Studies on adsorption and synergistic biological effects induced by microplastic particles and the Platanus pollen allergenic protein 3(Pla a3)

Abstract

Microplastics (MPs) are pervasive as emerging pollutants in ambient particles and may pose a potential threat to human health through respiratory exposure. Especially, impact of climate change has led to an extended blooming period for many plants, resulting in elevated pollen levels in the air, and leading to a continuous increase in the number of individuals suffering from allergenic diseases. However, the interactions between the MPs and allergenic proteins, remain largely unexplored. In this study, we investigated cellular toxicity of the MPs and Platanus pollen allergenic protein (Pla a3) based on the characterization of two typical microplastics (polystyrene, PS and polyethylene, PE). Our results indicated that UV irradiation could make surface alterations of the MPs, including breakage, particle size reduction, and an increase in surface oxygen-containing functional groups. These changes significantly enhanced the adsorption of the Pla a 3 protein. The 'protein coronas' formed by the MPs and the Pla a3 caused more damage to the A549 cells than Pla a3 alone. Reactive oxygen species (ROS) generation and elevated superoxide dismutase (SOD) levels increased significantly after the A549 cells were exposure to the protein coronas. This excessive oxidative stress led to significant inflammation and cytokine production increase, with IL-1β, IL-4, IFN-γ, and TNF-α levels rising by 1.84 ± 0.01, 2.37 ± 0.04, 1.94 ± 0.09, and 2.19 ± 0.05-fold times respectively compared to that of the Pla a 3 exposure alone. This study provided a fundamental data for further research for the allergenicity induced by the pollen proteins.