Effects of electrical stimulation of the lateral cerebellar nucleus on PTZ-kindled seizures

IF 2.3 3区医学 Q2 BEHAVIORAL SCIENCES Epilepsy & Behavior Pub Date : 2025-06-01 Epub Date: 2025-03-22 DOI:10.1016/j.yebeh.2025.110377

Leonid S. Godlevsky , Mykhailo P. Pervak , Olha S. Yehorenko , Serhii V. Marchenko

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Abstract

Background

In recent years, the cerebellum and its nuclei have become an essential target for understanding and suppressing the mechanisms of seizures. This study aimed to investigate the effects of electrical stimulation (ES) applied to the Lateral Cerebellar Nucleus (LCN) in rats at the early and fully developed pentylenetetrazol (PTZ) kindled seizures.

Methods

The experimental groups were represented by the male Wistar rats kindled with PTZ (35.0 mg/kg, i.p.) to myoclonus (9–11 PTZ injections) and generalized tonic-clonic seizures (21 PTZ injections). Unilateral ES (100 Hz) was delivered daily for five days after the last kindled PTZ administration, with PTZ seizure testing after ES. Seizures were videotaped, and the severity score was determined in a blinded manner.

Results

ES of LCN performed at the early stage of kindling facilitated the appearance of myoclonus, and increased seizure severity by 30.2 % points compared to the control group (H = 6.94; df = 2; p = 0.037) with the spikes frequency generation increased during the poststimulation period (H = 27.34; df = 5; p < 0.001). In fully developed kindling, ES prevented generalized seizure and reduced seizure severity by 27.5 % (H = 9.385; df = 2; p = 0.009), while myoclonuses were present with spikes generation in brain structures.

Conclusion

The data obtained showed that repeated ES of LCN at the early stage promoted myoclonic seizures, while in fully PTZ-kindled rats, it suppressed generalized seizure fits, which were substituted with myoclonus.

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电刺激小脑外侧核对ptz点燃癫痫发作的影响

近年来，小脑及其核已成为了解和抑制癫痫发作机制的重要靶点。本研究旨在探讨电刺激（ES）对早期和完全发作的大鼠小脑外侧核（LCN）的影响。方法以PTZ （35.0 mg/kg, ig）致肌阵挛（9 ~ 11次PTZ注射）和全身性强直阵挛发作（21次PTZ注射）雄性Wistar大鼠为实验组。在最后一次点燃PTZ后的五天内，每天进行单侧ES (100 Hz)，并在ES后进行PTZ发作测试。对癫痫发作进行录像，并以盲法确定严重程度评分。结果点火早期进行LCN，使肌阵挛的出现更容易，发作严重程度较对照组提高30.2% (H = 6.94；df = 2；p = 0.037)，刺激后尖峰频率增加(H = 27.34；df = 5；p & lt;0.001)。在完全发育的点火实验中，ES预防了全身癫痫发作，并将癫痫发作严重程度降低了27.5% (H = 9.385；df = 2；P = 0.009)，而肌阵挛存在，脑结构产生尖峰。结论早期LCN反复发作可促进肌阵挛性发作，而在完全ptz点燃的大鼠中，可抑制全身性发作，取而代之的是肌阵挛性发作。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Epilepsy & Behavior 医学-行为科学

CiteScore

5.40

自引率

15.40%

发文量

385

审稿时长

43 days

期刊介绍： Epilepsy & Behavior is the fastest-growing international journal uniquely devoted to the rapid dissemination of the most current information available on the behavioral aspects of seizures and epilepsy. Epilepsy & Behavior presents original peer-reviewed articles based on laboratory and clinical research. Topics are drawn from a variety of fields, including clinical neurology, neurosurgery, neuropsychiatry, neuropsychology, neurophysiology, neuropharmacology, and neuroimaging. From September 2012 Epilepsy & Behavior stopped accepting Case Reports for publication in the journal. From this date authors who submit to Epilepsy & Behavior will be offered a transfer or asked to resubmit their Case Reports to its new sister journal, Epilepsy & Behavior Case Reports.