The importance role of central proline to the antimicrobial potency and selectivity of indolicidin

IF 2.3 3区生物学 Q3 MICROBIOLOGY Archives of Microbiology Pub Date : 2025-03-20 DOI:10.1007/s00203-025-04299-y

Hang Ngo Thu, Hoa Ngo Van, Hai Bui Thi Phuong, Thinh Pham Duc, Khanh Hoang Van, Khoa Nguyen Vu, Tan Nguyen Van, Linh Dang Thuy, Le Minh Bui, Tung Truong Thanh, Huy Luong Xuan, Mao Can Van

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Abstract

The increasing prevalence of multidrug-resistant pathogens urged the development of new therapeutic strategies, and antimicrobial peptides (AMPs) have emerged as promising candidates. Indolicidin, a proline-rich AMP, is effective against a wide range of pathogens by penetrating the membrane to disrupt the cytoplasmic membrane or inhibit DNA synthesis. This study investigates the impact of replacing the central proline residue in Indolicidin with glycine (IND-7G), D-proline (IND-7DP), or lysine (IND-7K). Results show that both glycine and D-proline substitutions significantly reduced antimicrobial activity with lower hemolysis than the parent peptide. Besides, the analog having lysine substitution (IND-7K) slightly increased activity against E. coli and C. albicans but reduced potency against S. aureus, E. faecalis, and K. pneumoniae. The hemolytic activity of IND-7K remained comparable to that of Indolicidin. These findings demonstrated the essential role of proline in maintaining the antimicrobial efficacy of Indolicidin.

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来源期刊

Archives of Microbiology 生物-微生物学

CiteScore

4.90

自引率

3.60%

发文量

601

审稿时长

3 months

期刊介绍： Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.