Apoplastic barriers are essential for nodule formation and nitrogen fixation in Lotus japonicus.

IF 44.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES Science Pub Date : 2025-03-21 Epub Date: 2025-03-20 DOI:10.1126/science.ado8680

Defeng Shen, Nikola Micic, Rafael E Venado, Nanna Bjarnholt, Christoph Crocoll, Daniel Pergament Persson, Sebastian Samwald, Stanislav Kopriva, Philip Westhoff, Sabine Metzger, Ulla Neumann, Ryohei Thomas Nakano, Macarena Marín Arancibia, Tonni Grube Andersen

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Abstract

Establishment of the apoplastic root barrier known as the Casparian strip occurs early in root development. In legumes, this area overlaps with nitrogen-fixing nodule formation, which raises the possibility that nodulation and barrier formation are connected. Nodules also contain Casparian strips, yet, in this case, their role is unknown. We established mutants with defective barriers in Lotus japonicus. This revealed that effective apoplastic blockage in the endodermis is important for root-to-shoot signals underlying nodulation. Our findings further revealed that in nodules, the genetic machinery for Casparian strip formation is shared with roots. Apoplastic blockage controls the metabolic source-sink status required for nitrogen fixation. This identifies Casparian strips as a model system to study spatially constrained symbiotic plant-microbe relationships.

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Science 综合性期刊-综合性期刊

CiteScore

61.10

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0.90%

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审稿时长

2.1 months

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