Matthew K Schnizlein, Abhishek A Dubey, Aretha Fiebig, Sean Crosson
{"title":"Genetic- and culture-based tools for studying <i>Bacteroides fragilis</i>.","authors":"Matthew K Schnizlein, Abhishek A Dubey, Aretha Fiebig, Sean Crosson","doi":"10.1128/mra.00006-25","DOIUrl":null,"url":null,"abstract":"<p><p>The relatively limited availability of genetic tools has hampered mechanistic studies of <i>Bacteroides fragilis,</i> an opportunistic anaerobe that constitutes 1%-5% of the gut microbiota in healthy humans. Here we describe novel vectors for <i>B. fragilis</i> gene deletion and expression as well as a semi-defined media for cultivation of <i>B. fragilis</i> str. P207.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0000625"},"PeriodicalIF":0.7000,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12060699/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbiology Resource Announcements","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1128/mra.00006-25","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/3/25 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The relatively limited availability of genetic tools has hampered mechanistic studies of Bacteroides fragilis, an opportunistic anaerobe that constitutes 1%-5% of the gut microbiota in healthy humans. Here we describe novel vectors for B. fragilis gene deletion and expression as well as a semi-defined media for cultivation of B. fragilis str. P207.
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