Reduction rate of14C-carmoisine by resting cell bacterial suspension from human and rat faeces

Pharmacological research communications Pub Date : 1988-10-01 DOI:10.1016/0031-6989(88)90007-0

L.M. Flaminio , E. Tragni , A. De Giorgi , T. Brusa , C.L. Galli

{"title":"Reduction rate of14C-carmoisine by resting cell bacterial suspension from human and rat faeces","authors":"L.M. Flaminio , E. Tragni , A. De Giorgi , T. Brusa , C.L. Galli","doi":"10.1016/0031-6989(88)90007-0","DOIUrl":null,"url":null,"abstract":"<div>14C-Carmoisine (250/gmg; 1.25 × 106 dpm) was incubated under strictly anaerobic conditions with resting cell suspension from stool specimens collected from male rats and human male healthy adults.The kinetics of azoreduction was determined as amount of naphthionic acid (NA), the stable metabolite of Carmoisine produced by the activity of the anaerobic bacteria. The analytical determinations were performed by radio-HPLC technique.There were no significant qualitative differences in the radiochro-matographic profiles of samples obtained from human and rat flora suspensions.The calculated reduction rates were 5.03 ± 0.18 nmoles of NA/250 /ug protein/min, and 1.72 ± 0.12 nmoles of NA/250 /ug protein/min for rat and human faecal resting cells respectively. From our results it seems that the enzymatic reaction, following zero order kinetics, is similar for the two species and only the reduction rate is different.</div>","PeriodicalId":19810,"journal":{"name":"Pharmacological research communications","volume":"20 10","pages":"Pages 907-916"},"PeriodicalIF":0.0000,"publicationDate":"1988-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0031-6989(88)90007-0","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacological research communications","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0031698988900070","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 3

Abstract

¹⁴C-Carmoisine (250/gmg; 1.25 × 10⁶ dpm) was incubated under strictly anaerobic conditions with resting cell suspension from stool specimens collected from male rats and human male healthy adults.

The kinetics of azoreduction was determined as amount of naphthionic acid (NA), the stable metabolite of Carmoisine produced by the activity of the anaerobic bacteria. The analytical determinations were performed by radio-HPLC technique.

There were no significant qualitative differences in the radiochro-matographic profiles of samples obtained from human and rat flora suspensions.

The calculated reduction rates were 5.03 ± 0.18 nmoles of NA/250 /ug protein/min, and 1.72 ± 0.12 nmoles of NA/250 /ug protein/min for rat and human faecal resting cells respectively. From our results it seems that the enzymatic reaction, following zero order kinetics, is similar for the two species and only the reduction rate is different.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

人类和大鼠粪便中静息细胞细菌悬浮液对14c -卡莫辛的还原率

14 c-carmoisine (250 / gmg;1.25 × 106 dpm)在严格厌氧条件下与雄性大鼠和人类健康男性成人粪便标本的静息细胞悬液孵育。氮还原的动力学是用萘硫酸(NA)的量来测定的，萘硫酸是由厌氧细菌活性产生的卡莫辛的稳定代谢物。采用放射性高效液相色谱法测定。从人和大鼠菌群悬浮液中获得的样品的放射色谱谱没有显著的定性差异。计算得到大鼠和人粪便静息细胞NA/250 /ug蛋白的还原率分别为5.03±0.18 nmol /min和1.72±0.12 nmol /250 /ug蛋白/min。从我们的结果看来，酶促反应，遵循零级动力学，是相似的两种，只是还原速率不同。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Pharmacological research communications

自引率

0.00%

发文量