A novel coumarin-incorporated lanthanide coordination nanoprobe for ratiometric sensing of tetracycline

Abstract

Tetracycline (Tc), a broad-spectrum antibiotic for treating bacterial infections, poses significant risks to human health and the environment. This study presents a novel lanthanide coordination probe, AMP/Eu/CMP, for the ratiometric detection of Tc. The pyridine-appended coumarin derivative, CMP, acting as a stable internal reference, combines with AMP and Eu³⁺ to form the robust ratiometric probe AMP/Eu/CMP. Upon binding to Tc, Eu³⁺ fluorescence (emission at 616 nm) is sensitized while CMP fluorescence (emission at 505 nm) remains unchanged, resulting in a clear fluorescence shift from blue-green to red, enabling effective ratiometric detection of Tc. By integrating a smartphone color recognition app, rapid and visual detection of tetracycline concentrations is achieved. Additionally, paper-based test strips were developed for on-site Tc detection, exhibiting a linear response across a wide concentration range, making this method suitable for applications in food safety, pharmaceutical analysis, and environmental monitoring. The use of a fluorescent molecule with unique photophysical properties as an internal reference enables the construction of a high-performance, ratiometric lanthanide coordination polymer probe that is rapid, simple, and cost-effective, providing valuable insights for the development of future fluorescence sensors.