Functional MRI and Tumor Vasculature Correlation in Ewing Sarcoma Xenografts: A Prospective Study Based on MRI–Pathology Co-Alignment

IF 3.5 2区医学 Q1 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING Journal of Magnetic Resonance Imaging Pub Date : 2025-03-20 DOI:10.1002/jmri.29770

Xiaoge Liu, Kai Zhang, Yutong Song, Xiyang Deng, Juan Tao, Yajie Liu, Chengjiang Xu, Guijiao Qin, Yasmin Mushtaq, Shaowu Wang

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Abstract

Background

Limited studies have evaluated vascular markers of Ewing sarcoma (ES) using MRI.

Purpose

To explore the correlation between tumor vascular markers and MRI perfusion parameters in ES xenografts based on MRI–pathology co-alignment.

Study Type

Prospective.

Animal Model

Thirty-four ES xenograft models were established in female athymic nude mice using the human-derived A673 cell line.

Field Strength/Sequence

3 T MRI, T₁-weighted (T1w) with fast spin echo sequence, T2w with fast recovery fast spin echo sequence, intravoxel incoherent motion (IVIM) with echo-planar diffusion-weighted sequence, and dynamic contrast-enhanced MRI (DCE-MRI) with the liver acquisition with volume acceleration sequence.

Assessment

IVIM parameters (D, D*, and f), DCE-MRI semiquantitative parameters (maximum slope of increase [MSI], contrast-enhancement ratio [CER], and initial area under the gadolinium curve [iAUGC]), and DCE-MRI quantitative parameters (K _trans, K _ep, and V _e). The expression of vascular endothelial growth factor (VEGF), microvessel density (MVD), and vascular mimicry (VM) was evaluated by immunohistochemical staining.

Statistical Tests

Intraclass correlation coefficient (ICC), bootstrap resampling, Fisher's Z transformation, Pearson or Spearman correlation analysis, receiver operating characteristic curve (ROC) analysis, and DeLong's test. p < 0.05 was considered statistically significant.

Results

K _trans, K _ep, f, and D* values showed significant correlations with VEGF (r = 0.697, 0.630, 0.781, 0.695, respectively). D*, f, K _trans, MSI, and CER values showed significant correlations with MVD (r = 0.42, 0.554, 0.486, 0.461, 0.416, respectively). D and f values showed significant correlations with VM (r = −0.552, 0.384, respectively). K _trans, f, D*, and K _ep values were good diagnostics in distinguishing between high- and low-expression groups of VEGF (AUC = 0.833–0.954). D* and D values were good diagnostics in distinguishing between high- and low-expression groups of MVD and VM (AUC = 0.727, 0.739, respectively).

Data Conclusion

IVIM and DCE-MRI can be utilized to assess tumor vasculature in ES xenografts.

Evidence Level

Technical Efficacy

Stage 3.

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尤文肉瘤异种移植物的功能性核磁共振成像与肿瘤血管的相关性：基于核磁共振成像与病理学共同配准的前瞻性研究

背景：有限的研究利用MRI评估尤文氏肉瘤（ES）的血管标志物。目的：探讨异种ES移植肿瘤血管标志物与MRI灌注参数的相关性。研究类型：前瞻性。动物模型：利用人源性A673细胞系建立34只雌性胸腺裸鼠ES异种移植模型。场强/序列：3t MRI， t1加权（T1w）快速自旋回波序列，T2w快速恢复快速自旋回波序列，体素内非相干运动（IVIM）回声平面扩散加权序列，动态对比增强MRI （DCE-MRI）肝脏采集与体积加速序列。评估方法：IVIM参数（D、D*、f）、DCE-MRI半定量参数（最大增加斜率[MSI]、对比增强比[CER]、初始造影曲线下面积[iAUGC]）、DCE-MRI定量参数（Ktrans、Kep、Ve）。免疫组化染色评价血管内皮生长因子（VEGF）表达、微血管密度（MVD）和血管拟态（VM）。统计检验：类内相关系数（ICC）、自举重抽样、Fisher’s Z变换、Pearson或Spearman相关分析、受试者工作特征曲线（ROC）分析、DeLong检验。结果：Ktrans、Kep、f、D*值与VEGF呈显著相关（r分别为0.697、0.630、0.781、0.695）。D*、f、Ktrans、MSI、CER值与MVD呈极显著相关（r分别为0.42、0.554、0.486、0.461、0.416）。D、f值与VM呈显著相关（r分别为-0.552、0.384）。Ktrans、f、D*、Kep值是区分VEGF高、低表达组较好的诊断指标（AUC = 0.833-0.954）。D*和D值是区分MVD和VM高表达组和低表达组的良好诊断指标（AUC分别为0.727、0.739）。结论：IVIM和DCE-MRI可用于ES异种移植物肿瘤血管的评估。证据等级：1。技术功效：第3阶段。

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来源期刊

Journal of Magnetic Resonance Imaging 医学-核医学

CiteScore

9.70

自引率

6.80%

发文量

494

审稿时长

2 months

期刊介绍： The Journal of Magnetic Resonance Imaging (JMRI) is an international journal devoted to the timely publication of basic and clinical research, educational and review articles, and other information related to the diagnostic applications of magnetic resonance.