Krüppel homolog 1, a juvenile hormone transcription factor, regulates the reproduction and development of Aedes albopictus(Skuse) (Diptera: Culicidae).

IF 2 3区医学 Q2 PARASITOLOGY Parasitology Research Pub Date : 2025-03-22 DOI:10.1007/s00436-025-08479-z

Ya-Hui Chen, Cheng Wu, Yu-Yang Xie, Yan-Hui Zhang, Xi-Tong Huang, Fen Hu, Li-Hua Xie

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Abstract

The control of Aedes albopictus (Skuse) (Diptera: Culicidae), a vector for several important viral diseases, is crucial for mitigating mosquito-borne diseases. This study focused on the Krüppel homolog 1 (Kr-h1) gene, a transcription factor in juvenile hormone (JH) signaling, which plays a pivotal role in inhibiting metamorphosis and promoting adult reproduction. We characterized Aedes albopictus Kr-h1(AalbKr-h1), identified its eight zinc finger domains, and confirmed its orthology among insects through phylogenetic analysis. Expression profiling across life stages showed high level of expression in eggs, late larvae, and adults, with minimal expression in pupae. In adults, AalbKr-h1 was most active in the fat body and ovaries. Exposing larvae to a JH analogue significantly upregulated AalbKr-h1 expression in both larval and adult stages. RNAi-mediated knockdown of Kr-h1 protein reduced egg reproduction, survival rate and gene expression levels. These results provide a solid foundation for further exploration of the function of the AalbKr-h1 gene and the potential development of novel strategies for mosquito control and prevention of mosquito-borne diseases.

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kr ppel同源物1是调节白纹伊蚊（Skuse）（双翅目：库蚊科）繁殖发育的一种幼体激素转录因子。

白纹伊蚊（Skuse）（双翅目：库蚊科）是几种重要病毒性疾病的媒介，控制白纹伊蚊对减轻蚊媒疾病至关重要。kr ppel同源物1 （Kr-h1）基因是幼体激素（JH）信号转导的转录因子，在抑制变态和促进成体繁殖中起关键作用。我们对白纹伊蚊Kr-h1（AalbKr-h1）进行了特征鉴定，鉴定了其8个锌指结构域，并通过系统发育分析确定了其在昆虫中的同源性。跨生命阶段的表达谱显示，卵、晚期幼虫和成虫的表达水平较高，蛹的表达水平最低。在成人中，AalbKr-h1在脂肪体和卵巢中最活跃。暴露于JH类似物的幼虫在幼虫期和成虫期都显著上调了AalbKr-h1的表达。rnai介导的Kr-h1蛋白敲低降低了卵子的繁殖、存活率和基因表达水平。这些结果为进一步探索AalbKr-h1基因的功能和开发蚊虫控制和预防蚊媒疾病的新策略提供了坚实的基础。

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来源期刊

Parasitology Research 医学-寄生虫学

CiteScore

4.10

自引率

5.00%

发文量

346

审稿时长

6 months

期刊介绍： The journal Parasitology Research covers the latest developments in parasitology across a variety of disciplines, including biology, medicine and veterinary medicine. Among many topics discussed are chemotherapy and control of parasitic disease, and the relationship of host and parasite. Other coverage includes: Protozoology, Helminthology, Entomology; Morphology (incl. Pathomorphology, Ultrastructure); Biochemistry, Physiology including Pathophysiology; Parasite-Host-Relationships including Immunology and Host Specificity; life history, ecology and epidemiology; and Diagnosis, Chemotherapy and Control of Parasitic Diseases.