Triton CG-110 as an alternative for cell lysis in manufacturing of adeno-associated virus-based gene therapy

IF 2.8 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology Progress Pub Date : 2025-08-13 Epub Date: 2025-03-26 DOI:10.1002/btpr.70025

Yixuan Ming, Tianyi Zhou, Bin Lu, Yemaiza Ojeda-Lassalle, Pasquale Valerio, Lu Wang, Mi Jin

引用次数: 0

Abstract

Triton X-100 is one of the most widely used detergents for cell lysis in gene therapy product manufacturing. However, due to the aquatic toxicity of the degradation product, Triton X-100 is regulated by the European Chemical Agency as a substance of very high concern. In this study, we aim to identify eco-friendly detergent alternatives to replace Triton X-100. Tween 20, Tween 80, n-Dodecyl-β-D-maltoside (DDM), and Triton CG-110 were tested and compared with Triton X-100. Triton CG-110 demonstrated similar performance during the harvest and subsequent purification process for multiple AAV vectors at different scales. Therefore, Triton CG-110 represents an effective and environmentally safe detergent alternative to Triton X-100.

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Triton CG-110在制造腺相关病毒基因治疗中作为细胞裂解的替代品。

Triton X-100 是基因治疗产品生产中最广泛使用的细胞裂解洗涤剂之一。然而，由于降解产物具有水生毒性，Triton X-100 被欧洲化学品管理局列为高度关注物质。在这项研究中，我们旨在找出可替代 Triton X-100 的环保型洗涤剂。我们对吐温 20、吐温 80、正十二烷基-β-D-麦芽糖苷（DDM）和 Triton CG-110 进行了测试，并与 Triton X-100 进行了比较。对于不同规模的多种 AAV 载体，Triton CG-110 在收获和后续纯化过程中表现出相似的性能。因此，Triton CG-110 是替代 Triton X-100 的一种有效且环保安全的洗涤剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology Progress 工程技术-生物工程与应用微生物

CiteScore

6.50

自引率

3.40%

发文量

审稿时长

4 months

期刊介绍： Biotechnology Progress , an official, bimonthly publication of the American Institute of Chemical Engineers and its technological community, the Society for Biological Engineering, features peer-reviewed research articles, reviews, and descriptions of emerging techniques for the development and design of new processes, products, and devices for the biotechnology, biopharmaceutical and bioprocess industries. Widespread interest includes application of biological and engineering principles in fields such as applied cellular physiology and metabolic engineering, biocatalysis and bioreactor design, bioseparations and downstream processing, cell culture and tissue engineering, biosensors and process control, bioinformatics and systems biology, biomaterials and artificial organs, stem cell biology and genetics, and plant biology and food science. Manuscripts concerning the design of related processes, products, or devices are also encouraged. Four types of manuscripts are printed in the Journal: Research Papers, Topical or Review Papers, Letters to the Editor, and R & D Notes.