Lack of significant effect of gut microbiota on weight gain in newly emerged worker honeybee.

Abstract

The western honeybee, Apis mellifera, harbours a simple and distinct microbiota that has been linked to various positive outcomes for the host. Among these cited benefits is improved weight gain for bees that have been inoculated with their native microbes. This result has been challenged by recent studies which investigated the impact of the gut microbiota on behavioural maturation and associated physiological changes and revealed no effect of the gut microbiota on weight gain. Therefore, we re-examined the role of the microbiota in weight gain by comparing microbiota-deprived bees with those inoculated with gut homogenate or defined communities composed of isolates representing the major bacterial taxa inhabiting the bee gut. We observed no differences in weight gain of adult bees or of their gut tissues across these groups. Further analysis based on nurse/forager cuticular hydrocarbon status and bacterial composition also revealed no significant changes. These results suggest the need for more nuanced investigations aimed at exploring factors such as the conditions in the hive of origin, including larval nutrition.