{"title":"Comprehensive characterization of volatile terpenoids and terpene synthases in Lanxangia tsaoko.","authors":"Shanshan Chen, Mofan Zhang, Shuo Ding, Zhichao Xu, Sifan Wang, Xiangxiao Meng, Shilin Chen, Ranran Gao, Wei Sun","doi":"10.1186/s43897-024-00140-0","DOIUrl":null,"url":null,"abstract":"<p><p>Lanxangia tsaoko is widely utilized in human cuisine as a popular flavoring agent due to its distinctive aroma. It also has a long history of use in traditional Chinese medicine. The edible and medicinal properties of L. tsaoko are primarily attributed to its diverse array of volatile metabolites. Previous research has mainly focused on classifying the constituents and their pharmacological activities in L. tsaoko, leaving gaps in comprehensive identification and elucidation of the biosynthetic mechanisms of these metabolites. In this study, we employed a multi-omics approach and functional characterization to investigate the biosynthesis of volatile terpenoids in L. tsaoko. The results demonstrated that terpenoids constituted the highest proportion of volatile compounds in L. tsaoko. Additionally, 42 terpene synthase (TPS) coding genes were identified through genome-wide analysis. Functional characterization revealed that eight LtTPSs effectively catalyzed geranyl pyrophosphate to produce monoterpenoids, while four LtTPSs converted farnesyl pyrophosphate to generate sesquiterpenoids. Genome-wide and single-gene duplication events contributed to functional diversification among LtTPSs with high identity, promoting the diversity of terpenoids. These findings provide a foundation for understanding the biosynthesis of volatile terpenoids in L. tsaoko, enhance the current knowledge of TPS, and contribute to the broader understanding of the biochemical diversity of terpenoids in plants.</p>","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"5 1","pages":"20"},"PeriodicalIF":8.1000,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11966916/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Horticulture","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s43897-024-00140-0","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"HORTICULTURE","Score":null,"Total":0}
引用次数: 0
Abstract
Lanxangia tsaoko is widely utilized in human cuisine as a popular flavoring agent due to its distinctive aroma. It also has a long history of use in traditional Chinese medicine. The edible and medicinal properties of L. tsaoko are primarily attributed to its diverse array of volatile metabolites. Previous research has mainly focused on classifying the constituents and their pharmacological activities in L. tsaoko, leaving gaps in comprehensive identification and elucidation of the biosynthetic mechanisms of these metabolites. In this study, we employed a multi-omics approach and functional characterization to investigate the biosynthesis of volatile terpenoids in L. tsaoko. The results demonstrated that terpenoids constituted the highest proportion of volatile compounds in L. tsaoko. Additionally, 42 terpene synthase (TPS) coding genes were identified through genome-wide analysis. Functional characterization revealed that eight LtTPSs effectively catalyzed geranyl pyrophosphate to produce monoterpenoids, while four LtTPSs converted farnesyl pyrophosphate to generate sesquiterpenoids. Genome-wide and single-gene duplication events contributed to functional diversification among LtTPSs with high identity, promoting the diversity of terpenoids. These findings provide a foundation for understanding the biosynthesis of volatile terpenoids in L. tsaoko, enhance the current knowledge of TPS, and contribute to the broader understanding of the biochemical diversity of terpenoids in plants.
期刊介绍：
Aims
Molecular Horticulture aims to publish research and review articles that significantly advance our knowledge in understanding how the horticultural crops or their parts operate mechanistically. Articles should have profound impacts not only in terms of high citation number or the like, but more importantly on the direction of the horticultural research field.
Scope
Molecular Horticulture publishes original Research Articles, Letters, and Reviews on novel discoveries on the following, but not limited to, aspects of horticultural plants (including medicinal plants):
▪ Developmental and evolutionary biology
▪ Physiology, biochemistry and cell biology
▪ Plant-microbe and plant-environment interactions
▪ Genetics and epigenetics
▪ Molecular breeding and biotechnology
▪ Secondary metabolism and synthetic biology
▪ Multi-omics dealing with data sets of genome, transcriptome, proteome, metabolome, epigenome and/or microbiome.
The journal also welcomes research articles using model plants that reveal mechanisms and/or principles readily applicable to horticultural plants, translational research articles involving application of basic knowledge (including those of model plants) to the horticultural crops, novel Methods and Resources of broad interest.
In addition, the journal publishes Editorial, News and View, and Commentary and Perspective on current, significant events and topics in global horticultural fields with international interests.
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