HPV integration profiling using nanopore sequencing and association with cervical precancerous lesion.

IF 4 2区生物学 Q2 MICROBIOLOGY Frontiers in Microbiology Pub Date : 2025-03-05 eCollection Date: 2025-01-01 DOI:10.3389/fmicb.2025.1522550

Ying Hou, Shoufeng Ni, Xin Liu, Xingyu Liu, Nan Wang, Fuqiang Xu, Jianyong Gao, Yanmei Li, Yuxiang Zhou, Huadong Tang, Meina Bian, Xiulan Li, Lili Zhang, Weiwei Wang, Qing Liu

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Abstract

Objectives: HPV infection and HPV DNA integration can lead to cervical cancer, but the relationship with lesion severity is unclear. This study aimed to investigate the correlation between HPV integration profile and cervical lesion extent.

Materials and methods: Twenty patients representing cervicitis, CIN I, CIN II, and CIN III underwent nanopore sequencing for HPV genotype and integration site analysis. HPV integration profiles were correlated with lesion severity. Gene Ontology (GO) and KEGG analysis were used to identify stage-specific genes and pathways.

Results: HPV integration rates were 60, 60, 100, and 100% for cervicitis, CIN I, CIN II, and CIN III, respectively, with varying numbers of integrated genes. Each group had specific stage-related genes, with 83 shared genes linked to neuron development and cell-cell processes. CIN II and CIN III displayed more cancer-related pathway enrichment than earlier stages.

Conclusion: A positive correlation exists between HPV integration frequency and cervical lesion stage. Late-stage lesions showed heightened enrichment in cancer-related pathways through specific HPV-integrated genes.

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使用纳米孔测序的HPV整合分析及其与宫颈癌前病变的关联。

目的：HPV感染和HPV DNA整合可导致宫颈癌，但与病变严重程度的关系尚不清楚。本研究旨在探讨HPV整合谱与宫颈病变程度的相关性。材料和方法：20例宫颈炎，CIN I， CIN II和CIN III患者进行了HPV基因型和整合位点分析的纳米孔测序。HPV整合谱与病变严重程度相关。基因本体（GO）和KEGG分析用于鉴定阶段特异性基因和途径。结果：宫颈炎、CIN I、CIN II和CIN III的HPV整合率分别为60%、60%、100%和100%，整合基因的数量不同。每一组都有特定的阶段相关基因，其中有83个与神经元发育和细胞-细胞过程相关的共享基因。CIN II和CIN III比早期阶段表现出更多的癌症相关途径富集。结论：HPV整合频率与宫颈病变分期呈正相关。晚期病变通过特异性hpv整合基因表现出癌症相关通路的高度富集。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Microbiology MICROBIOLOGY-

CiteScore

7.70

自引率

9.60%

发文量

4837

审稿时长

14 weeks

期刊介绍： Frontiers in Microbiology is a leading journal in its field, publishing rigorously peer-reviewed research across the entire spectrum of microbiology. Field Chief Editor Martin G. Klotz at Washington State University is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.