Sample Preparation of Polar Metabolites in Biological Samples: Methodologies and Technological Insights

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL Trends in Analytical Chemistry Pub Date : 2025-03-26 DOI:10.1016/j.trac.2025.118244

Xue-Man Dong , Frederik André Hansen , Pu Wu , Yelu Wu , Sile He , Weiyang Tang

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Abstract

Polar metabolites in biological samples are vital for various biochemical processes and serve as key indicators in numerous clinical and experimental investigations. However, the inherent complexity of biological matrices and the strong polarity of metabolites present significant challenges in sample preparation, resulting in inefficient extraction and matrix effects. Therefore, continuous advances in pre-treatment methodologies are crucial for improving sensitivity, reproducibility, and overall analytical performance. This review provides a comprehensive overview of advancements in analytical preparation techniques for polar metabolites in biological samples since 2020. It examines the strengths and limitations of these methods, focusing on their applications in extracting and analyzing diverse classes of polar metabolites. By exploring the latest trends and developments in sample processing, this review aims to assist researchers in selecting appropriate techniques for efficient and reliable metabolite analysis, ultimately improving the accuracy and depth of biochemical research.

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生物样品中极性代谢物的样品制备：方法和技术见解

生物样品中的极性代谢物对各种生化过程至关重要，并在许多临床和实验研究中作为关键指标。然而，生物基质固有的复杂性和代谢物的强极性给样品制备带来了重大挑战，导致提取效率低下和基质效应。因此，预处理方法的不断进步对于提高灵敏度、重现性和整体分析性能至关重要。本文综述了自2020年以来生物样品中极性代谢物分析制备技术的进展。它考察了这些方法的优势和局限性，重点介绍了它们在提取和分析不同类别的极性代谢物方面的应用。通过探讨样品处理的最新趋势和发展，本文旨在帮助研究人员选择合适的技术进行高效可靠的代谢物分析，最终提高生化研究的准确性和深度。

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.