{"title":"Simultaneous single-cell DNA damage profiling and RNA sequencing by Paired-Damage-seq","authors":"","doi":"10.1038/s41592-025-02634-1","DOIUrl":null,"url":null,"abstract":"We developed Paired-Damage-seq to profile oxidative DNA damage and single-stranded DNA damage jointly with gene-expression analysis in single cells. By dissecting cell-type-specific genome vulnerability, our approach provides a method to pinpoint hotspots of molecular fidelity loss during dynamic biological processes.","PeriodicalId":18981,"journal":{"name":"Nature Methods","volume":"22 5","pages":"904-905"},"PeriodicalIF":32.1000,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nature Methods","FirstCategoryId":"99","ListUrlMain":"https://www.nature.com/articles/s41592-025-02634-1","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
We developed Paired-Damage-seq to profile oxidative DNA damage and single-stranded DNA damage jointly with gene-expression analysis in single cells. By dissecting cell-type-specific genome vulnerability, our approach provides a method to pinpoint hotspots of molecular fidelity loss during dynamic biological processes.
期刊介绍：
Nature Methods is a monthly journal that focuses on publishing innovative methods and substantial enhancements to fundamental life sciences research techniques. Geared towards a diverse, interdisciplinary readership of researchers in academia and industry engaged in laboratory work, the journal offers new tools for research and emphasizes the immediate practical significance of the featured work. It publishes primary research papers and reviews recent technical and methodological advancements, with a particular interest in primary methods papers relevant to the biological and biomedical sciences. This includes methods rooted in chemistry with practical applications for studying biological problems.
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