Altered brain-derived neurotrophic factor levels and oxidative stress in REM sleep deprivation: a rat model study.

IF 2.2 3区医学 Q3 CLINICAL NEUROLOGY BMC Neurology Pub Date : 2025-03-21 DOI:10.1186/s12883-025-04127-2

Yonca Coluk, Guven Yildirim, Sembol Yildirmak, Emine Gulceri Gulec Peker

{"title":"Altered brain-derived neurotrophic factor levels and oxidative stress in REM sleep deprivation: a rat model study.","authors":"Yonca Coluk, Guven Yildirim, Sembol Yildirmak, Emine Gulceri Gulec Peker","doi":"10.1186/s12883-025-04127-2","DOIUrl":null,"url":null,"abstract":"Background: Brain-derived neurotrophic factor (BDNF) is among the modulators associated with cognition and sleep that play a role in sleep disorders. This study aimed at investigating the effects of chronic sleep deprivation and REM sleep deprivation on BDNF levels and oxidative stress markers.Methods: A total of 24 healthy male Wistar albino rats were separated into 3 groups as REM sleep deprivation group, control sleep deprivation group and control group. To create models of 21-day REM sleep deprivation and control sleep deprivation, we used the platform technique. After 21 days blood BDNF, brain tissue BDNF, brain tissue malondialdehyde, glutathione, ascorbic acid, nitrite and nitrate were evaluated.Results: Compared with the control group, control sleep deprivation group showed a significant increase in brain tissue levels of BDNF (p = 0.038), whereas a significant decrease was observed in the levels of glutathione (GSH) and nitric oxide (NO) (p:0.036). No statistical difference was observed between the blood levels of BDNF in either group (p: 0.795).Conclusion: Our results showed decreases in GSH and NO levels and increases in malondialdehyde levels in the sleep deprivation models, reflecting oxidative stress in the brain. Additionally, we observed increases in brain BDNF levels in the control sleep deprivation model.","PeriodicalId":9170,"journal":{"name":"BMC Neurology","volume":"25 1","pages":"122"},"PeriodicalIF":2.2000,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11927282/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Neurology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s12883-025-04127-2","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CLINICAL NEUROLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Brain-derived neurotrophic factor (BDNF) is among the modulators associated with cognition and sleep that play a role in sleep disorders. This study aimed at investigating the effects of chronic sleep deprivation and REM sleep deprivation on BDNF levels and oxidative stress markers.

Methods: A total of 24 healthy male Wistar albino rats were separated into 3 groups as REM sleep deprivation group, control sleep deprivation group and control group. To create models of 21-day REM sleep deprivation and control sleep deprivation, we used the platform technique. After 21 days blood BDNF, brain tissue BDNF, brain tissue malondialdehyde, glutathione, ascorbic acid, nitrite and nitrate were evaluated.

Results: Compared with the control group, control sleep deprivation group showed a significant increase in brain tissue levels of BDNF (p = 0.038), whereas a significant decrease was observed in the levels of glutathione (GSH) and nitric oxide (NO) (p:0.036). No statistical difference was observed between the blood levels of BDNF in either group (p: 0.795).

Conclusion: Our results showed decreases in GSH and NO levels and increases in malondialdehyde levels in the sleep deprivation models, reflecting oxidative stress in the brain. Additionally, we observed increases in brain BDNF levels in the control sleep deprivation model.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

快速眼动睡眠剥夺时脑源性神经营养因子水平和氧化应激的改变：一项大鼠模型研究。

背景：脑源性神经营养因子（BDNF）是与认知和睡眠相关的调节因子之一，在睡眠障碍中发挥作用。本研究旨在探讨慢性睡眠剥夺和快速眼动睡眠剥夺对BDNF水平和氧化应激标志物的影响。方法：将24只健康雄性Wistar白化大鼠分为3组，分别为REM睡眠剥夺组、对照组和对照组。为了建立21天快速眼动睡眠剥夺模型和控制睡眠剥夺，我们使用了平台技术。21 d后测定血BDNF、脑组织BDNF、脑组织丙二醛、谷胱甘肽、抗坏血酸、亚硝酸盐和硝酸盐。结果：与对照组相比，睡眠剥夺对照组脑组织BDNF水平显著升高（p = 0.038），而谷胱甘肽（GSH）和一氧化氮（NO）水平显著降低（p:0.036）。两组血中BDNF水平无统计学差异（p: 0.795）。结论：我们的研究结果显示，在睡眠剥夺模型中，GSH和NO水平降低，丙二醛水平升高，反映了大脑的氧化应激。此外，我们观察到在睡眠剥夺控制模型中大脑BDNF水平的增加。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

BMC Neurology 医学-临床神经学

CiteScore

4.20

自引率

0.00%

发文量

428

审稿时长

3-8 weeks

期刊介绍： BMC Neurology is an open access, peer-reviewed journal that considers articles on all aspects of the prevention, diagnosis and management of neurological disorders, as well as related molecular genetics, pathophysiology, and epidemiology.