Facile fabrication of biocompatible polymeric microspheres using an AIE-active cellulose derivative for cell imaging

IF 4.2 3区工程技术 Q2 CHEMISTRY, APPLIED Dyes and Pigments Pub Date : 2025-08-01 Epub Date: 2025-03-22 DOI:10.1016/j.dyepig.2025.112779

Chunping Ma , Jiyin He , Gaoyi Xie , Yuanyuan Han , Fengyuan Lin , Qiankun Guo , Dongdong Cao , Jinpeng Mo , Haijun Ma , Haibo Xie , Zhenguo Chi , Yongcan Jin

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Abstract

Fluorescent polymeric micromaterials have attracted abiding interest in biomedical applications due to their merits of visualization, fast response, and high sensitivity. Herein, we report a facile strategy for the fabrication of biocompatible polymeric microspheres using an aggregation-induced emission (AIE) active cellulose derivative TPE-CDHPM, which was synthesized through an esterification reaction of microcrystalline cellulose (MCC) and a subsequent Biginelli reaction. The chemical structures of TPE-CDHPM were determined by FT-IR and ¹H NMR spectroscopies, displaying a high degree of substitution of TPE (DS_TPE = 0.73). TPE-CDHPM showed extremely weak fluorescence in the mono-dispersed state while emitting intensively at aggregated state. When dispersed in water, TPE-CDHPM could aggregate into microspheres with a size range of 40–200 nm. The TPE-CDHPM microspheres exhibited an ultralow critical micelle concentration (CMC) of 0.006 mg mL⁻¹, good photoluminescence stability, negligible cytotoxicity, and fascinating biocompatibility, allowing its application in cell imaging. This work offers a direction for effectively constructing biocompatible fluorescent polymeric micromaterials for biomedical applications.

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利用aie活性纤维素衍生物制备生物相容性聚合物微球用于细胞成像

荧光高分子微材料具有可视化、快速响应和高灵敏度等优点，在生物医学领域的应用受到了广泛的关注。本文报道了一种利用聚集诱导发射（AIE）活性纤维素衍生物TPE-CDHPM制备生物相容性聚合物微球的简单策略，该衍生物是通过微晶纤维素（MCC）的酯化反应和随后的Biginelli反应合成的。通过FT-IR和1H NMR对TPE- cdhpm的化学结构进行了表征，发现TPE取代度较高（DSTPE = 0.73）。TPE-CDHPM在单分散状态下荧光极弱，在聚集状态下荧光强烈。当TPE-CDHPM分散在水中时，可以聚集成40-200 nm大小的微球。TPE-CDHPM微球具有0.006 mg mL−1的超低临界胶束浓度（CMC）、良好的光致发光稳定性、可忽略的细胞毒性和良好的生物相容性，可用于细胞成像。本研究为有效构建生物相容性荧光高分子微材料提供了方向。

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来源期刊

Dyes and Pigments 工程技术-材料科学：纺织

CiteScore

8.20

自引率

13.30%

发文量

933

审稿时长

33 days

期刊介绍： Dyes and Pigments covers the scientific and technical aspects of the chemistry and physics of dyes, pigments and their intermediates. Emphasis is placed on the properties of the colouring matters themselves rather than on their applications or the system in which they may be applied. Thus the journal accepts research and review papers on the synthesis of dyes, pigments and intermediates, their physical or chemical properties, e.g. spectroscopic, surface, solution or solid state characteristics, the physical aspects of their preparation, e.g. precipitation, nucleation and growth, crystal formation, liquid crystalline characteristics, their photochemical, ecological or biological properties and the relationship between colour and chemical constitution. However, papers are considered which deal with the more fundamental aspects of colourant application and of the interactions of colourants with substrates or media. The journal will interest a wide variety of workers in a range of disciplines whose work involves dyes, pigments and their intermediates, and provides a platform for investigators with common interests but diverse fields of activity such as cosmetics, reprographics, dye and pigment synthesis, medical research, polymers, etc.