Development of a novel near-infrared fluorescent probe based on nile blue for the detection of leucine aminopeptidase as a cancer biomarker

IF 4.2 3区工程技术 Q2 CHEMISTRY, APPLIED Dyes and Pigments Pub Date : 2025-09-01 Epub Date: 2025-04-10 DOI:10.1016/j.dyepig.2025.112827

Marcia Domínguez , David Azorín-Soriano , Jessie García-Fleitas , Jennifer Soler-Beatty , Andrea Bernardos , Alba García-Fernández , Juan F. Blandez , Félix Sancenón , Ramón Martínez-Máñez

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Abstract

We report herein the design and synthesis of a near-infrared fluorescent probe (NB–SO₃-Leu) derived from the Nile Blue (NB) fluorophore scaffold, modified with sulfonic groups to improve solubility and biocompatibility. The mechanism of action of the NB-SO₃-Leu probe was successfully validated in the presence of leucine aminopeptidase (LAP), a widely known cancer biomarker. The fluorescence signal of NB-SO₃-Leu, which was initially low, increased 4-fold after incubation with the LAP enzyme after 15 min. In addition, the NB-SO₃-Leu probe was validated in vitro in 4T1 (mouse mammary carcinoma cells), HeLa (human cervical cancer cells), and SK-Mel-103 (human melanoma cells) with high endogenous LAP levels. This work aims to advance in the development of a new generation of reliable and sensitive cancer detection methods.

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开发基于尼罗河蓝的新型近红外荧光探针，用于检测作为癌症生物标记物的亮氨酸氨肽酶

本文报道了一种近红外荧光探针（NB - so3 - leu）的设计和合成，该探针来源于尼罗河蓝（NB）荧光团支架，用磺酸基修饰以提高溶解度和生物相容性。NB-SO3-Leu探针的作用机制在leucine aminopeptidase （LAP）存在的情况下成功验证。LAP是一种众所周知的癌症生物标志物。NB-SO3-Leu的荧光信号最初较低，经LAP酶孵育15分钟后，荧光信号增加了4倍。此外，NB-SO3-Leu探针在内源性LAP水平较高的4T1（小鼠乳腺癌细胞）、HeLa（人宫颈癌细胞）和SK-Mel-103（人黑色素瘤细胞）中进行了体外验证。这项工作旨在推动新一代可靠和敏感的癌症检测方法的发展。

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来源期刊

Dyes and Pigments 工程技术-材料科学：纺织

CiteScore

8.20

自引率

13.30%

发文量

933

审稿时长

33 days

期刊介绍： Dyes and Pigments covers the scientific and technical aspects of the chemistry and physics of dyes, pigments and their intermediates. Emphasis is placed on the properties of the colouring matters themselves rather than on their applications or the system in which they may be applied. Thus the journal accepts research and review papers on the synthesis of dyes, pigments and intermediates, their physical or chemical properties, e.g. spectroscopic, surface, solution or solid state characteristics, the physical aspects of their preparation, e.g. precipitation, nucleation and growth, crystal formation, liquid crystalline characteristics, their photochemical, ecological or biological properties and the relationship between colour and chemical constitution. However, papers are considered which deal with the more fundamental aspects of colourant application and of the interactions of colourants with substrates or media. The journal will interest a wide variety of workers in a range of disciplines whose work involves dyes, pigments and their intermediates, and provides a platform for investigators with common interests but diverse fields of activity such as cosmetics, reprographics, dye and pigment synthesis, medical research, polymers, etc.