DNA Dual-Color Probes for Real-Time Monitoring of p21 mRNA and Bax mRNA during Apoptosis in Breast Cancer Cells

IF 8.3 2区材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY ACS Applied Materials & Interfaces Pub Date : 2025-03-22 DOI:10.1021/acsami.5c01929

Xiao Qiao Wu, Chun Tian, Yiaobo Zhang, Rui Liang, Lin Liu, Kun Han Nie, Qian Kai Lin, Jing Li, Cheng Zhi Huang, Chun Mei Li

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Abstract

Monitoring apoptosis in breast cancer cells is crucial for understanding the molecular mechanisms underlying breast cancer and evaluating the therapeutic effects of anticancer drugs. Real-time monitoring of p21 mRNA and Bax mRNA during apoptosis offers a valuable tool for assessing drug efficacy. Tetrahedral DNA frameworks (TDFs) are known for their ease of synthesis and multiple signal outputs. Leveraging these properties, this study developed DNA dual-color probes (DDCPs) for the real-time monitoring of apoptosis in breast cancer cells. In the presence of p21 and Bax mRNA, strand displacement reactions within the DDCPs restore Cy5 and FAM fluorescence signals, respectively. This strategy is consistent with the results of RT-qPCR studies. Moreover, the DDCPs allow for the specific investigation of apoptotic pathways in breast cancer cells influenced by different drugs by using distinct fluorescent signals for pathway differentiation. This method holds promise as a powerful tool for investigating apoptotic mechanisms in breast cancer cells, uncovering associated signaling pathways, and screening potential therapeutic drugs.

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来源期刊

ACS Applied Materials & Interfaces 工程技术-材料科学：综合

CiteScore

16.00

自引率

6.30%

发文量

4978

审稿时长

1.8 months

期刊介绍： ACS Applied Materials & Interfaces is a leading interdisciplinary journal that brings together chemists, engineers, physicists, and biologists to explore the development and utilization of newly-discovered materials and interfacial processes for specific applications. Our journal has experienced remarkable growth since its establishment in 2009, both in terms of the number of articles published and the impact of the research showcased. We are proud to foster a truly global community, with the majority of published articles originating from outside the United States, reflecting the rapid growth of applied research worldwide.