A novel AIEgen fluorescent probe based on quinoline-malononitrile for monitoring and imaging ClO− and viscosity in biosystem

IF 4.6 2区化学 Q1 SPECTROSCOPY Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-04-03 DOI:10.1016/j.saa.2025.126176

Yiyan Huang , Zhihong Gong , Miaojie Wu , Zhenkuan Tan , Haichang Ding , Yuan Ji , Congbin Fan , Gang Liu , Shouzhi Pu

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Abstract

Hypochlorite (ClO⁻) and viscosity play a pivotal role in biological immune defense systems, it has been demonstrated that alterations in ClO⁻ may link to the modifications in intracellular viscosity. Consequently, it is crucial to develop a tool that can accurately assess these changes of ClO⁻ and viscosity concurrently. In this work, a dual-responsive fluorescent probe QM-WV based on the ICT effect for simultaneous detection of ClO⁻ and viscosity was constructed. QM-WV exhibited a signally turn-on fluorescence signal at 552 nm for ClO⁻ with favorable selectivity. Meanwhile, QM-WV displayed an exceptional ‘off–on’ fluorescence response at 650 nm to viscosity changes in glycerol-ethanol system. More importantly, QM-WV is capable of visually detecting ClO⁻ by using smartphone as a portable signal processing device. Based on its low cytotoxicity and stability under physiological conditions, QM-WV further enabled the detection and imaging of ClO⁻ and viscosity in HeLa cells and zebrafish.

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基于喹啉-丙二腈的新型AIEgen荧光探针用于监测和成像生物系统中的ClO -和粘度

次氯酸盐（ClO-）和粘度在生物免疫防御系统中起着举足轻重的作用，有研究表明，ClO-的变化可能与细胞内粘度的变化有关。因此，开发一种能同时准确评估 ClO- 和粘度变化的工具至关重要。在这项工作中，我们构建了一种基于 ICT 效应的双响应荧光探针 QM-WV，用于同时检测 ClO- 和粘度。QM-WV 在 552 纳米波长处对 ClO- 显示出明显的开启荧光信号，并具有良好的选择性。同时，QM-WV 在 650 纳米波长处对甘油-乙醇体系中的粘度变化显示出特殊的 "关闭-开启 "荧光响应。更重要的是，QM-WV 能够利用智能手机作为便携式信号处理设备，直观地检测 ClO-。基于其低细胞毒性和生理条件下的稳定性，QM-WV 进一步实现了对 HeLa 细胞和斑马鱼中 ClO- 和粘度的检测和成像。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.