Xiaoqi Hu , Kiseok Han , Gyuho Choi , Myungeun Suk , Tae-Jin Kim
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引用次数: 0
Abstract
Fluorescence resonance energy transfer (FRET)-based live-cell imaging and genetically encoded biosensors are widely used to study biochemical processes in living cells. However, there remains a gap in robust analytical methods for examining complex processes like endosomal trafficking. In this study, we introduce two advanced tools—EasyFRET and EndoAna—specifically designed for FRET image analysis, along with two novel potassium biosensors, GalT-KIRIN and KIRIN-Lamp1, targeted to endosomes and lysosomes, respectively. Together, these tools establish a comprehensive workflow for investigating potassium signaling, which plays a critical role in cellular homeostasis and various biological functions. Our live-cell imaging results demonstrate that EasyFRET and EndoAna provide efficient and reliable FRET image analysis, while the potassium indicators offer valuable insights into potassium dynamics within the endosomal trafficking system.
期刊介绍：
Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.
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