Lipid-lowering effects and metabolomic investigation of Polygonatum sibiricum fermented by Lactobacillus plantarum NX-1

Abstract

In this study, Lactobacillus plantarum NX-1 was utilized to ferment Polygonatum sibiricum. Through optimization of this process, the inhibition rate of pancreatic lipase was enhanced by 163.2 %. Further metabolomic analysis showed that the content of cholic acid metabolites such as taurocholic acid, acetylcholine, phosphocholine, linoleic acid metabolites, (+/-)12, 13-DiHOME and 13-OxoODE increased observably after fermentation, which was an important reason for its lipid-lowering effect. In the high-fat zebrafish model, the fermented Polygonatum sibiricum significantly inhibited lipid accumulation. In summary, Lactobacillus plantarum NX-1 fermentation not only increases the contents of polysaccharides and saponins in polygonatum sibiricum extract but also significantly increases the contents of taurocholic acid, acetylcholine, phosphocholine, (+/-)12, 13-DiHOME and 13-OxoODE. In addition, it can inhibit the lipid accumulation of the high-fat diet-fed zebrafish.