Rapid and Sensitive Determination of Vancomycin by AIE-Active Fluorescent Probe for Clinical Monitoring

Abstract

Vancomycin (Van) is the preferred drug for clinically treating severe infections caused by Gram-positive bacteria, especially in intensive care unit (ICU). However, due to safety concerns, strict management and administration of Van are required in clinical practice. Hence, there is a need to develop a rapid and accurate method for determining the concentration of Van. In this study, a cyanostilbene-based fluorescent probe, Cy-KAA, is designed for the rapid, highly sensitive and selective detection of Van. In the probe, L-Lys-D-Ala-D-Ala polypeptide moiety can interact with Van, resulting in a rapid fluorescence response that reaches a plateau within 2 minutes. Cy-KAA can be employed for the detection of Van in clinical ICU samples, demonstrating strong correlation with the traditional HPLC method (consistency up to 0.99). These findings highlight the high accuracy and potential utility of Cy-KAA as a powerful tool for real-time monitoring of Van levels in clinical practice, thereby providing valuable guidance for precise drug administration.