Yaxuan Jiang , Pei Lei , Jianxin Li , Ximei Ji , Yong Zhao , Xiyang Zhao , Fanjuan Meng
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引用次数: 0
Abstract
In early spring, bleeding is a common occurrence in A. arguta when local temperatures reach 8 °C–10 °C. In this study, changes in the composition of bleeding sap and effect of bleeding on A. arguta were determined by analyzing the variation in the composition of bleeding sap from different periods. Accordingly, the bleeding sap was analyzed by gas chromatography-mass spectrometry (GC-MS), and the related physiological indexes in the stem were measured. The results revealed that in annual early spring, bleeding seriously affected A. arguta. In addition, annual branches had a stronger capacity for scavenging free radicals than biennial or barren branches. The metabolomics analysis identified a total of 153 differential metabolites, mainly involved in glycolysis and the tricarboxylic acid cycle. Bleeding decreased the number and length of new shoots and basal stems, as well as the size of the phloem, phloem fibers, and xylem. In contrast, the proline, soluble protein, soluble sugar, and malondialdehyde contents, as well as the activities of superoxide dismutase, catalase, and peroxidase, increased in treated stems compared with untreated controls. This study was the first to analyze the metabolomic profile of bleeding sap in A. arguta and investigate the effects of bleeding on its growth and development. The results will provide a theoretical basis for explaining the physiological changes occurring in stems after bleeding and lay a foundation for further research on understanding the underlying mechanism and repairing bleeding.
期刊介绍：
Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement.
Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB.
Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.
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