HPV integration profiling using nanopore sequencing and association with cervical precancerous lesion.

IF 4 2区生物学 Q2 MICROBIOLOGY Frontiers in Microbiology Pub Date : 2025-03-05 eCollection Date: 2025-01-01 DOI:10.3389/fmicb.2025.1522550

Ying Hou, Shoufeng Ni, Xin Liu, Xingyu Liu, Nan Wang, Fuqiang Xu, Jianyong Gao, Yanmei Li, Yuxiang Zhou, Huadong Tang, Meina Bian, Xiulan Li, Lili Zhang, Weiwei Wang, Qing Liu

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Abstract

Objectives: HPV infection and HPV DNA integration can lead to cervical cancer, but the relationship with lesion severity is unclear. This study aimed to investigate the correlation between HPV integration profile and cervical lesion extent.

Materials and methods: Twenty patients representing cervicitis, CIN I, CIN II, and CIN III underwent nanopore sequencing for HPV genotype and integration site analysis. HPV integration profiles were correlated with lesion severity. Gene Ontology (GO) and KEGG analysis were used to identify stage-specific genes and pathways.

Results: HPV integration rates were 60, 60, 100, and 100% for cervicitis, CIN I, CIN II, and CIN III, respectively, with varying numbers of integrated genes. Each group had specific stage-related genes, with 83 shared genes linked to neuron development and cell-cell processes. CIN II and CIN III displayed more cancer-related pathway enrichment than earlier stages.

Conclusion: A positive correlation exists between HPV integration frequency and cervical lesion stage. Late-stage lesions showed heightened enrichment in cancer-related pathways through specific HPV-integrated genes.

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来源期刊

Frontiers in Microbiology MICROBIOLOGY-

CiteScore

7.70

自引率

9.60%

发文量

4837

审稿时长

14 weeks

期刊介绍： Frontiers in Microbiology is a leading journal in its field, publishing rigorously peer-reviewed research across the entire spectrum of microbiology. Field Chief Editor Martin G. Klotz at Washington State University is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.