A portable platform integrated with smartphones for rapid lactic acid analysis via ratiometric fluorescent imaging

IF 4.6 2区化学 Q1 SPECTROSCOPY Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-04-11 DOI:10.1016/j.saa.2025.126224

Wen-Ru Wu , Jing Li , Yue Lin , Fang Luo , Cui-Ying Lin , Bin Qiu , Zhen-Yu Lin , Jian Wang , Fang-Zheng Huang

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Abstract

This study presents a smartphone-integrated portable platform for rapid lactic acid (LA) analysis using ratiometric fluorescent imaging. The system employs a dual-emission probe composed of glutathione-modified gold nanoclusters (GSH-AuNCs) and carbon quantum dots (CDs), enabling visual detection via fluorescence color transitions under 365 nm UV excitation. As LA concentration increases, the fluorescence shifts from blue to orange. Smartphone photography and RGB color analysis reveal a nonlinear sigmoidal correlation between the red-to-blue intensity ratio (R/B) and LA concentration, modeled with a coefficient of determination (R² = 0.998). The method exhibits high selectivity and achieves recovery rates of 94.5–104.8 % in sweat samples, validated against LC-MS. This technology offers a novel, on-site solution for food quality assessment and health monitoring applications.

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与智能手机集成的便携式平台，可通过比率荧光成像技术进行快速乳酸分析

本研究提出了一种集成智能手机的便携式平台，用于使用比率荧光成像快速分析乳酸（LA）。该系统采用由谷胱甘肽修饰的金纳米团簇（GSH-AuNCs）和碳量子点（CDs）组成的双发射探针，在365 nm紫外激发下通过荧光色跃迁实现视觉检测。随着LA浓度的增加，荧光从蓝色变为橙色。智能手机摄影和RGB色彩分析显示，红蓝强度比（R/B）与LA浓度呈非线性s型相关，并采用决定系数（R2 = 0.998）建模。该方法具有较高的选择性，对汗液样品的回收率为94.5 ~ 104.8%，经LC-MS验证。该技术为食品质量评估和健康监测应用提供了一种新颖的现场解决方案。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.