Calcium Imaging in Vivo: How to Correctly Select and Apply Fiber Optic Photometric Indicators.

IF 2.8 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Organogenesis Pub Date : 2025-12-01 Epub Date: 2025-04-05 DOI:10.1080/15476278.2025.2489667

Lanxia Wu, Wenxuan Sun, Linjie Huang, Lin Sun, Jinhua Dou, Guohua Lu

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Abstract

Fiber-photometric is a novel optogenetic method for recording neural activity in vivo, which allows the use of calcium indicators to observe and study the relationship between neural activity and behavior in free-ranging animals. Calcium indicators also convert changes in calcium concentration in cells or tissues into recordable fluorescent signals, which can then be observed using the system of fiber-photometric. To date, there is a paucity of relevant literature on the proper selection and application of fiber-photometric indicators. Therefore, this paper will detail how to correctly select and apply fiber-photometer indicators in four sections: the basic principle of optical fiber photometry, the selection of calcium fluorescent probes and viral vector systems, and the measurement of specific expression of fluorescent proteins in specific tissues. Therefore, the correct use of suitable fiber optic recording indicators will greatly assist researchers in exploring the link between neuronal activity and neuropsychiatric disorders.

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体内钙成像：如何正确选择和应用光纤光度指标。

纤维光度法是一种记录体内神经活动的新型光遗传学方法，它允许使用钙指示剂来观察和研究自由放养动物神经活动与行为之间的关系。钙指示剂还可以将细胞或组织中钙浓度的变化转化为可记录的荧光信号，然后使用纤维光度法系统对其进行观察。迄今为止，关于纤维光度指示剂的正确选择和应用的相关文献很少。因此，本文将从光纤光度法的基本原理、钙荧光探针和病毒载体系统的选择、荧光蛋白在特定组织中的特异性表达的测量四个部分详细介绍如何正确选择和应用光纤光度计指标。因此，正确使用合适的光纤记录指标将极大地帮助研究人员探索神经元活动与神经精神疾病之间的联系。

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来源期刊

Organogenesis BIOCHEMISTRY & MOLECULAR BIOLOGY-DEVELOPMENTAL BIOLOGY

CiteScore

4.10

自引率

4.30%

发文量

审稿时长

>12 weeks

期刊介绍： Organogenesis is a peer-reviewed journal, available in print and online, that publishes significant advances on all aspects of organ development. The journal covers organogenesis in all multi-cellular organisms and also includes research into tissue engineering, artificial organs and organ substitutes. The overriding criteria for publication in Organogenesis are originality, scientific merit and general interest. The audience of the journal consists primarily of researchers and advanced students of anatomy, developmental biology and tissue engineering. The emphasis of the journal is on experimental papers (full-length and brief communications), but it will also publish reviews, hypotheses and commentaries. The Editors encourage the submission of addenda, which are essentially auto-commentaries on significant research recently published elsewhere with additional insights, new interpretations or speculations on a relevant topic. If you have interesting data or an original hypothesis about organ development or artificial organs, please send a pre-submission inquiry to the Editor-in-Chief. You will normally receive a reply within days. All manuscripts will be subjected to peer review, and accepted manuscripts will be posted to the electronic site of the journal immediately and will appear in print at the earliest opportunity thereafter.