Glass slide evanescent wave fluorescence biosensor integrated coupling aspherical lens with a sensing range of over 20 mm for multi-wavelength operation

IF 1.9 4区 工程技术 Q3 MICROSCOPY Journal of microscopy Pub Date : 2025-03-20 DOI:10.1111/jmi.13405
Qun Dai, Yanjun Hu, Xingang Dai, Bowen Niu, Yuan Li, Guofang Fan
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Abstract

Evanescent wave fluorescence biosensor is a research hotspot in the field of biomedical detection due to high sensitivity and specificity. However, the fluorescence biosensor still shows small sensing range and high production cost. In this paper, a simple and cheap sub-millimetre thickness glass slide evanescent wave fluorescence biosensor integrated an aspherical coupling lens is proposed. The proposed sub-millimetre thickness glass slide fluorescence biosensor shows a sensing range of over 20 mm for multi-wavelength operation for the three materials of N-BK7, N-LAF21 and N-LASF41. This will be helpful for the evanescent wave fluorescence biosensor.

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玻璃载玻片倏逝波荧光生物传感器集成耦合非球面透镜,传感范围超过20毫米,可用于多波长操作。
倏逝波荧光生物传感器以其高灵敏度和特异性成为生物医学检测领域的研究热点。然而,荧光生物传感器仍然存在传感范围小、生产成本高等问题。本文提出了一种简单、廉价的集成非球面耦合透镜的亚毫米厚度玻片倏逝波荧光生物传感器。该亚毫米厚度的玻片荧光生物传感器对N-BK7、N-LAF21和N-LASF41三种材料的多波长工作的传感范围超过20 mm。这对瞬变波荧光生物传感器的研制有一定的帮助。
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来源期刊
Journal of microscopy
Journal of microscopy 工程技术-显微镜技术
CiteScore
4.30
自引率
5.00%
发文量
83
审稿时长
1 months
期刊介绍: The Journal of Microscopy is the oldest journal dedicated to the science of microscopy and the only peer-reviewed publication of the Royal Microscopical Society. It publishes papers that report on the very latest developments in microscopy such as advances in microscopy techniques or novel areas of application. The Journal does not seek to publish routine applications of microscopy or specimen preparation even though the submission may otherwise have a high scientific merit. The scope covers research in the physical and biological sciences and covers imaging methods using light, electrons, X-rays and other radiations as well as atomic force and near field techniques. Interdisciplinary research is welcome. Papers pertaining to microscopy are also welcomed on optical theory, spectroscopy, novel specimen preparation and manipulation methods and image recording, processing and analysis including dynamic analysis of living specimens. Publication types include full papers, hot topic fast tracked communications and review articles. Authors considering submitting a review article should contact the editorial office first.
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