Comparison of three extraction methods, DMU-SPME, SAFE, and SDE, for the analysis of flavor compounds in soy sauce

IF 2.8 3区 医学 Q2 BIOCHEMICAL RESEARCH METHODS Journal of Chromatography B Pub Date : 2025-03-22 DOI:10.1016/j.jchromb.2025.124562
Yun-Jiao Ma , An-Qi Bi , Ping Li , Bei-Wei Zhu , Ming Du , Xian-Bing Xu
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Abstract

The dual mode unity solid-phase microextraction (DMU-SPME) was evaluated against traditional solvent-based methods, including solvent-assisted flavor evaporation (SAFE) and simultaneous distillation extraction (SDE), to explore its potential as an alternative. Compared to SAFE and SDE, DMU-SPME is a green extraction technology for volatile organic compounds. The results demonstrated that DMU-SPME significantly outperformed both SAFE and SDE in extracting medium-volatility compounds (retention time of 20–40 min), identifying 55 unique volatiles. With the exception of hydrocarbons, DMU-SPME exhibited superior extraction efficiency and significantly better stability compared to both SAFE and SDE methods. Thus, DMU-SPME proves to be a promising alternative for the comprehensive extraction of volatile organic compounds.
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DMU-SPME、SAFE和SDE三种提取方法分析酱油中风味化合物的比较
将双模统一固相微萃取(DMU-SPME)与传统的溶剂基方法(包括溶剂辅助风味蒸发(SAFE)和同时蒸馏萃取(SDE))进行比较,探讨其作为一种替代方法的潜力。与SAFE和SDE相比,DMU-SPME是一种绿色的挥发性有机化合物提取技术。结果表明,DMU-SPME在提取中挥发性化合物(保留时间为20-40 min)方面明显优于SAFE和SDE,鉴定出55种独特的挥发物。除烃类外,与SAFE和SDE方法相比,DMU-SPME的萃取效率更高,稳定性也更好。因此,DMU-SPME被证明是一种有前途的挥发性有机化合物综合提取的替代方法。
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阿拉丁
anhydrous Na<sub>2</sub>SO<sub>4</sub>
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ethanol
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CH<sub>2</sub>Cl<sub>2</sub>
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15 standards for DMU-SPME condition optimization
来源期刊
Journal of Chromatography B
Journal of Chromatography B 医学-分析化学
CiteScore
5.60
自引率
3.30%
发文量
306
审稿时长
44 days
期刊介绍: The Journal of Chromatography B publishes papers on developments in separation science relevant to biology and biomedical research including both fundamental advances and applications. Analytical techniques which may be considered include the various facets of chromatography, electrophoresis and related methods, affinity and immunoaffinity-based methodologies, hyphenated and other multi-dimensional techniques, and microanalytical approaches. The journal also considers articles reporting developments in sample preparation, detection techniques including mass spectrometry, and data handling and analysis. Developments related to preparative separations for the isolation and purification of components of biological systems may be published, including chromatographic and electrophoretic methods, affinity separations, field flow fractionation and other preparative approaches. Applications to the analysis of biological systems and samples will be considered when the analytical science contains a significant element of novelty, e.g. a new approach to the separation of a compound, novel combination of analytical techniques, or significantly improved analytical performance.
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