Phosphorylation of the strawberry MADS-box CMB1 regulates ripening via the catabolism of abscisic acid

IF 8.1 1区 生物学 Q1 PLANT SCIENCES New Phytologist Pub Date : 2025-04-02 DOI:10.1111/nph.70065
Haoran Jia, Yanna Shi, Zhengrong Dai, Yunfan Sun, Xiu Shu, Baijun Li, Rongrong Wu, Shouzheng Lv, Jiahan Shou, Xiaofang Yang, Guihua Jiang, Yuchao Zhang, Andrew C. Allan, Kunsong Chen
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草莓 MADS-box CMB1 的磷酸化通过脱落酸的分解来调节成熟。
肉质水果成熟的研究依赖于测量转录变化的技术。成熟是如何与翻译后修饰(如蛋白质磷酸化)联系在一起的,目前研究较少。本研究对MADS-box SEPALLATA 4 (SEP4)亚家族转录因子FaCMB1进行了表征,该亚家族转录因子FaCMB1是草莓成熟的关键负调控因子,其转录量和蛋白丰度随着果实发育而逐渐降低,并受到脱落酸(ABA)的抑制。瞬时RNAi或过表达FaCMB1显著改变果实成熟过程,影响内源ABA含量和成熟相关品质。转录组测序(RNA-seq)分析表明,操纵FaCMB1表达水平会影响FaASR的转录(ABA-、胁迫-、成熟诱导),而FaCMB1可以通过直接结合FaASR启动子抑制FaASR基因的表达。此外,FaASR还抑制了参与ABA分解代谢的关键ABA 8′-羟化酶FaCYP707A4的转录活性。我们发现FaCMB1可以被FaSTPK激酶磷酸化,Phos-tag实验表明,FaCMB1的磷酸化水平在果实成熟过程中增加。FaCMB1的磷酸化影响了FaCMB1与FaASR启动子的结合能力,减轻了其转录抑制作用。总之,我们阐明了一个涉及FaCMB1-FaASR-FaCYP707A4-ABA的反馈调控路径。在果实成熟过程中,ABA含量的增加导致FaCMB1转录物和蛋白水平的降低,并结合磷酸化水平的增加,共同破坏了FaCMB1对FaASR的转录抑制。同时,FaASR转录水平的升高进一步抑制FaCYP707A4的表达水平,导致ABA积累和果实成熟。
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来源期刊
New Phytologist
New Phytologist 生物-植物科学
自引率
5.30%
发文量
728
期刊介绍: New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.
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