Evaluation of the effect of platelet-derived growth factor-BB on the biological activity of human mandibular bone marrow-derived mesenchymal stem cells
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Abstract
Objective
This study aimed to investigate the effects of platelet-derived growth factor-BB (PDGF-BB) on the biological activities of human mandibular bone marrow-derived mesenchymal stem cells (MBMSCs).
Design
PDGF-BB (20 ng/mL) was used to treat MBMSCs, and its effects on their proliferation, osteogenic differentiation, and migration were evaluated. Cell proliferation was evaluated using a WST-1 assay. Osteogenic differentiation was evaluated by measuring the mineralization potential and alkaline phosphatase activity. Cell migration was evaluated using wound healing and Transwell chamber assays. Cytoskeletal reorganization and adhesion dynamics were evaluated using immunofluorescence staining. Changes in intracellular signaling in MBMSCs induced by PDGF-BB stimulation were evaluated using western blotting. Furthermore, we investigated Girdin signaling as the molecular mechanisms underlying the regulation of PDGF-BB-induced cell migration.
Results
PDGF-BB treatment did not affect the proliferation or osteogenic differentiation of MBMSCs. PDGF-BB promoted the migration of MBMSCs. PDGF-BB treatment enhanced F-actin filament formation and paxillin localization at the leading edge of cells. PDGF-BB treatment activated Akt signaling in MBMSCs, and the inhibition of Akt signaling effectively suppressed PDGF-BB-induced Akt activation and migration. PDGF-BB promoted the phosphorylation of Girdin in MBMSCs, and the inhibition of Akt signaling attenuated PDGF-BB-induced Girdin activation.
Conclusion
This study demonstrated that PDGF-BB strongly induces the migration of MBMSCs without affecting their proliferation or osteogenic differentiation. Furthermore, PDGF-BB-induced migration of MBMSCs may be mediated through the Akt/Girdin signaling pathway. These findings provide important insight into the molecular mechanisms underlying PDGF-BB-induced periodontal tissue regeneration.
期刊介绍：
Archives of Oral Biology is an international journal which aims to publish papers of the highest scientific quality in the oral and craniofacial sciences. The journal is particularly interested in research which advances knowledge in the mechanisms of craniofacial development and disease, including:
Cell and molecular biology
Molecular genetics
Immunology
Pathogenesis
Cellular microbiology
Embryology
Syndromology
Forensic dentistry
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