Monika Halat, Magdalena Klimek-Chodacka, Agnieszka Domagała, Grzegorz Zając, Tomasz Oleszkiewicz, Josef Kapitán and Rafal Baranski
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引用次数: 0
Abstract
Chiroptical studies of the SpyCas9 protein are extremely rare. Nondestructive methods are needed to characterize its active ribonucleoprotein form. Using Raman optical activity (ROA) and circularly polarized luminescence (CPL), we present a new approach to detect key biomolecules involved in CRISPR-Cas technology while preserving their original nucleolytic activity.
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