Immune restoration mechanism analysis of Akkermansia PROBIO in mice treated with cyclophosphamide through Re-balancing microbiota composition

Abstract

Currently, a variety of viruses are assaulting people's bodies, making it especially crucial to enhance immunity. Akkermansia, a potential next-generation probiotic, has been reported to keep human health. Akkermansia PROBIO is a safety stain obtained for improving gut flora health. This study aimed to investigate the protective effects of Akkermansia PROBIO against immunosuppression induced by cyclophosphamide in mice. The findings indicated that Akkermansia PROBIO has the potential to alleviate immune damage caused by cyclophosphamide by restoring cytokine levels and regulating the expression of Claudins-1, ZO-1, and Occludin. Importantly, Akkermansia PROBIO would keep intestinal health via re-balancing microbiota composition including increasing Lactobacillus levels and reducing Proteobacteria levels. Simultaneously, a more in-depth investigation was conducted to reveal important associations between various microbiomes and the levels of short-chain fatty acids, aiming to examine the immune restoration process linked to Akkermansia PROBIO. The findings indicate that Akkermansia PROBIO is vital in influencing the gut microbiome, thus aiding in broader immune defense. This research offers potential probiotic candidates for enhancing human immunity.