Apoplastic barriers are essential for nodule formation and nitrogen fixation in Lotus japonicus

IF 45.8 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES Science Pub Date : 2025-03-20 DOI:10.1126/science.ado8680

Defeng Shen, Nikola Micic, Rafael E. Venado, Nanna Bjarnholt, Christoph Crocoll, Daniel Pergament Persson, Sebastian Samwald, Stanislav Kopriva, Philip Westhoff, Sabine Metzger, Ulla Neumann, Ryohei Thomas Nakano, Macarena Marín Arancibia, Tonni Grube Andersen

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Abstract

Establishment of the apoplastic root barrier known as the Casparian strip occurs early in root development. In legumes, this area overlaps with nitrogen-fixing nodule formation, which raises the possibility that nodulation and barrier formation are connected. Nodules also contain Casparian strips, yet, in this case, their role is unknown. We established mutants with defective barriers in Lotus japonicus. This revealed that effective apoplastic blockage in the endodermis is important for root-to-shoot signals underlying nodulation. Our findings further revealed that in nodules, the genetic machinery for Casparian strip formation is shared with roots. Apoplastic blockage controls the metabolic source-sink status required for nitrogen fixation. This identifies Casparian strips as a model system to study spatially constrained symbiotic plant-microbe relationships.

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外胞质屏障对荷花根瘤形成和固氮至关重要。

在根发育早期，被称为卡斯帕里斯带的外体根屏障就已经建立起来了。在豆科植物中，这个区域与固氮结瘤形成重叠，这增加了结瘤和屏障形成联系的可能性。结节也含有卡氏带，然而，在这种情况下，它们的作用是未知的。我们在日本莲中建立了具有缺陷屏障的突变体。这表明，有效的内胚层外胞体阻断对于根瘤形成的根尖信号是重要的。我们的研究结果进一步表明，在根瘤中，Casparian条带形成的遗传机制与根共享。外胞体阻塞控制着固氮所需的代谢源库状态。这确定了Casparian条带作为研究空间约束的共生植物-微生物关系的模型系统。

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Science 综合性期刊-综合性期刊

CiteScore

61.10

自引率

0.90%

发文量

审稿时长

2.1 months

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