Characterization and comparison of Schizosaccharomyces pombe cdc15 temperature-sensitive mutants.

Abstract

The F-BAR protein Cdc15 is essential for cytokinesis in the fission yeast Schizosaccharomyces pombe , playing a key scaffolding role and connecting the actomyosin-based cytokinetic ring to the plasma membrane. Here, we compared cdc15 temperature-sensitive mutants isolated in multiple genetic screens. We determined the mutations within each cdc15 mutant allele and analyzed their growth at different temperatures. Additionally, we report a new cdc15 allele that highlights the requirement for Cdc15 in the recruitment of the early secretory pathway to the cellular division site. The new mutants described here expand the toolkit for studying cytokinesis in S. pombe .