Teresa Magalí Hoffmann , Mario Alberto Soria , Dante Javier Bueno , Yosef Daniel Huberman , María Ishida , Brian Sauders , Gregory Deiulio , Alyssa Wiedenmayer Dickey , José Luis Medina-Santana , Christian Vinueza-Burgos
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引用次数: 0
Abstract
The escalating issue of antimicrobial resistance in pathogens transmitted through the food chain poses a significant threat to public health worldwide. Salmonella ser. Enteritidis (SE) remains a predominant cause of foodborne illness. This research aims to elucidate the genetic and antimicrobial resistance profiles of SE strains isolated from Argentine poultry farms. This study used whole genome sequencing to assess the genetic diversity and resistance patterns of 20 SE strains collected from poultry farms in Entre Ríos, Argentina, between January 2020 and February 2021. Phenotypic assays were used to detect resistance to 50 antibiotics across 14 antimicrobial classes. Genes and mutations linked to antimicrobial resistance were pinpointed on both plasmids and chromosomes of the strains. All investigated strains exhibited resistance to nitrofurantoin, and a high percentage showed resistance or intermediate sensitivity to fluoroquinolones. Genomic analysis showed the presence of resistance genes associated with aminoglycosides, β-lactams, and quinolones. The study distinctly categorized the strains into those isolated from broilers and laying hens, highlighting a complex interplay of resistance and diversity within the poultry industry. This shows antimicrobial resistance within foodborne pathogens like SE and the importance of continuous surveillance systems to track the evolution and spread of resistance. The genomic insights from this study not only enhance understanding of the resistance mechanisms but also facilitate tracking the transmission dynamics of SE, which is crucial for developing targeted interventions.
期刊介绍：
Veterinary Microbiology is concerned with microbial (bacterial, fungal, viral) diseases of domesticated vertebrate animals (livestock, companion animals, fur-bearing animals, game, poultry, fish) that supply food, other useful products or companionship. In addition, Microbial diseases of wild animals living in captivity, or as members of the feral fauna will also be considered if the infections are of interest because of their interrelation with humans (zoonoses) and/or domestic animals. Studies of antimicrobial resistance are also included, provided that the results represent a substantial advance in knowledge. Authors are strongly encouraged to read - prior to submission - the Editorials (''Scope or cope'' and ''Scope or cope II'') published previously in the journal. The Editors reserve the right to suggest submission to another journal for those papers which they feel would be more appropriate for consideration by that journal.
Original research papers of high quality and novelty on aspects of control, host response, molecular biology, pathogenesis, prevention, and treatment of microbial diseases of animals are published. Papers dealing primarily with immunology, epidemiology, molecular biology and antiviral or microbial agents will only be considered if they demonstrate a clear impact on a disease. Papers focusing solely on diagnostic techniques (such as another PCR protocol or ELISA) will not be published - focus should be on a microorganism and not on a particular technique. Papers only reporting microbial sequences, transcriptomics data, or proteomics data will not be considered unless the results represent a substantial advance in knowledge.
Drug trial papers will be considered if they have general application or significance. Papers on the identification of microorganisms will also be considered, but detailed taxonomic studies do not fall within the scope of the journal. Case reports will not be published, unless they have general application or contain novel aspects. Papers of geographically limited interest, which repeat what had been established elsewhere will not be considered. The readership of the journal is global.
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