Genomic analysis and metabolic pathway mining of Phallus cremeo-ochraceus

Abstract

Phallus cremeo-ochraceus is a nutritious edible mushroom. In this study, after tissue isolation, 33 strains were obtained, among which one strain PC7 with rapid mycelial growth and stable passage was obtained. Here, after quality control and assembly, a sequence of 410,647,36 bp was obtained, with 20,218 contigs. 23,184 genes were predicted, 8,092 were repetitive sequences, and 165 were non-coding RNA (ncRNA), which was obtained by the Illumina platform. NCBI Blast+ was used to compare the protein sequences of the genes with several databases, such as NR, KOG, GO, KEGG, CAzy, etc. The NR database annotated 18,159 genes, KOG annotated 6752 genes, GO annotated 5872 genes, and KEGG annotated 3820 genes. Exploration of the terpenoid synthesis pathway of strain PC7 by KEGG, the results revealed that the genome of PC7 has a more complete metabolic pathway regarding terpenoid synthesis. The analysis of amino acid metabolic pathways shows that Phallus cremeo-ochraceus contains 18 genes related to amino acid metabolic pathways. Combined with carbohydrate enzyme annotation and KEGG annotation, the genes related to carbon source, nitrogen source, sulfur source and growth factor of Phallus cremeo-ochraceus were analyzed, which enriched the related genomic research of Phallus cremeo-ochraceus.