Fusarium oxysporum FO12 enhances plant performance by modulating the phytohormone profile and soil properties as a function of the inoculation method

IF 4.1 2区农林科学 Q1 AGRONOMY Plant and Soil Pub Date : 2025-03-27 DOI:10.1007/s11104-025-07380-z

Jesús Sevillano-Caño, Carlos Agustí-Brisach, José María García-Mina, Ángel María Zamarreño, Vidal Barrón, Antonio Rafael Sánchez-Rodríguez

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Abstract

Background and aims

Endophytic fungi such as the non-pathogenic strain Fusarium oxysporum FO12 have emerged as useful allies for plants as they could promote plant growth and protect from stresses. However, its performance under natural soil conditions and the effectiveness of different inoculation methods have not been evaluated before. The objectives of this study were to assess the ability of FO12 to enhance plant growth and nutrition in sunflower and wheat plants grown on soils differing in texture and organic matter content, pH, and nutrient availability, and to compare the effectiveness of two different inoculation methods (seed dressing, and soil treatment).

Methods

Firstly, we developed in vivo experiments to evaluate the ability of FO12 to increase nutrient availability. Secondly, we assessed the potential of FO12 for enhancing plant growth and nutrition in sunflower and wheat plants (in vivo) grown on four non-calcareous and calcareous soils with different nutrients availability (Fe, Zn and P); and the effectiveness of two different FO12 application methods, seed dressing (Sd) and soil treatment (St).

Results

FO12 modified the pH of the culture medium, and significantly increased the potential Fe availability by 202%, and Zn availability by 52% and P availability by 7% (but not significantly) compared to the non-inoculated control (in vitro). Both FO12 application methods boosted plant growth by increasing plant height as well as sunflower and wheat reproductive biomass by up to 27 − 31% and 28–34% in non-calcareous and calcareous soils (in vivo), respectively. FO12 additionally increased macro- and micronutrient uptake, especially with St. Finally, the fungus induced changes in the plant phytohormone profile, mainly with Sd, which consistently increased jasmonic acid (47%), jasmonyl isoleucine (511%) and 1-Aminocyclopropane-1-carboxylic acid (226%), and decreased indoleacetic acid (39%) across all four soils and in both crops, in comparison with the non-inoculated plants.

Conclusions

FO12 stimulates plant growth by altering soil properties and physiological plant processes, and its effects on plant nutrition and production depend on the plant species and soil type.

Graphical Abstract

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尖孢镰刀菌（Fusarium oxysporum FO12）通过调节植物激素分布和土壤特性来提高植株的生产性能

背景与目的内生真菌，如非致病性菌株镰刀菌FO12，已成为植物的有用盟友，因为它们可以促进植物生长和保护植物免受胁迫。但对其在自然土壤条件下的表现及不同接种方法的有效性尚无评价。本研究的目的是评估FO12在不同质地、有机质含量、pH值和养分有效性土壤上促进向日葵和小麦植株生长和营养的能力，并比较两种不同接种方法（修整种子和土壤处理）的效果。方法首先，通过体内实验来评价FO12提高营养物质利用率的能力。其次，我们评估了FO12在4种不同养分有效性（铁、锌、磷）的非钙质和钙质土壤上促进向日葵和小麦植株生长和营养的潜力；两种不同施肥方式（Sd）和土壤处理（St）的效果。结果与未接种对照相比，fo12对培养液的pH值进行了修饰，使铁、锌、磷的潜在有效度分别提高了202%、52%和7%（但不显著）。在非钙质土壤和钙质土壤中，施用FO12可使向日葵和小麦的株高和繁殖生物量分别提高27 - 31%和28-34%，促进植物生长。最后，真菌诱导植物激素分布发生变化，主要是Sd，与未接种植株相比，4种土壤和两种作物的茉莉酸（47%）、茉莉异黄酮（511%）和1-氨基环丙烯-1-羧酸（226%）持续增加，吲哚乙酸（39%）持续减少。结论fo12通过改变土壤性质和植物生理过程促进植物生长，其对植物营养和生产的影响与植物种类和土壤类型有关。图形抽象

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来源期刊

Plant and Soil 农林科学-农艺学

CiteScore

8.20

自引率

8.20%

发文量

543

审稿时长

2.5 months

期刊介绍： Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.