Low-speed centrifugation based isolation and self-priming mediated chain extension based fluorescent quantification of Pseudomonas aeruginosa

Abstract

Infections acquired at home and hospital are rather prevalent, and the incidence of these infections has been on the rise in recent years due to the growing elderly population. Infections caused by Pseudomonas aeruginosa (P. aeruginosa) pose a significant risk to human health and are prevalent among patients in hospitals and nursing homes. Consequently, it is imperative to devise an innovative and fluorescent method for analyzing P. aeruginosa to facilitate the early identification of home-acquired pneumonia. However, it is difficult to isolate and simultaneously quantify P. aeruginosa using most of the currently available methods. We present a novel platform that combines aptamer recognition-based aggregation of target bacteria with self-priming induced chain extension for signal amplification. This approach facilitates low-speed centrifugation-based isolation and simultaneous quantification of P. aeruginosa. The chain displacement procedure is incorporated for signal amplification, providing the approach with a broad detection range of six orders of magnitude and a low detection limit of 2.4 cfu/mL. In addition to its exceptional sensitivity, the method demonstrates commendable selectivity for the detection of P. aeruginosa, rendering it a viable instrument for identifying home-acquired pneumonia caused by P. aeruginosa and facilitating the early management of P. aeruginosa infections in the emergency department.