Synthesis and Characterization of Free Radical Scavenging Dendrimer Nanogels via Cross-Linking Reaction-Enabled Flash Nanoprecipitation

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Biomacromolecules Pub Date : 2025-05-12 DOI:10.1021/acs.biomac.5c00050

Lin Qi , Da Huang , Huari Kou , Anna Chernatynskaya , Nuran Ercal , Hu Yang

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Abstract

This work reports the development and evaluation of dendrimer-based nanogels based on polyamidoamine (PAMAM) dendrimer generation 5, engineered to act as a carrier with reactive oxygen species (ROS)-scavenging capabilities. We developed a cross-linking reaction-enabled flash nanoprecipitation method in which the cross-linking reaction occurs during the flash nanoprecipitation process to form a cross-linked nanostructure. Using this approach, an N-hydroxysuccinimide (NHS)-functionalized ROS-responsive thioketal cross-linker (TK-NHS) was synthesized and utilized to cross-link DAB-core PAMAM dendrimer G5, resulting in the formation of G5-TK nanogels. The resulting nanogels were characterized using dynamic light scattering and transmission electron microscopy, and their cytocompatibility, irritancy, cellular uptake, and ROS scavenging activity were assessed. We confirmed the ROS scavenging capability of these nanogels and observed favorable safety profiles. The G5-TK nanogels can be further developed as carriers for therapeutic delivery applications to treat oxidative stress-related pathological conditions.

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通过交联反应激活的纳米闪蒸法合成和表征清除自由基的树状聚合物纳米凝胶。

本研究报告了基于聚胺胺（PAMAM）第5代树状大分子的树状大分子纳米凝胶的开发和评价，该纳米凝胶被设计为具有活性氧（ROS）清除能力的载体。我们开发了一种交联反应激活的闪蒸纳米沉淀方法，在闪蒸纳米沉淀过程中发生交联反应，形成交联纳米结构。利用该方法，合成了n -羟基琥珀酰亚胺（NHS）功能化的ros响应型硫酮交联剂（TK-NHS），并利用其交联dab核PAMAM树状大分子G5，形成了G5- tk纳米凝胶。利用动态光散射和透射电镜对所得纳米凝胶进行表征，并评估其细胞相容性、刺激性、细胞摄取和活性氧清除活性。我们证实了这些纳米凝胶清除活性氧的能力，并观察到良好的安全性。G5-TK纳米凝胶可以进一步发展为治疗递送应用的载体，以治疗氧化应激相关的病理状况。

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来源期刊

Biomacromolecules 化学-高分子科学

CiteScore

10.60

自引率

4.80%

发文量

417

审稿时长

1.6 months

期刊介绍： Biomacromolecules is a leading forum for the dissemination of cutting-edge research at the interface of polymer science and biology. Submissions to Biomacromolecules should contain strong elements of innovation in terms of macromolecular design, synthesis and characterization, or in the application of polymer materials to biology and medicine. Topics covered by Biomacromolecules include, but are not exclusively limited to: sustainable polymers, polymers based on natural and renewable resources, degradable polymers, polymer conjugates, polymeric drugs, polymers in biocatalysis, biomacromolecular assembly, biomimetic polymers, polymer-biomineral hybrids, biomimetic-polymer processing, polymer recycling, bioactive polymer surfaces, original polymer design for biomedical applications such as immunotherapy, drug delivery, gene delivery, antimicrobial applications, diagnostic imaging and biosensing, polymers in tissue engineering and regenerative medicine, polymeric scaffolds and hydrogels for cell culture and delivery.