Sulfo-phospho-vanillin method for screening Aurantiochytrium strains with high docosahexaenoic acid levels.

IF 3.5 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY AMB Express Pub Date : 2025-03-20 DOI:10.1186/s13568-025-01859-9

Person Pesona Renta, Ta-Yu Huang, Ping-Hao Yu, Anna C-C Jang, Yi-Min Chen

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Abstract

Aurantiochytrium are protists found in marine and estuarine environments, known for producing high quantities of omega-3 fatty acids, particularly docosahexaenoic acid (DHA). The commercial viability of this species is currently hindered by the lack of reliable screening methods for the rapid identification of strains with high DHA content. This study developed a high-throughput screening platform based on the sulfo-phospho-vanillin (SPV) reaction, which produces a pink chromophore upon reacting with C-C double bonds in lipids. Analysis of 200 strains derived through the UV mutagenesis of the Aurantiochytrium limacinum strain BL10 revealed 7 strains that exhibited significantly elevated SPV reactivity, compared to the naïve strain (P < 0.01). Gas chromatography-mass spectrometry analysis revealed that in 4 of the 7 strains, DHA levels were significantly higher than those of the naïve strain. Among those 4 strains, the SPV reactivity values of two strains were precisely correlated with their DHA/DPA content. The SPV reaction protocol proposed in this paper has considerable potential for the high-throughput screening of Aurantiochytrium strains particularly those derived through UV mutagenesis.

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来源期刊

AMB Express BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

7.20

自引率

2.70%

发文量

141

审稿时长

13 weeks

期刊介绍： AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.