LncRNA SPINT1-AS1 enhances the Warburg effect and promotes the progression of osteosarcoma via the miR-135b-5p/PGAM1 axis.

IF 6 2区医学 Q1 ONCOLOGY Cancer Cell International Pub Date : 2025-04-01 DOI:10.1186/s12935-025-03761-7

Jinyou Wang, Ning Zhang, Yan Wang, Fanghong Chen, Lei Wang, Kailun Wang, Xiao Yao, Xiaohui Pan

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Abstract

Osteosarcoma (OS) is a malignant bone tumor that originates from interstitial tissues and affects the health of children and adolescents. Long noncoding RNAs (lncRNAs) are an intriguing category of widely distributed endogenous RNAs involved in OS progression, many of which remain functionally uncharacterized. Herein, we observed an increased expression of lncRNA SPINT1-AS1 in OS tissues and cell lines. Further analysis confirmed that SPINT1-AS1 promotes the proliferation and metastasis of OS cells. Moreover, miR-135b-5p was identified as a downstream target of SPINT1-AS1. Through bioinformatics analysis, PGAM1 mRNA was validated as a target of miR-135b-5p via RIP and luciferase reporter assays. SPINT1-AS1 could enhance OS cell proliferation and metastasis by promoting aerobic glycolysis, acting as a ceRNA by binding to miR-135b-5p, thereby increasing PGAM1 expression. Taken together, these results indicate that SPINT1-AS1 functions as a tumor promoter and regulates OS cell progression through the miR-135b-5p/PGAM1 axis.

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LncRNA SPINT1-AS1通过miR-135b-5p/PGAM1轴增强Warburg效应并促进骨肉瘤的进展。

骨肉瘤（Osteosarcoma， OS）是一种起源于间质组织的恶性骨肿瘤，影响儿童和青少年的健康。长链非编码rna （lncRNAs）是广泛分布的内源性rna中一个有趣的类别，参与了OS的进展，其中许多仍未被功能表征。在此，我们观察到lncRNA SPINT1-AS1在OS组织和细胞系中的表达增加。进一步分析证实SPINT1-AS1促进OS细胞的增殖和转移。此外，miR-135b-5p被鉴定为SPINT1-AS1的下游靶点。通过生物信息学分析，通过RIP和荧光素酶报告基因检测验证PGAM1 mRNA是miR-135b-5p的靶标。SPINT1-AS1可以通过促进有氧糖酵解促进OS细胞增殖和转移，通过结合miR-135b-5p作为ceRNA，从而增加PGAM1的表达。综上所述，这些结果表明SPINT1-AS1作为肿瘤启动子并通过miR-135b-5p/PGAM1轴调节OS细胞进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cancer Cell International ONCOLOGY-

CiteScore

10.90

自引率

1.70%

发文量

360

审稿时长

1 months

期刊介绍： Cancer Cell International publishes articles on all aspects of cancer cell biology, originating largely from, but not limited to, work using cell culture techniques. The journal focuses on novel cancer studies reporting data from biological experiments performed on cells grown in vitro, in two- or three-dimensional systems, and/or in vivo (animal experiments). These types of experiments have provided crucial data in many fields, from cell proliferation and transformation, to epithelial-mesenchymal interaction, to apoptosis, and host immune response to tumors. Cancer Cell International also considers articles that focus on novel technologies or novel pathways in molecular analysis and on epidemiological studies that may affect patient care, as well as articles reporting translational cancer research studies where in vitro discoveries are bridged to the clinic. As such, the journal is interested in laboratory and animal studies reporting on novel biomarkers of tumor progression and response to therapy and on their applicability to human cancers.