A novel disposable dual-sensing platform based on DNA-aptamer amplified with gold nanoparticles/Nb4C3-MXene for simultaneous detection of lead and cadmium

IF 21.8 2区材料科学 Q1 MATERIALS SCIENCE, COMPOSITES Advanced Composites and Hybrid Materials Pub Date : 2025-03-25 DOI:10.1007/s42114-025-01216-1

Hassan Karimi-Maleh, Zhouxiang Zhang, Najmeh Zare, Onur Karaman, Yangpin Wen, Tao Wu, Nianbing Zhong, Li Fu

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Abstract

Herein, we designed a special screen-printing carbon electrode system with two independent zones to realize the immobilization of two kinds of aptamers on electrode surface. Nb₄C₃-MXene is a remarkable member from MX₃ MXene with many excellent properties. In this study, Nb₄C₃-MXene nanosheets were firstly modified onto the screen-printing carbon electrode surface as the substrate materials to offer big surface area and then gold nanoparticles were loaded onto the surface of Nb₄C₃-MXene nanosheets through electrodeposition. Afterward, the aptamer-containing double-stranded DNA was spontaneously assembled onto the modified electrode surface through the Au–S bond. Owing to high affinity of aptamers towards the heavy metal ions (Cd²⁺ and Pb²⁺ in this case), the aptamers tagged with methylene blue and ferrocene would specifically bond with heavy metal ions to form folded structures and competed off from the electrode surface, and then the change of electrochemical signals can be detected by square wave voltammetry. The aptasensor exhibits a good linear response towards Cd²⁺ and Pb²⁺ from 1 × 10⁻¹⁰ to 1 × 10⁻⁷ M, and their detection limits are 59.8 pM of Pb²⁺ and Cd²⁺ of 146.2 pM; LOQ are 93.7 pM of Pb²⁺ and 164.8 pM of Cd²⁺, respectively.

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基于金纳米颗粒/Nb4C3-MXene扩增dna适配体的新型一次性双传感平台用于铅和镉的同时检测

为此，我们设计了一种特殊的丝网印刷碳电极系统，该系统具有两个独立的区域，可以实现两种适配体在电极表面的固定。Nb4C3-MXene是MX3 MXene的一个重要成员，具有许多优异的性能。本研究首先将Nb4C3-MXene纳米片作为衬底材料修饰在丝网印刷碳电极表面，以提供较大的比表面积，然后通过电沉积将金纳米颗粒负载在Nb4C3-MXene纳米片表面。随后，含有适配体的双链DNA通过Au-S键自发地组装到修饰的电极表面。由于适体对重金属离子（Cd2+和Pb2+）具有较高的亲和力，亚甲基蓝和二茂铁标记的适体会与重金属离子特异性结合形成折叠结构并从电极表面竞争，然后通过方波伏安法检测电化学信号的变化。该传感器对Cd2+和Pb2+在1 × 10−10 ~ 1 × 10−7 M范围内具有良好的线性响应，Pb2+和Cd2+的检出限分别为59.8 pM和146.2 pM；LOQ分别为Pb2+的93.7 pM和Cd2+的164.8 pM。

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来源期刊

Advanced Composites and Hybrid Materials Multiple-

CiteScore

26.00

自引率

21.40%

发文量

185

期刊介绍： Advanced Composites and Hybrid Materials is a leading international journal that promotes interdisciplinary collaboration among materials scientists, engineers, chemists, biologists, and physicists working on composites, including nanocomposites. Our aim is to facilitate rapid scientific communication in this field. The journal publishes high-quality research on various aspects of composite materials, including materials design, surface and interface science/engineering, manufacturing, structure control, property design, device fabrication, and other applications. We also welcome simulation and modeling studies that are relevant to composites. Additionally, papers focusing on the relationship between fillers and the matrix are of particular interest. Our scope includes polymer, metal, and ceramic matrices, with a special emphasis on reviews and meta-analyses related to materials selection. We cover a wide range of topics, including transport properties, strategies for controlling interfaces and composition distribution, bottom-up assembly of nanocomposites, highly porous and high-density composites, electronic structure design, materials synergisms, and thermoelectric materials. Advanced Composites and Hybrid Materials follows a rigorous single-blind peer-review process to ensure the quality and integrity of the published work.