sensor was used to monitor tomato plants in the field together with the conventional OECT based biosensor bioristor, providing new insights into the dynamics of the drought defense response.
{"title":"Glucose selective textile OECT based on Molecularly Imprinted Nanoparticles functionalized channel for in vivo plants monitoring","authors":"Filippo Vurro, Elena Dembech, Riccardo Manfredi, Gabriele Debbi, Manuele Bettelli, Alice Marinangeli, Alessandra Maria Bossi, Nadia Palermo, Vittoria Martini, Michela Janni, Nicola Coppedè","doi":"10.1016/j.snb.2025.137640","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137640","url":null,"abstract":"Organic electrochemical transistors (OECTs) have been used as flexible biosensors, in organic bioelectronics, with high sensitivity and high transconductance but limited selectivity. OECTs can measure metabolic biomarkers, also continuously with real-time monitoring applications, in different biofluids of interest, with applications in sports, healthcare, biology and agriculture.In this study we developed an OECTs biosensor based on the functionalization of the active channel of the OECT with a biomimetic recognition element, namely molecularly imprinted nanoparticles (nanoMIPs), to selectively bind the target analyte D-glucose. Two configurations based on textile absorbent materials (nanoMIP fiber wire) or on polymer microfibers (nanoMIP microwire) were prepared and tested for D-glucose sensing and for D-fructose interference. The results show that the nanoMIPs improved the sensitivity and selectivity towards D-glucose. The nanoMIP fiber D-glucose wire sensor was used to monitor tomato plants in the field together with the conventional OECT based biosensor bioristor, providing new insights into the dynamics of the drought defense response.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"34 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-20DOI: 10.1016/j.snb.2025.137648
Ying Liu, Siyuan Wang, Xinyi Qu, Yuanfang Li, Cuiping Zhou, Dong Xie, Jianpei Dong, Li Ji, Jincheng Xu, Jianhua Zhou
Accurate detection and early diagnosis of periodontitis can be potentially achieved by detecting volatile sulfur compounds (VSCs) produced by pathogenic bacteria beneath the gingiva. However, current methods for detecting VSCs face limitations due to their reliance on expensive and bulky instruments, long testing times, or the requirement of customized mouthguards. Herein, we propose a simple yet effective strategy to improve the sensitivity of ZnO quantum dots (QDs) to VSCs by modulating surface defects of ZnO QDs and report the development of the as-prepared highly sensitive fluorescent ZnO-PDMS wearable dental patch sensor. With the assistance of artificial intelligence (AI) recognizing the fluorescence quenching areas, the ZnO-PDMS dental patches are demonstrated to accurately detect the local release of VSCs (e.g., H2S) in vivo within 15 min, thereby enabling convenient mapping and screening for hidden periodontitis lesions. Due to its facile preparation, ease of use, and rapid and accurate mapping ability, the ZnO-PDMS dental patches demonstrate significant potential in routine screening, treatment assisting, and the monitoring of periodontitis.
{"title":"Highly-sensitive ultra-thin dental patches assisted with artificial-intelligence recognition for mapping hidden periodontitis lesions","authors":"Ying Liu, Siyuan Wang, Xinyi Qu, Yuanfang Li, Cuiping Zhou, Dong Xie, Jianpei Dong, Li Ji, Jincheng Xu, Jianhua Zhou","doi":"10.1016/j.snb.2025.137648","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137648","url":null,"abstract":"Accurate detection and early diagnosis of periodontitis can be potentially achieved by detecting volatile sulfur compounds (VSCs) produced by pathogenic bacteria beneath the gingiva. However, current methods for detecting VSCs face limitations due to their reliance on expensive and bulky instruments, long testing times, or the requirement of customized mouthguards. Herein, we propose a simple yet effective strategy to improve the sensitivity of ZnO quantum dots (QDs) to VSCs by modulating surface defects of ZnO QDs and report the development of the as-prepared highly sensitive fluorescent ZnO-PDMS wearable dental patch sensor. With the assistance of artificial intelligence (AI) recognizing the fluorescence quenching areas, the ZnO-PDMS dental patches are demonstrated to accurately detect the local release of VSCs (e.g., H<sub>2</sub>S) <em>in vivo</em> within 15<!-- --> <!-- -->min, thereby enabling convenient mapping and screening for hidden periodontitis lesions. Due to its facile preparation, ease of use, and rapid and accurate mapping ability, the ZnO-PDMS dental patches demonstrate significant potential in routine screening, treatment assisting, and the monitoring of periodontitis.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"37 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aggregation-induced emission luminogens (AIEgens)-loaded nanoparticles (NPs) with unique optical properties have recently emerged as valuable tools in lateral flow immunoassays (LFIA). To further enhance the sensitivity and reliability of LFIA, there is an ongoing demand for fluorescent NPs exhibiting higher brightness. Herein, we developed an ultrabright AIEgens NPs-based LFIA for rapid and quantitative detection of the acute myocardial infarction biomarker, cardiac troponin I (cTnI). We introduced two para-CF3-substituted benzenes (p-CF3-Ph) groups into the traditional AIE structure to form (E)-4-(4-(bis(4'-(trifluoromethyl)-[1,1'-biphenyl]-4-yl)amino)styryl)-1-octadecylpyridin-1-ium (CF3-TPA). The lipophilicity-improved CF3-TPA was encapsulated into the hydrophobic cavities of polystyrene microspheres (PS), resulting in AIECF3NPs. Gratifyingly, the quantum yield (QY) of AIECF3NPs conjugated with antibodies (AIECF3NPs-mAb1) remained at 47.24%, basically consistent with 48.68% of AIECF3NPs, which guarantees the sensitivity of the strip. Additionally, the Stokes shift of AIECF3NPs reached 125 nm, effectively minimizing significant background fluorescence in the visible region. AIECF3NPs-labeled LFIA showed high sensitivity for cTnI detection in the range of 1-100 ng/ml, with a limit of detection (LOD) of 0.0897 ng/mL and a visible LOD of 10 ng/mL within 10 minutes, demonstrating high specificity and accuracy. Overall, AIECF3NPs has great potential as an effective tool for disease diagnosis in emergency situations.
{"title":"Reasonable Design of Aggregation-Induced Emission Luminogens for Rapid Detection of Cardiac Troponin I by Lateral Flow Immunoassay","authors":"Panpan Sun, Qian Li, Ningshuang Gao, Mingyue Luo, Wenzhuo Chang, Baodui Wang, Xiaoquan Lu, Zhonghua Xue","doi":"10.1016/j.snb.2025.137635","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137635","url":null,"abstract":"Aggregation-induced emission luminogens (AIEgens)-loaded nanoparticles (NPs) with unique optical properties have recently emerged as valuable tools in lateral flow immunoassays (LFIA). To further enhance the sensitivity and reliability of LFIA, there is an ongoing demand for fluorescent NPs exhibiting higher brightness. Herein, we developed an ultrabright AIEgens NPs-based LFIA for rapid and quantitative detection of the acute myocardial infarction biomarker, cardiac troponin I (cTnI). We introduced two <em>para</em>-CF<sub>3</sub>-substituted benzenes (<em>p</em>-CF<sub>3</sub>-Ph) groups into the traditional AIE structure to form (E)-4-(4-(bis(4'-(trifluoromethyl)-[1,1'-biphenyl]-4-yl)amino)styryl)-1-octadecylpyridin-1-ium (CF<sub>3</sub>-TPA). The lipophilicity-improved CF<sub>3</sub>-TPA was encapsulated into the hydrophobic cavities of polystyrene microspheres (PS), resulting in AIE<sub>CF3</sub>NPs. Gratifyingly, the quantum yield (QY) of AIE<sub>CF3</sub>NPs conjugated with antibodies (AIE<sub>CF3</sub>NPs-mAb1) remained at 47.24%, basically consistent with 48.68% of AIE<sub>CF3</sub>NPs, which guarantees the sensitivity of the strip. Additionally, the Stokes shift of AIE<sub>CF3</sub>NPs reached 125<!-- --> <!-- -->nm, effectively minimizing significant background fluorescence in the visible region. AIE<sub>CF3</sub>NPs-labeled LFIA showed high sensitivity for cTnI detection in the range of 1-100<!-- --> <!-- -->ng/ml, with a limit of detection (LOD) of 0.0897<!-- --> <!-- -->ng/mL and a visible LOD of 10<!-- --> <!-- -->ng/mL within 10<!-- --> <!-- -->minutes, demonstrating high specificity and accuracy. Overall, AIE<sub>CF3</sub>NPs has great potential as an effective tool for disease diagnosis in emergency situations.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"20 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-19DOI: 10.1016/j.snb.2025.137645
Ying-Ying Kong, Bo Liu, Yu-Hang Zhang, Di Han, Hong-Kun Yang, Jun-Tang Lin, Hong-Cheng Xia
Acute kidney injury (AKI) has attracted attention as a frequent clinical disease, and the occurrence of AKI can lead to the decline of kidney function and even endanger life. Realizing early diagnosis of AKI can effectively control and treat the condition. Current clinical diagnostic methods cannot meet the accurate diagnosis of early acute kidney injury. Oxidative stress has been proved to be a causative factor of AKI. Therefore, we constructed a fluorescent probe based on coumarin fluorophore for the detection of hypochlorite (HClO) produced during oxidative stress to realize early diagnosis of AKI. The structure of the probe is simple and easy to synthesize, and it has the ability of colorimetric and fluorescence recognition for HClO. The probe responds to HClO as fast as 3 seconds and shows good selectivity and sensitivity to HClO with a detection limit of 15.6 nM. The probe is also well used for imaging exogenous and endogenous HClO in cells. It also can be successfully used for imaging and diagnosis of cisplatin-induced acute kidney injury.
{"title":"Coumarin-based fluorescent probe for hypochlorite detection and imaging of acute kidney injury","authors":"Ying-Ying Kong, Bo Liu, Yu-Hang Zhang, Di Han, Hong-Kun Yang, Jun-Tang Lin, Hong-Cheng Xia","doi":"10.1016/j.snb.2025.137645","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137645","url":null,"abstract":"Acute kidney injury (AKI) has attracted attention as a frequent clinical disease, and the occurrence of AKI can lead to the decline of kidney function and even endanger life. Realizing early diagnosis of AKI can effectively control and treat the condition. Current clinical diagnostic methods cannot meet the accurate diagnosis of early acute kidney injury. Oxidative stress has been proved to be a causative factor of AKI. Therefore, we constructed a fluorescent probe based on coumarin fluorophore for the detection of hypochlorite (HClO) produced during oxidative stress to realize early diagnosis of AKI. The structure of the probe is simple and easy to synthesize, and it has the ability of colorimetric and fluorescence recognition for HClO. The probe responds to HClO as fast as 3<!-- --> <!-- -->seconds and shows good selectivity and sensitivity to HClO with a detection limit of 15.6<!-- --> <!-- -->nM. The probe is also well used for imaging exogenous and endogenous HClO in cells. It also can be successfully used for imaging and diagnosis of cisplatin-induced acute kidney injury.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"56 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-19DOI: 10.1016/j.snb.2025.137638
Shixin Huang, Wei Liu, Hailong Lin, Zhicheng Wen, Chunjin Hang, Rong An, Yongfeng Li, Yanhong Tian
The design of materials for the detection of n-butanol at low operating temperatures is of great significance for the reduction of power consumption and the improvement of the safety of the gas sensor. In this study, the solvothermal method and subsequent Ag-loading treatment were employed to synthesise hierarchical flower-like Cu-doped SnO2/Ag2S heterojunctions decorated with Ag (CSAA). The morphological and structural characterisation demonstrated that the amount of Cu2+ doping and the duration of electroless Ag plating played a pivotal role in the morphological evolution of the SnO2-based hierarchical structure. The optimal CSAA composite exhibits the highest response of 1136 and the shortest response time of 6 s to 50 ppm n-butanol at 80°C, accompanied by superior selectivity, repeatability, humidity and long-term stability. The enhanced sensing performances of the CSAA sensor can be mainly ascribed to the hierarchical porous structure, the doping of Cu2+, the construction of SnO2/Ag2S heterostructures and the decoration of Ag nanoparticles (Ag NPs). Furthermore, the density functional theory simulation was employed to investigate the influence of Ag NPs and Ag2S on the adsorption properties and electronic behaviour of n-butanol on the surface of SnO2. This work proposes a friendly strategy and theoretical support for enhancing the sensing performance of MOS sensors in the practical detection of n-butanol.
{"title":"Hierarchical flower-like Cu-doped SnO2/Ag2S heterojunctions decorated with Ag for excellent sensing performance toward n-butanol at low operating temperatures","authors":"Shixin Huang, Wei Liu, Hailong Lin, Zhicheng Wen, Chunjin Hang, Rong An, Yongfeng Li, Yanhong Tian","doi":"10.1016/j.snb.2025.137638","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137638","url":null,"abstract":"The design of materials for the detection of n-butanol at low operating temperatures is of great significance for the reduction of power consumption and the improvement of the safety of the gas sensor. In this study, the solvothermal method and subsequent Ag-loading treatment were employed to synthesise hierarchical flower-like Cu-doped SnO<sub>2</sub>/Ag<sub>2</sub>S heterojunctions decorated with Ag (CSAA). The morphological and structural characterisation demonstrated that the amount of Cu<sup>2+</sup> doping and the duration of electroless Ag plating played a pivotal role in the morphological evolution of the SnO<sub>2</sub>-based hierarchical structure. The optimal CSAA composite exhibits the highest response of 1136 and the shortest response time of 6 s to 50 ppm n-butanol at 80°C, accompanied by superior selectivity, repeatability, humidity and long-term stability. The enhanced sensing performances of the CSAA sensor can be mainly ascribed to the hierarchical porous structure, the doping of Cu<sup>2+</sup>, the construction of SnO<sub>2</sub>/Ag<sub>2</sub>S heterostructures and the decoration of Ag nanoparticles (Ag NPs). Furthermore, the density functional theory simulation was employed to investigate the influence of Ag NPs and Ag<sub>2</sub>S on the adsorption properties and electronic behaviour of n-butanol on the surface of SnO<sub>2</sub>. This work proposes a friendly strategy and theoretical support for enhancing the sensing performance of MOS sensors in the practical detection of n-butanol.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"15 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Group A rotavirus (RVA) is the main cause of acute gastroenteritis in infants and children with high infectivity, continuously presenting a negative impact on society. Therefore, the rapid and accurate detection of RVA is crucial for the prevention and diagnosis of the disease. Here we establish a modular and visual diagnostic platform for nucleic acid detection of the pathogen by integrating four technologies, asymmetric nucleic acid sequence-based amplification (asymmetric-NASBA), enzyme-free toehold-mediated strand displacement (TMSD), programmable primer exchange reaction (PER) cascades-mediated efficient signal amplification and a lateral flow immunoassay (LFIA), namely an ATPL-based platform. The built-in workflow can isothermally complete a convenient and rapid detection of the target molecules with a minimum concentration of 2.46 copies/mL in about 20 min, and markedly generate a lateral flow line signal for the naked eye. Moreover, a single-base difference can be distinguished from the target sequence, demonstrating high specificity and clinically analytical performance. Compared to the current common detection technology RT-qPCR, this platform provides detection results with high consistency with a kappa value of 0.927. In a word, the established ATPL-based detection platform is fast, highly sensitive and specific with promising accuracy, suggesting wide application potential in the detection of rotavirus or other nucleic acid in the future.
{"title":"An integrated platform for rotavirus nucleic acid detection","authors":"E-Bin Gao, Wenxi Lu, Yurun Hu, Qinggang Xu, Zhong Ni, Junhua Wu, Guoping Huang","doi":"10.1016/j.snb.2025.137642","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137642","url":null,"abstract":"Group A rotavirus (RVA) is the main cause of acute gastroenteritis in infants and children with high infectivity, continuously presenting a negative impact on society. Therefore, the rapid and accurate detection of RVA is crucial for the prevention and diagnosis of the disease. Here we establish a modular and visual diagnostic platform for nucleic acid detection of the pathogen by integrating four technologies, asymmetric nucleic acid sequence-based amplification (asymmetric-NASBA), enzyme-free toehold-mediated strand displacement (TMSD), programmable primer exchange reaction (PER) cascades-mediated efficient signal amplification and a lateral flow immunoassay (LFIA), namely an ATPL-based platform. The built-in workflow can isothermally complete a convenient and rapid detection of the target molecules with a minimum concentration of 2.46 copies/mL in about 20<!-- --> <!-- -->min, and markedly generate a lateral flow line signal for the naked eye. Moreover, a single-base difference can be distinguished from the target sequence, demonstrating high specificity and clinically analytical performance. Compared to the current common detection technology RT-qPCR, this platform provides detection results with high consistency with a kappa value of 0.927. In a word, the established ATPL-based detection platform is fast, highly sensitive and specific with promising accuracy, suggesting wide application potential in the detection of rotavirus or other nucleic acid in the future.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"34 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143661114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Many novel assays have been realized for the detection of antibiotics in food and environment. Low sensitivity and poor stability limit their application in electrochemical biosensors. The snowflake-like AuPd/Cu2S used in electrochemical sensor has a large area and high conductivity. Using it as an electrode modification material can effectively increase the working area of the electrode, thus further improving the current response. A more perfect snowflake-likeCu2S structure were synthesized after optimized for the substrate. After calculation it was surprisingly found that the two most prominent structures in each branch shape were divided into two parts by using them as the dividing line, which basically satisfied the golden section line ratio. Moreover, the modification of snowflake-like AuPd/Cu2S resulted in a significant increase in current by a factor of 1.48 compared to the bare electrode. Meanwhile, satellite-like Au/CeO2 loaded methylene blue (MB) was also used as a signal probe, which has a high and stable current signal. Based on the above, a kanamycin (Kana) detection method based on AuPd/Cu2S as a substrate and satellite-like Au/CeO2 as a signal probe to improve the sensitivity of electrochemical sensor was designed. Detection of Kana was accomplished by changes in target concentration combined with Exonuclease III (Exo Ⅲ) assisted double cycling. Under optimal conditions, the sensor had a detection range of 0.001-1000 ng/mL and a detection limit of 0.299 pg/mL. Repeatability, reproducibility, specificity, stability and Kana in real and quality control samples were successfully verified using the sensor with satisfactory results.
{"title":"“Superior snowflake” AuPd/Cu2S as substrate and satellite-like Au/CeO2 as signal probe to improve the sensitivity of electrochemical sensor for kanamycin detection","authors":"Jinmin Zhang, Zhiguang Suo, Min Wei, Hailin Shen, Xiaohui Chen, Huafen Wang, Yong Liu, Baoshan He, Huali Jin, Renyong Zhao","doi":"10.1016/j.snb.2025.137636","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137636","url":null,"abstract":"Many novel assays have been realized for the detection of antibiotics in food and environment. Low sensitivity and poor stability limit their application in electrochemical biosensors. The snowflake-like AuPd/Cu<sub>2</sub>S used in electrochemical sensor has a large area and high conductivity. Using it as an electrode modification material can effectively increase the working area of the electrode, thus further improving the current response. A more perfect snowflake-likeCu<sub>2</sub>S structure were synthesized after optimized for the substrate. After calculation it was surprisingly found that the two most prominent structures in each branch shape were divided into two parts by using them as the dividing line, which basically satisfied the golden section line ratio. Moreover, the modification of snowflake-like AuPd/Cu<sub>2</sub>S resulted in a significant increase in current by a factor of 1.48 compared to the bare electrode. Meanwhile, satellite-like Au/CeO<sub>2</sub> loaded methylene blue (MB) was also used as a signal probe, which has a high and stable current signal. Based on the above, a kanamycin (Kana) detection method based on AuPd/Cu<sub>2</sub>S as a substrate and satellite-like Au/CeO<sub>2</sub> as a signal probe to improve the sensitivity of electrochemical sensor was designed. Detection of Kana was accomplished by changes in target concentration combined with Exonuclease III (Exo Ⅲ) assisted double cycling. Under optimal conditions, the sensor had a detection range of 0.001-1000<!-- --> <!-- -->ng/mL and a detection limit of 0.299<!-- --> <!-- -->pg/mL. Repeatability, reproducibility, specificity, stability and Kana in real and quality control samples were successfully verified using the sensor with satisfactory results.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"106 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The 5-carboxylcytosine (5caC), an active DNA demethylation intermediate, is present in many cells and tissues and plays a key role in the regulation of gene expression. 5caC is highly similar in structure to 5-methylcytosine, 5-hydroxymethylcytosine and 5-formylcytosine, but its abundance is extremely low. To explore the biologic function of 5caC, the sensitive detection technique is required. To achieve this goal, a new photoelectrochemical (PEC) biosensor was developed for 5caC-DNA detection using Schottky junction integrated with type-I heterojunction of BiOCl/Bi2S3/CS-MXene tetany composite as photoactive material and 5caC-hairpin DNA hybridization triggered hybridization chain reaction (HCR) as signal amplification mode. Based on the double heterojunctions, the photoactivity of BiOCl/Bi2S3/CS-MXene improved greatly, leading to a high photocurrent response, and achieving high detection sensitivity. To perform HCR amplification, an ingenious hairpin DNA containing 5caC was designed to inhibit the cleavage activity of exonuclease III (Exo III). However, after hybridization with S1DNA, the unfolded hairpin DNA triggered the excision of double-stranded DNA to release the 5caC-DNA fragment, which further induced the HCR amplification and capture electron donor of methylene blue (MB) onto the electrode surface, causing an increased photocurrent response, and achieving the highly sensitive detection of 5cac-DNA and Exo III. The proposed PEC biosensor showed a detection range of 1 pM - 1 nM and 1 - 100 U/mL with detection limits of 0.16 pM and 0.34 U/mL (S/N=3) for 5caC DNA and Exo Ⅲ, respectively. The developed method can also be applied to screen Exo III inhibitor and evaluate the ecotoxic effect of pollutant.
{"title":"Photoelectrochemical biosensor for 5caC-DNA and Exo Ⅲ detection based on Schottky junction integrated with type-I heterojunction of BiOCl/Bi2S3/CS-MXene and hybridization chain reaction","authors":"Huanshun Yin, Yulin Zheng, Chengji Sui, Yunlei Zhou","doi":"10.1016/j.snb.2025.137647","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137647","url":null,"abstract":"The 5-carboxylcytosine (5caC), an active DNA demethylation intermediate, is present in many cells and tissues and plays a key role in the regulation of gene expression. 5caC is highly similar in structure to 5-methylcytosine, 5-hydroxymethylcytosine and 5-formylcytosine, but its abundance is extremely low. To explore the biologic function of 5caC, the sensitive detection technique is required. To achieve this goal, a new photoelectrochemical (PEC) biosensor was developed for 5caC-DNA detection using Schottky junction integrated with type-I heterojunction of BiOCl/Bi<sub>2</sub>S<sub>3</sub>/CS-MXene tetany composite as photoactive material and 5caC-hairpin DNA hybridization triggered hybridization chain reaction (HCR) as signal amplification mode. Based on the double heterojunctions, the photoactivity of BiOCl/Bi<sub>2</sub>S<sub>3</sub>/CS-MXene improved greatly, leading to a high photocurrent response, and achieving high detection sensitivity. To perform HCR amplification, an ingenious hairpin DNA containing 5caC was designed to inhibit the cleavage activity of exonuclease III (Exo III). However, after hybridization with <sub>S1</sub>DNA, the unfolded hairpin DNA triggered the excision of double-stranded DNA to release the 5caC-DNA fragment, which further induced the HCR amplification and capture electron donor of methylene blue (MB) onto the electrode surface, causing an increased photocurrent response, and achieving the highly sensitive detection of 5cac-DNA and Exo III. The proposed PEC biosensor showed a detection range of 1 pM - 1<!-- --> <!-- -->nM and 1 - 100<!-- --> <!-- -->U/mL with detection limits of 0.16 pM and 0.34<!-- --> <!-- -->U/mL (S/N=3) for 5caC DNA and Exo Ⅲ, respectively. The developed method can also be applied to screen Exo III inhibitor and evaluate the ecotoxic effect of pollutant.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"26 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-18DOI: 10.1016/j.snb.2025.137641
Jiani Yang, Ling Xia, Gongke Li
Nanocomposites-based nanozyme have been proposed to realize the combination of the respective properties of each component or to achieve cooperatively enhanced catalytic performances, but the systematic catalytic mechanism remains unclear in majority cases. Herein, an entirely new nanocomposite, amino nitrogen quantum dots hybrid platinum (aNDs/Pt hybrid) nanozyme was fabricated to reach a remarkable peroxidase-like activity owing to the synergetic effects between the two components. The roles of the oxygenous groups on the surface of aNDs/Pt hybrid determining their peroxidase-like catalytic activity were evaluated by regulating the functional groups during the synthesis of nitrogen quantum dots. Accordingly, empirical and theoretical evidences were given to identify the catalytic, substrate-binding, and inhibition sites in peroxidase-mimicking catalytic reactions of the aNDs/Pt hybrid. Meanwhile, the catalytic activity of aNDs/Pt hybrid nanozyme shows a strong dependence on the concentration of H2O2, based on which a highly sensitive fluorescent sensor for glucose detection in a microchip was established by employing aNDs/Pt hybrid as robust peroxidase. It is found that the developed sensor had excellent glucose sensing capability, including two-section broad linear detection ranges (0.1–6.0 μmol/L and 6.0–1000.0 μmol/L), less sample consumption (2.0 μL) and a low detection limit (31.7 nmol/L). Human serum and saliva samples were successfully examined by the as-proposed sensor with satisfactory results, showing the practical applications. This work not only gives a much deeper understanding on cooperatively catalytic mechanism for nanohybrids-based nanozymes, but also facilitate the design and fabrication of other types of target-specific artificial enzymes.
{"title":"Amino nitrogen quantum dots hybrid platinum as efficient peroxidase mimics for fluorescent sensing in microchip","authors":"Jiani Yang, Ling Xia, Gongke Li","doi":"10.1016/j.snb.2025.137641","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137641","url":null,"abstract":"Nanocomposites-based nanozyme have been proposed to realize the combination of the respective properties of each component or to achieve cooperatively enhanced catalytic performances, but the systematic catalytic mechanism remains unclear in majority cases. Herein, an entirely new nanocomposite, amino nitrogen quantum dots hybrid platinum (aNDs/Pt hybrid) nanozyme was fabricated to reach a remarkable peroxidase-like activity owing to the synergetic effects between the two components. The roles of the oxygenous groups on the surface of aNDs/Pt hybrid determining their peroxidase-like catalytic activity were evaluated by regulating the functional groups during the synthesis of nitrogen quantum dots. Accordingly, empirical and theoretical evidences were given to identify the catalytic, substrate-binding, and inhibition sites in peroxidase-mimicking catalytic reactions of the aNDs/Pt hybrid. Meanwhile, the catalytic activity of aNDs/Pt hybrid nanozyme shows a strong dependence on the concentration of H<sub>2</sub>O<sub>2</sub>, based on which a highly sensitive fluorescent sensor for glucose detection in a microchip was established by employing aNDs/Pt hybrid as robust peroxidase. It is found that the developed sensor had excellent glucose sensing capability, including two-section broad linear detection ranges (0.1–6.0 μmol/L and 6.0–1000.0 μmol/L), less sample consumption (2.0<!-- --> <!-- -->μL) and a low detection limit (31.7 nmol/L). Human serum and saliva samples were successfully examined by the as-proposed sensor with satisfactory results, showing the practical applications. This work not only gives a much deeper understanding on cooperatively catalytic mechanism for nanohybrids-based nanozymes, but also facilitate the design and fabrication of other types of target-specific artificial enzymes.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"34 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143653981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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