Identification of the WRKY gene family in Bergenia purpurascens and functional analysis of BpWRKY13 under cold stress

IF 5.7 2区生物学 Q1 PLANT SCIENCES Plant Physiology and Biochemistry Pub Date : 2025-03-25 DOI:10.1016/j.plaphy.2025.109832

Jingyu Chen, Hongyan Zeng, Feiyang Yan, Zongxiang Jiang, Jie Chen, Wenqing Wang, Qiankun Zhu

{"title":"Identification of the WRKY gene family in Bergenia purpurascens and functional analysis of BpWRKY13 under cold stress","authors":"Jingyu Chen, Hongyan Zeng, Feiyang Yan, Zongxiang Jiang, Jie Chen, Wenqing Wang, Qiankun Zhu","doi":"10.1016/j.plaphy.2025.109832","DOIUrl":null,"url":null,"abstract":"<div><div>Bergenia purpurascens, a medicinal alpine plant, exhibits remarkable stress resilience. WRKY transcription factors are central regulators of plant stress responses, yet their family in B. purpurascens remains uncharacterized. Here, we identified 57 BpWRKY genes from B. purpurascens transcriptome data. Expression analysis revealed 11 BpWRKY genes differentially expressed under cold stress, with BpWRKY13 showing the strongest induction. To investigate its function, we overexpressed BpWRKY13 in Arabidopsis thaliana. Transgenic plants displayed significantly enhanced cold tolerance, evidenced by reduced leaf damage, increased survival, and elevated accumulation of proline and soluble proteins. Furthermore, transgenic plants exhibited increased activity of antioxidant enzymes and upregulation of cold-responsive genes. These findings indicate that BpWRKY13 confers cold tolerance by promoting osmoprotection and activating antioxidant defense mechanisms. This study provides a crucial foundation for understanding the BpWRKY gene family and highlights BpWRKY13 as a key regulator of cold resistance in B. purpurascens.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109832"},"PeriodicalIF":5.7000,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology and Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0981942825003602","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Bergenia purpurascens, a medicinal alpine plant, exhibits remarkable stress resilience. WRKY transcription factors are central regulators of plant stress responses, yet their family in B. purpurascens remains uncharacterized. Here, we identified 57 BpWRKY genes from B. purpurascens transcriptome data. Expression analysis revealed 11 BpWRKY genes differentially expressed under cold stress, with BpWRKY13 showing the strongest induction. To investigate its function, we overexpressed BpWRKY13 in Arabidopsis thaliana. Transgenic plants displayed significantly enhanced cold tolerance, evidenced by reduced leaf damage, increased survival, and elevated accumulation of proline and soluble proteins. Furthermore, transgenic plants exhibited increased activity of antioxidant enzymes and upregulation of cold-responsive genes. These findings indicate that BpWRKY13 confers cold tolerance by promoting osmoprotection and activating antioxidant defense mechanisms. This study provides a crucial foundation for understanding the BpWRKY gene family and highlights BpWRKY13 as a key regulator of cold resistance in B. purpurascens.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

紫癜性卑尔根菌WRKY基因家族的鉴定及BpWRKY13在冷胁迫下的功能分析

紫荆是一种高山药用植物，具有显著的抗逆性。WRKY转录因子是植物胁迫反应的中心调控因子，但其在紫癜双歧杆菌中的家族特征尚不明确。在此，我们从紫癜双球菌转录组数据中鉴定出57个BpWRKY基因。表达分析显示，11个BpWRKY基因在冷胁迫下差异表达，其中BpWRKY13基因的诱导作用最强。为了研究其功能，我们在拟南芥中过表达BpWRKY13。转基因植株表现出显著增强的耐寒性，叶片损伤减少，存活率提高，脯氨酸和可溶性蛋白积累增加。此外，转基因植株抗氧化酶活性增加，冷响应基因表达上调。这些发现表明BpWRKY13通过促进渗透保护和激活抗氧化防御机制来赋予耐寒性。该研究为了解BpWRKY基因家族提供了重要的基础，并强调了BpWRKY13在紫癜双球菌抗寒性中的关键调控作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.