Lime-induced iron deficiency stimulates a stronger response in tolerant grapevine rootstocks compared to low iron availability

IF 6.8 Q1 PLANT SCIENCES Plant Stress Pub Date : 2025-04-06 DOI:10.1016/j.stress.2025.100841

Sarhan Khalil , Rebeka Strah , Arianna Lodovici , Petr Vojta , Jörg Ziegler , Maruša Pompe Novak , Laura Zanin , Nicola Tomasi , Astrid Forneck , Michaela Griesser

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Abstract

Iron (Fe) is abundant in soil, but its bioavailability can be limited by environmental factors, negatively impacting plant growth and productivity. While root mechanisms for enhancing Fe uptake are well-studied in some model plants, the responses of tolerant and susceptible grapevine rootstocks to low Fe availability remain poorly understood. This study examined the responses of two grapevine rootstocks, Fercal (tolerant) and 3309C (susceptible), to three Fe conditions: direct Fe deficiency (−Fe), induced Fe deficiency through the addition of bicarbonate (+Fe+BIC), and control (+Fe). Our main findings include: 1) more severe leaf symptoms in 3309C than in Fercal independent of the type of stress, 2) overall growth reduction due to direct Fe deficiency (−Fe), while under induced Fe deficiency (+Fe+BIC) Fercal strongly increased root biomass. This observation is supported by the increased expression of root-development related genes VviSAUR66 and VviZAT6, 3) enhanced organic acid contents under induced Fe deficiency (+Fe+BIC) and different organic acids profiles depending on applied stress and genotype, and 4) stronger modulation of gene expression in Fercal root tips, including enhanced expression of Fe mobilization and transport genes (VviOPT3, VviIREG3, VviZIF1). Overall, bicarbonate-induced Fe deficiency (+Fe+BIC) had greater negative effects than direct Fe deficiency (−Fe), with Fercal showing a higher adaptive capability to maintain Fe homeostasis.

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石灰诱导的铁缺乏症与低铁有效性相比，会刺激耐受性葡萄砧木产生更强的反应

土壤中铁（Fe）含量丰富，但其生物利用度受到环境因素的限制，对植物生长和生产力产生负面影响。虽然在一些模式植物中，提高铁吸收的根系机制已经得到了很好的研究，但对耐铁和敏感的葡萄藤砧木对低铁有效性的反应仍然知之甚少。本研究考察了两种葡萄砧木Fercal（耐）和3309C（敏感）对三种铁条件的反应：直接缺铁（−Fe）、通过添加碳酸氢盐（+Fe+BIC）诱导缺铁和对照（+Fe）。我们的主要发现包括：1)与胁迫类型无关，3309C的叶片症状比Fercal更严重；2)由于直接缺铁（−Fe）导致整体生长减少，而在诱导缺铁（+Fe+BIC）下，Fercal的根系生物量显著增加。这一发现的证据包括：根发育相关基因VviSAUR66和VviZAT6的表达增加；3)铁缺乏诱导下（+Fe+BIC）有机酸含量增加；4)铁根尖基因表达调控增强，包括铁动员和运输基因（VviOPT3、VviIREG3、VviZIF1）表达增强。总体而言，碳酸氢盐诱导的铁缺乏（+Fe+BIC）比直接铁缺乏（- Fe）有更大的负面影响，而Fercal表现出更高的维持铁稳态的适应能力。

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来源期刊

Plant Stress PLANT SCIENCES-

CiteScore

5.20

自引率

8.00%

发文量

审稿时长

63 days

期刊介绍： The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.