Development of Biginelli-based ZnO-coupled carbomer-gel-coated wound dressing gauze with enhanced antibacterial activity†

Abstract

A multicomponent Biginelli reaction was used to produce biologically active dihydropyrimidones that were then combined with ZnO nanoparticles. Biginelli compounds synthesized with various alkyl chains were characterized using high-resolution mass spectrometry as well as ¹H- and ¹³C-NMR spectroscopy. Efficient antibacterial gels were developed by introducing the prepared Biginelli compounds and ZnO nanoparticles into a carbomer polymer matrix. Antibacterial screening revealed that the ABS-G4 gel exhibited the highest antibacterial potential, with minimum inhibitory concentrations of 16 ± 2 and 12 ± 2 μg mL⁻¹ against Escherichia coli and Staphylococcus aureus, respectively. The ABS-G4 gel was characterized using rheological studies, field-emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, powder X-ray diffraction, and atomic force microscopy. The ABS-G4 gel was showing more antibacterial efficacy toward Gram-positive strains of bacteria than Gram-positive ones. An antibacterial dressing was formed by coating the developed gel onto a gauze dressing.