Droplet-Enhanced Chemiluminescence Strategy for Enrofloxacin Detection Based on Porous Carbon Z-8 Encapsulated Luminol

Abstract

A droplet-enhanced chemiluminescence (CL) assay for enrofloxacin was fabricated. After preparation ZIF-8 carbonization, porous carbon material Z-800 was obtained and aptamer and cDNA were immobilizated onto the surface of Z-800. Luminol was encapsulated into Z-800 with the help of aptamer and cDNA. In the presence of enrofloxacin, aptamer accurately recognizes it, enrofloxacin specifically binds to aptamer, and the switch that encapsulateed luminol is turned on, causing luminol to be released into the supernatant. The supernatant reacts with H₂O₂ to produce a strong CL signal. The immiscible organic droplets, benzyl benzoate (Bnbzo), greatly enhances the CL signal. This assay shows a linear relationship between enhanced CL signals and enrofloxacin concentrations in the range from 0.1 to 500.0 nM with LOD of 30 pM (S/N = 3). The sample measurement was performed with the recoveries of 92.00% to 95.63% and relative standard deviation of 3.9%–5.2%. It proves that droplet-enhanced CL is a promising method for enhancing CL signals in real sample and trace analysis.