Technical approaches for breath aldehyde biomarker detection and disease diagnosis: A review

IF 2.5 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS Analytical biochemistry Pub Date : 2025-03-19 DOI:10.1016/j.ab.2025.115841

Taha Kafili-Hajlari , Abdolhossein Naseri , Atefeh Ansarin , Farzaneh Rasoulzadeh

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Abstract

Exhaled breath analysis holds promise as a non-invasive approach for disease diagnosis. Aldehydes represent a class of volatile organic compounds with diagnostic potential as breath biomarkers for cancers and other conditions. However, aldehydes exist at low concentrations in breath and have stability challenges. This review summarizes recent studies on breath aldehyde analysis, focusing on sample collection methodology, analytical techniques implemented, and key findings regarding aldehyde alterations in disease. Breath collection methods examined include commercial bags, end-tidal sampling devices, condensates, and direct analysis. Analytical techniques evaluated gas chromatography, mass spectrometry, and microextraction approaches. Emerging microextraction and sensing technologies are advancing real-time, non-invasive aldehyde detection. Overall, breath aldehyde biomarkers offer immense potential for diagnosis and screening, but continued research is needed to address current limitations. This review provides insights to guide future efforts focused on exhaled aldehyde analysis and disease detection.

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呼吸醛生物标志物检测与疾病诊断技术途径综述

呼气分析有望成为一种非侵入性的疾病诊断方法。醛是一类具有诊断潜力的挥发性有机化合物，可作为癌症和其他疾病的呼吸生物标志物。然而，醛在呼吸中的浓度很低，并且具有稳定性方面的挑战。本文综述了呼吸醛分析的最新研究，重点介绍了样品采集方法、分析技术的实施以及有关疾病中醛变化的主要发现。所检查的呼气收集方法包括商业袋、潮末取样装置、冷凝物和直接分析。分析技术评估气相色谱法、质谱法和微萃取方法。新兴的微提取和传感技术正在推进实时、无创的醛检测。总的来说，呼吸醛生物标志物为诊断和筛查提供了巨大的潜力，但需要继续研究以解决当前的局限性。本文综述为指导今后呼气醛分析和疾病检测提供了参考。

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.